Clustering data with hclust algorithm for Trace 13C-glucose, 13C-glutamine, and 13C-serine in genetically engineered pancreatic cell lines through ceramides (part III) (Study ST000644)
Reversed phase POSITIVE ION MODE (Analysis AN000976)| Metabolite | Structure | F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 | F9 | F10 | F11 | F12 | F13 | F14 | F15 | F16 | F17 | F18 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| C16 Ceramide M+2 | ME175151 | 0.22 | 1.57 | NA | 0.06 | 1.19 | 8.22 | 0.07 | 1.26 | 5.86 | 0.09 | 1.09 | NA | 0.03 | 1.06 | NA | 0.04 | 1.05 | NA |
| C16 Ceramide m+28 | ME175177 | 1.54 | 1.08 | NA | 1.24 | NA | 2.24 | 1.20 | NA | 4.05 | 1.17 | NA | NA | 1.00 | 0.22 | 0.19 | 1.21 | 0.28 | 0.18 |
| C16 Ceramide m+29 | ME175178 | 1.74 | 0.88 | NA | 1.02 | NA | NA | 1.13 | NA | 4.58 | 1.27 | NA | NA | 0.86 | 0.14 | 0.16 | 1.05 | 0.22 | NA |
| C18_1 ceramide M+2 | ME175186 | 0.50 | 0.37 | 0.31 | 0.51 | 1.34 | 0.25 | 0.34 | NA | 1.15 | 0.42 | NA | 3.24 | 0.41 | 2.34 | 2.75 | 0.32 | NA | 2.52 |
| C18_1 ceramide m0 | ME175184 | 0.28 | 0.78 | 2.11 | 0.26 | 0.78 | 2.20 | 0.22 | 0.61 | 1.57 | 0.23 | 0.68 | 0.17 | 1.65 | 1.62 | 0.40 | 0.24 | 2.35 | 1.70 |
| C14 Ceramide m+1 | ME175117 | 1.24 | 0.42 | 0.75 | 0.79 | 0.28 | 0.77 | 0.32 | 1.45 | 0.49 | 1.48 | 0.80 | 0.35 | 0.93 | 1.40 | 1.30 | 0.86 | 2.08 | 1.59 |
| C16 Ceramide m0 | ME175149 | 0.50 | 0.06 | 1.63 | 0.71 | 1.01 | 1.10 | 0.69 | 1.01 | 1.10 | 0.66 | 1.07 | 1.63 | 0.70 | 1.10 | 1.62 | 0.70 | 1.08 | 1.62 |
| C14 Ceramide m0 | ME175116 | 0.81 | 0.78 | 1.27 | 1.09 | 0.83 | 1.31 | 0.78 | 0.76 | 1.25 | 0.81 | 0.81 | 1.28 | 1.05 | 0.85 | 1.27 | 0.96 | 0.71 | 1.19 |
| C22 Ceramide m0 | ME175297 | 1.00 | 0.86 | 1.25 | 0.62 | 0.85 | 1.26 | 0.34 | 0.94 | 1.26 | 0.97 | 1.00 | 1.26 | 0.96 | 1.03 | 1.24 | 0.84 | 1.08 | 1.25 |
| Sphingosine m0 | ME175051 | 0.84 | 0.96 | 1.21 | 0.81 | 0.95 | 1.21 | 0.76 | 0.93 | 1.21 | 0.79 | 0.96 | 1.20 | 0.89 | 1.00 | 1.20 | 0.87 | 0.99 | 1.20 |
| C24_1 Ceramide m0 | ME175338 | 0.61 | 0.78 | 1.34 | 0.54 | 0.78 | 1.34 | 0.91 | 0.89 | 1.34 | 0.90 | 0.91 | 1.33 | 0.88 | 0.96 | 1.34 | 0.86 | 0.97 | 1.34 |
| Sphinganine m0 | ME175070 | 0.72 | 1.01 | 1.35 | 0.72 | 1.10 | 1.36 | 0.62 | 0.81 | 1.36 | 0.71 | 1.04 | 1.35 | 0.69 | 0.91 | 1.35 | 0.64 | 0.89 | 1.35 |
