Clustering data with hclust algorithm for Pilot Study 13C flux effects when RhoC or RhoA perturbed (13C BCs) (Study ST000282)
ESI GCMS Positive ion mode. (Analysis AN000450)| Metabolite | Structure | F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 | F9 | F10 | F11 | F12 | F13 | F14 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| alpha-Ketoglutarate_12345 | ME703145 | 0.22 | 0.16 | 0.10 | NA | 10.59 | 0.08 | 0.11 | 0.73 | 0.03 | 0.13 | 0.49 | 0.26 | NA | 0.95 |
| Fumarate_1234 | ME703135 | 0.19 | 0.17 | 0.15 | 0.17 | 8.75 | 0.15 | 0.36 | 0.53 | 0.22 | 0.27 | 0.68 | 0.35 | 0.59 | 2.84 |
| Lactate_123_13C_3 | ME703130 | 0.02 | 0.01 | 0.01 | 0.00 | 0.00 | NA | 0.90 | 12.20 | 0.00 | 0.10 | 0.52 | 0.32 | 0.01 | 0.01 |
| Glutamate_12345_13C_2 | ME703158 | 1.42 | 0.88 | 0.31 | 0.16 | 0.16 | 0.08 | 1.06 | 8.74 | 0.35 | 0.95 | 0.12 | 0.21 | 0.08 | 0.07 |
| Lactate_123_13C_2 | ME703129 | 0.55 | 0.32 | 0.43 | 0.33 | 0.44 | 0.36 | 0.94 | 8.36 | 0.34 | 1.06 | 0.61 | 0.51 | 0.36 | 0.12 |
| Pyruvate_123_13C_2 | ME703125 | 0.64 | 0.20 | NA | NA | 0.35 | NA | 1.87 | 5.53 | 0.29 | 0.01 | 0.99 | 0.85 | 0.06 | 0.07 |
| Pyruvate_123_13C_3 | ME703126 | 0.01 | 0.01 | 0.01 | NA | NA | 0.01 | 1.46 | 7.36 | 0.00 | NA | 0.94 | 0.74 | NA | 0.00 |
| Lactate_123 | ME703127 | 1.22 | 0.55 | 0.60 | 0.24 | 1.05 | 0.48 | 0.57 | 1.15 | 0.96 | 5.67 | 0.31 | 0.26 | 0.84 | 0.56 |
| Fumarate_1234_13C_1 | ME703136 | 0.34 | 0.28 | 0.12 | NA | 3.41 | NA | 0.40 | 0.85 | 0.73 | NA | 0.19 | NA | 1.59 | 2.67 |
| Fumarate_1234_13C_4 | ME703139 | 0.19 | 0.17 | NA | NA | 1.23 | NA | NA | 0.05 | 1.53 | 0.49 | NA | NA | 2.76 | 1.59 |
| Fumarate_1234_13C_2 | ME703137 | 0.14 | NA | NA | NA | 0.03 | NA | 0.43 | 1.61 | 0.61 | 0.71 | 1.58 | 1.26 | 1.99 | 1.62 |
| Fumarate_1234_13C_3 | ME703138 | NA | 0.01 | NA | NA | 0.36 | NA | NA | 1.70 | 1.15 | 0.41 | 1.24 | 0.78 | 1.81 | 1.54 |
| alpha-Ketoglutarate_12345_13C_3 | ME703148 | 3.70 | NA | 0.09 | NA | 0.09 | 0.08 | NA | 3.21 | 0.03 | 1.40 | 0.31 | NA | NA | 1.07 |
| Alanine_123_13C_1 | ME703132 | 1.05 | NA | 0.43 | 0.75 | NA | NA | 1.26 | 1.64 | NA | 1.51 | NA | NA | 0.31 | 1.06 |
| Lactate_123_13C_1 | ME703128 | NA | 0.18 | NA | NA | NA | NA | 0.46 | 2.84 | NA | 3.25 | 0.06 | 0.04 | NA | 0.17 |
| Glutamate_12345_13C_4 | ME703160 | 1.55 | 0.68 | 0.70 | 0.28 | 1.37 | 0.62 | 0.14 | 5.19 | 1.41 | 1.36 | 0.05 | 0.05 | 0.13 | 0.06 |
| Pyruvate_123_13C_1 | ME703124 | 1.03 | 0.40 | NA | NA | NA | NA | 0.77 | 4.00 | NA | NA | 0.43 | NA | 0.21 | 0.15 |
| Glutamate_12345 | ME703156 | 1.47 | 1.08 | 0.65 | 0.31 | 1.69 | 0.41 | 2.32 | 3.34 | 0.52 | 0.80 | 0.14 | 0.12 | 0.12 | 0.21 |
| Glutamine_12345_13C_1 | ME703163 | NA | 0.59 | NA | NA | 2.51 | 0.07 | 1.39 | 3.10 | 0.52 | 0.12 | NA | 0.17 | NA | NA |
| Glutamine_12345_13C_3 | ME703166 | NA | 0.97 | 0.00 | 0.02 | NA | 2.27 | 1.14 | NA | 1.63 | 1.26 | NA | NA | 0.04 | 0.90 |
| Glutamine_12345_13C_4 | ME703165 | 4.20 | 1.59 | NA | 0.14 | NA | 0.33 | 0.85 | NA | 0.39 | 0.19 | NA | NA | 0.32 | NA |
| Aspartate_1234_13C_4 | ME703155 | 2.75 | 2.10 | 0.62 | 0.17 | 1.29 | 0.57 | 0.01 | 0.17 | 2.53 | 0.12 | NA | NA | 0.12 | 0.02 |
| Glutamate_12345_13C_5 | ME703161 | 2.49 | 1.47 | 0.96 | 0.34 | 2.18 | 1.08 | 0.01 | 0.13 | 2.35 | 2.32 | 0.00 | 0.00 | 0.20 | 0.11 |