| C20 ceramide m+4 | ME175262 | 0.51 | 0.52 | 2.63 | 0.49 | 0.37 | 3.01 | 0.43 | 0.19 | 1.76 | 0.45 | 0.24 | 1.98 | 0.48 | NA | 1.71 | 0.43 | 0.54 | 1.90 |
| C24 Ceramide m0 | ME175381 | 0.31 | 0.61 | 1.79 | 0.38 | 0.64 | 1.79 | 0.68 | 1.18 | 1.78 | 0.62 | 0.79 | 1.78 | 0.31 | 0.66 | 1.80 | 0.30 | 0.77 | 1.80 |
| C18 ceramide m0 | ME175221 | 0.43 | 0.66 | 2.03 | 0.40 | 0.82 | 1.97 | 0.27 | 0.57 | 2.05 | 0.34 | 0.60 | 2.04 | 0.19 | 0.70 | 2.07 | 0.15 | 0.69 | 2.01 |
| C20 ceramide m0 | ME175258 | 0.52 | 0.56 | 1.83 | 0.49 | 0.69 | 1.75 | 0.31 | 0.59 | 2.03 | 0.38 | 0.56 | 2.01 | 0.59 | 0.78 | 1.95 | 0.48 | 0.76 | 1.97 |
| C18_1 ceramide m+3 | ME175187 | 0.07 | 1.44 | 0.35 | 0.08 | 2.48 | 0.48 | 0.10 | 2.65 | 0.11 | 0.12 | 2.83 | 2.57 | 0.11 | 0.91 | 0.33 | 0.11 | NA | 0.23 |
| C24 Ceramide m+1 | ME175382 | 1.50 | 1.22 | 0.85 | 1.16 | 1.23 | 0.77 | 1.10 | 0.37 | 0.80 | 0.78 | 1.83 | 1.73 | 0.21 | 0.77 | NA | 0.34 | 0.91 | NA |
| C18_1 ceramide m+5 | ME175189 | 0.03 | 1.79 | NA | 0.05 | 2.80 | NA | 0.04 | 2.28 | NA | 0.17 | 2.10 | 0.17 | 0.15 | 1.48 | 0.04 | 0.06 | NA | 0.15 |
| C8-Ceramide m0 | ME175089 | 0.22 | 2.41 | NA | 0.24 | 2.15 | NA | 0.13 | 1.73 | NA | 0.18 | 2.41 | NA | 0.29 | NA | NA | 0.25 | NA | NA |
| C16 Ceramide m+3 | ME175152 | 0.31 | 2.80 | 0.05 | 0.24 | 1.67 | 1.92 | 0.35 | 2.04 | 0.16 | 0.51 | 1.08 | NA | 0.17 | 1.60 | NA | 0.20 | 1.50 | 0.04 |
| C20 ceramide m+5 | ME175263 | 0.09 | 2.06 | 1.35 | 0.01 | 1.31 | 0.43 | 0.05 | 2.42 | 0.13 | 0.12 | 2.26 | 0.14 | 0.12 | 2.21 | 0.68 | 0.13 | 2.07 | 0.46 |
| Sphinganine m+1 | ME175071 | 0.48 | 1.47 | 0.42 | 0.66 | 1.83 | 0.10 | 0.04 | 1.44 | 0.07 | 0.45 | 1.84 | 0.23 | 0.38 | 1.73 | 0.32 | 0.34 | 2.56 | 0.43 |
| C18 ceramide m+1 | ME175222 | 0.08 | 2.07 | 0.08 | 0.06 | 2.44 | 0.51 | 0.03 | 2.36 | 0.28 | 0.08 | 2.41 | 0.14 | 0.23 | 2.31 | 0.33 | 0.22 | 2.23 | 0.36 |
| Sphingosine M+2 | ME175053 | 0.14 | 2.51 | 0.09 | 0.17 | 2.62 | 0.13 | 0.13 | 2.80 | 0.05 | 0.19 | 2.59 | 0.11 | 0.16 | 2.03 | 0.08 | 0.12 | 2.20 | 0.01 |
| C18 ceramide m+3 | ME175224 | NA | 2.24 | 0.22 | 0.00 | 1.59 | 0.10 | 0.03 | 2.13 | NA | 0.09 | 2.24 | 0.14 | 0.11 | 1.98 | 0.06 | 0.20 | 2.08 | 0.09 |
| C20 ceramide m+1 | ME175259 | 0.22 | 1.90 | NA | 0.21 | 2.23 | NA | 0.14 | 1.75 | NA | 0.13 | 1.75 | NA | 0.14 | 1.72 | NA | 0.17 | 1.65 | NA |
| C24_1 Ceramide m+1 | ME175339 | NA | 1.96 | 0.01 | 0.79 | 0.72 | 0.21 | 0.76 | 1.27 | 0.70 | NA | 1.82 | 1.25 | NA | 0.26 | NA | NA | 0.38 | NA |
| C18_1 ceramide m+1 | ME175185 | 0.52 | 2.40 | NA | 0.61 | NA | NA | 0.35 | 2.12 | NA | 0.33 | 2.01 | NA | 0.20 | 0.67 | NA | 0.31 | NA | NA |
| C18_1 ceramide m+7 | ME175191 | 0.54 | 1.88 | NA | 0.47 | NA | NA | 0.65 | 2.16 | NA | 0.71 | 1.50 | NA | 0.92 | 1.38 | NA | 0.59 | NA | NA |
| Sphingosine m+1 | ME175052 | 0.70 | 0.90 | NA | 0.47 | 2.14 | 0.69 | 0.14 | 1.17 | 0.52 | 0.83 | 1.03 | 0.68 | 1.26 | 1.57 | 0.15 | 0.49 | 1.85 | 1.18 |
| C14 Ceramide M+2 | ME175118 | 1.00 | 1.50 | 0.32 | 1.07 | 0.98 | 0.25 | NA | 1.77 | 0.38 | 1.30 | 1.76 | 0.28 | NA | 1.38 | 0.20 | NA | 1.65 | 0.10 |
| C18 ceramide m+5 | ME175226 | 0.32 | 1.94 | NA | 0.22 | NA | 0.48 | 0.35 | 2.06 | NA | 0.51 | 2.03 | NA | 0.26 | 1.64 | NA | 0.19 | 1.67 | NA |
| C18 ceramide M+2 | ME175223 | 1.05 | 1.62 | 0.12 | 0.92 | 2.43 | 0.41 | 0.74 | 1.29 | 0.30 | 0.73 | 0.78 | 0.23 | 0.80 | 1.12 | 0.14 | 0.63 | 1.22 | 0.10 |
| C18 ceramide m+7 | ME175228 | 1.20 | 1.34 | NA | 1.00 | 1.40 | NA | 0.99 | 1.29 | NA | 1.45 | 1.14 | NA | 0.57 | 0.83 | NA | 0.52 | 0.78 | NA |
| C24_1 Ceramide m+4 | ME175342 | 1.04 | 1.21 | NA | 1.06 | 1.24 | NA | 1.31 | 0.84 | NA | 1.61 | 0.88 | NA | NA | 0.79 | NA | 0.44 | 0.73 | NA |
| C24 Ceramide m+7 | ME175388 | 0.72 | 1.21 | NA | 1.74 | 1.41 | NA | 1.68 | 0.61 | NA | NA | 1.08 | NA | 0.29 | 1.32 | NA | 0.48 | 1.18 | NA |
| C24_1 Ceramide M+2 | ME175340 | 0.43 | 1.48 | NA | 1.24 | 1.66 | NA | 1.13 | 1.08 | NA | 0.83 | 1.16 | NA | 0.06 | 1.20 | NA | 0.12 | 1.18 | NA |
| C24 Ceramide M+2 | ME175383 | 0.44 | 1.36 | NA | 0.73 | 1.52 | NA | 0.98 | 0.83 | NA | 0.69 | 1.44 | NA | 0.24 | 1.66 | NA | 0.31 | 1.44 | NA |
| C24 Ceramide m+4 | ME175385 | 0.56 | 1.46 | NA | 0.47 | 1.54 | NA | 1.11 | 0.95 | NA | 0.52 | 1.32 | NA | 0.26 | 1.56 | NA | 0.35 | 1.45 | NA |
| C24_1 Ceramide m+3 | ME175341 | NA | 1.03 | NA | 1.02 | 0.93 | NA | NA | 1.03 | NA | 1.63 | 0.88 | NA | 0.80 | 1.11 | NA | 0.29 | 1.19 | NA |
| Sphingosine m+3 | ME175054 | NA | 1.42 | NA | 0.34 | 1.09 | NA | NA | 1.80 | 0.17 | NA | 1.23 | NA | 0.13 | 1.18 | 0.52 | 0.12 | 1.89 | 0.13 |