| alpha-Ketoglutarate_12345_13C_5 | ME703150 | 2.00 | 1.03 | 0.83 | NA | 1.24 | 0.96 | NA | 0.24 | 0.43 | 1.78 | NA | NA | NA | 1.06 |
| Glutamine_12345_13C_5 | ME703167 | 2.32 | 1.19 | 0.75 | 0.20 | 1.91 | 0.82 | NA | NA | 1.14 | 0.42 | NA | NA | 0.66 | 0.45 |
| Alanine_123 | ME703131 | 2.34 | 1.43 | 0.51 | 0.18 | 0.85 | 0.52 | 0.94 | 1.57 | 1.15 | 2.75 | 0.27 | 0.26 | 0.75 | 0.40 |
| Glutamate_12345_13C_1 | ME703157 | 2.78 | 2.46 | 0.62 | 0.26 | 0.37 | 0.63 | 0.14 | 2.81 | 0.56 | 2.47 | 0.02 | NA | NA | 0.18 |
| Glutamate_12345_13C_3 | ME703159 | 3.29 | 2.17 | 0.96 | 0.38 | 1.08 | 0.68 | 0.06 | 1.67 | 1.33 | 1.67 | NA | 0.03 | 0.17 | 0.10 |
| Aspartate_1234_13C_3 | ME703154 | 1.30 | 1.46 | 0.27 | 0.09 | 0.61 | 0.29 | 0.94 | 2.71 | 2.57 | 0.08 | NA | NA | 0.03 | 0.13 |
| Aspartate_1234_13C_1 | ME703152 | 2.71 | 2.54 | 0.77 | NA | 0.10 | 0.48 | 0.36 | 2.20 | 0.63 | 0.20 | NA | NA | NA | NA |
| Aspartate_1234_13C_2 | ME703153 | 3.15 | 2.22 | 0.65 | 0.22 | 0.44 | 0.27 | 0.59 | 2.86 | 1.38 | 0.11 | NA | NA | 0.07 | 0.05 |
| Glutamine_12345 | ME703162 | 0.76 | 0.37 | 0.18 | 0.08 | 1.64 | 0.20 | 4.02 | 1.45 | 0.28 | 0.12 | 1.26 | 1.00 | 0.16 | 0.17 |
| Oxaloacetate_1234 | ME703140 | 0.40 | 4.25 | 0.42 | 0.37 | 5.01 | 0.31 | 0.39 | 0.26 | 0.25 | 0.18 | 0.33 | 0.33 | 0.24 | 2.62 |
| Oxaloacetate_1234_13C_1 | ME703141 | NA | 1.96 | 0.72 | 0.08 | 5.04 | 0.35 | 0.40 | 0.18 | 0.19 | NA | NA | 0.09 | NA | NA |
| Oxaloacetate_1234_13C_3 | ME703143 | NA | 0.96 | NA | 0.38 | 3.21 | 1.23 | NA | NA | NA | 0.27 | 0.75 | NA | 0.20 | NA |
| Aspartate_1234 | ME703151 | 1.85 | 1.58 | 0.62 | 0.27 | 3.53 | 0.48 | 2.05 | 1.54 | 0.69 | 0.23 | 0.04 | 0.03 | 0.06 | 0.30 |
| Pyruvate_123 | ME703123 | 1.18 | 0.87 | 1.47 | 0.04 | 3.01 | 1.40 | 0.87 | 0.73 | 1.60 | 0.26 | 0.52 | 0.64 | 0.09 | 0.88 |
Factors:
| F1 | Cell line:MCF10A | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no |
| F2 | Cell line:MCF10A | 13C Label:Glutamine | RhoC overexpression:yes | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no |
| F3 | Cell line:MDA-MB-231 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no |
| F4 | Cell line:MDA-MB-231 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:yes |
| F5 | Cell line:MDA-MB-231 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:yes | RhoC drug:no |
| F6 | Cell line:MDA-MB-231 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:yes | RhoC knockdown:no | RhoC drug:no |
| F7 | Cell line:SUM149 | 13C Label:Glucose | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no |
| F8 | Cell line:SUM149 | 13C Label:Glucose | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:yes |
| F9 | Cell line:SUM149 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no |
| F10 | Cell line:SUM149 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:yes |
| F11 | Cell line:SUM190 | 13C Label:Glucose | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no |
| F12 | Cell line:SUM190 | 13C Label:Glucose | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:yes | RhoC drug:no |
| F13 | Cell line:SUM190 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no |
| F14 | Cell line:SUM190 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:yes | RhoC drug:no |