| Sphingosine m+4 | ME175055 | NA | 1.37 | NA | 0.23 | 1.28 | NA | NA | 1.56 | NA | 0.21 | 1.43 | NA | 0.18 | 1.08 | 0.07 | 0.07 | 0.97 | NA |
| Sphingosine m+6 | ME175057 | NA | 1.45 | NA | NA | 1.25 | NA | 0.50 | 1.53 | NA | 0.56 | 1.33 | NA | 0.54 | 1.02 | NA | 0.45 | 1.07 | NA |
| C16 Ceramide m+1 | ME175150 | 0.40 | 1.63 | NA | 0.10 | 0.90 | NA | 0.05 | 1.12 | NA | NA | 1.70 | NA | NA | 0.72 | NA | NA | 1.17 | NA |
| C16 Ceramide m+4 | ME175153 | NA | 2.06 | NA | NA | 1.06 | NA | NA | 1.05 | 0.00 | NA | 1.01 | NA | 0.05 | 0.91 | NA | 0.06 | 0.88 | NA |
| C20 ceramide m+7 | ME175265 | 0.57 | 1.69 | NA | NA | 2.11 | NA | 0.33 | 1.54 | NA | 0.72 | 1.81 | NA | 0.63 | 1.02 | NA | 0.51 | 0.76 | NA |
| C24 Ceramide m+6 | ME175387 | 0.71 | 1.36 | NA | NA | 1.27 | NA | NA | 1.27 | NA | 0.73 | 0.83 | NA | 0.39 | 1.43 | NA | 0.32 | 1.27 | NA |
| C24 Ceramide m+3 | ME175384 | 0.56 | 1.02 | NA | 0.19 | 1.11 | NA | 0.00 | 0.71 | NA | 1.16 | 1.84 | NA | 0.60 | 1.28 | NA | NA | 1.16 | NA |
| C24 Ceramide m+5 | ME175386 | 0.54 | 1.33 | NA | 0.30 | 1.47 | NA | 0.08 | 0.94 | NA | 1.36 | 1.60 | NA | 0.11 | 1.43 | NA | 0.11 | 1.17 | NA |
| C22 Ceramide m+1 | ME175298 | 1.75 | 1.33 | 0.60 | 0.45 | 0.39 | 0.36 | 0.78 | NA | 0.59 | 0.17 | 1.17 | 0.43 | 2.21 | 1.42 | 0.69 | 2.65 | 0.24 | 0.48 |
| C16 Ceramide m+30 | ME175179 | 2.25 | 0.38 | NA | 1.61 | 0.28 | 0.36 | 1.45 | 0.23 | NA | 1.30 | NA | NA | 1.09 | NA | 0.07 | 1.55 | 0.13 | 0.05 |
| Sphingosine m+12 | ME175063 | 1.13 | NA | NA | 1.12 | NA | 0.01 | 1.45 | NA | NA | 1.37 | NA | 0.13 | 0.96 | NA | 0.19 | 1.15 | NA | 0.12 |
| C16 Ceramide m+16 | ME175165 | 1.34 | NA | NA | 1.31 | NA | NA | 1.43 | NA | 0.05 | 1.02 | NA | 0.05 | 0.85 | NA | NA | 1.00 | NA | 0.02 |
| Sphingosine m+14 | ME175065 | 1.25 | NA | NA | 1.32 | NA | NA | 1.48 | NA | 0.01 | 1.28 | NA | NA | 0.94 | NA | 0.05 | 1.02 | NA | 0.04 |
| Sphingosine m+16 | ME175067 | 1.22 | NA | NA | 1.33 | NA | NA | 1.36 | NA | NA | 1.14 | NA | NA | 0.85 | 0.09 | 0.05 | 1.04 | NA | 0.06 |
| C16 Ceramide m+26 | ME175175 | 1.42 | 0.59 | NA | 1.21 | 0.37 | 0.17 | 1.48 | 0.16 | 0.94 | 1.33 | NA | 0.10 | 1.26 | NA | NA | 1.39 | 0.14 | NA |
| C16 Ceramide m+27 | ME175176 | 1.61 | 0.48 | 0.18 | 1.29 | 0.08 | 0.35 | 1.50 | NA | 0.34 | 1.44 | NA | NA | 1.05 | NA | 0.07 | 1.32 | NA | 0.08 |
| C18 ceramide m+4 | ME175225 | 0.55 | 1.02 | NA | 0.56 | NA | NA | 0.68 | 1.25 | NA | 0.78 | 1.14 | NA | 1.62 | 1.11 | NA | 1.50 | 1.16 | NA |
| C18_1 ceramide m+9 | ME175193 | 0.82 | 1.07 | NA | 0.75 | 1.78 | NA | 1.09 | 0.85 | NA | 1.08 | NA | NA | 0.94 | NA | NA | 0.90 | 1.14 | NA |
| C22 Ceramide M+2 | ME175299 | 1.09 | 0.72 | NA | 0.61 | 1.29 | NA | 0.84 | 1.43 | NA | NA | 0.94 | NA | 1.35 | 0.48 | NA | 1.37 | 0.19 | NA |
| C18_1 ceramide m+4 | ME175188 | 1.15 | 0.69 | NA | 1.16 | 2.06 | NA | 1.08 | 0.57 | NA | 0.71 | 0.84 | 0.13 | 1.71 | 0.07 | NA | 1.39 | NA | NA |
| C20 ceramide M+2 | ME175260 | 1.13 | 1.11 | NA | 1.10 | 1.15 | NA | 0.79 | 0.61 | NA | 0.81 | 1.06 | NA | 1.08 | NA | NA | 0.94 | NA | NA |
| C14 Ceramide m+5 | ME175121 | NA | 0.92 | NA | NA | 0.49 | NA | 2.90 | 0.78 | NA | NA | 1.11 | NA | 1.16 | 0.77 | NA | 1.34 | 0.76 | NA |
| C18 ceramide m+6 | ME175227 | NA | 0.54 | NA | 0.59 | 2.98 | NA | 1.38 | 0.43 | NA | NA | 0.85 | NA | 1.20 | 0.94 | NA | 1.06 | 1.05 | NA |
Factors:
| F1 | experiment:PHGDH B34 + Dox (PKM1) | tracer:13C-glucose | time:48 hr | group ID:PHGDH |
| F2 | experiment:PHGDH B34 + Dox (PKM1) | tracer:13C-glutamine | time:48 hr | group ID:PHGDH |
| F3 | experiment:PHGDH B34 + Dox (PKM1) | tracer:No label | time:0 hr | group ID:PHGDH |
| F4 | experiment:PHGDH B34 - Dox (PKM2) | tracer:13C-glucose | time:48 hr | group ID:PHGDH |
| F5 | experiment:PHGDH B34 - Dox (PKM2) | tracer:13C-glutamine | time:48 hr | group ID:PHGDH |
| F6 | experiment:PHGDH B34 - Dox (PKM2) | tracer:No label | time:0 hr | group ID:PHGDH |
| F7 | experiment:PSAT A13 + Dox (PKM1) | tracer:13C-glucose | time:48 hr | group ID:PSAT |
| F8 | experiment:PSAT A13 + Dox (PKM1) | tracer:13C-glutamine | time:48 hr | group ID:PSAT |
| F9 | experiment:PSAT A13 + Dox (PKM1) | tracer:No label | time:0 hr | group ID:PSAT |
| F10 | experiment:PSAT A13 - Dox (PKM2) | tracer:13C-glucose | time:48 hr | group ID:PSAT |
| F11 | experiment:PSAT A13 - Dox (PKM2) | tracer:13C-glutamine | time:48 hr | group ID:PSAT |
| F12 | experiment:PSAT A13 - Dox (PKM2) | tracer:No label | time:0 hr | group ID:PSAT |
| F13 | experiment:PSPH B8 + Dox (PKM1) | tracer:13C-glucose | time:48 hr | group ID:PSPH |
| F14 | experiment:PSPH B8 + Dox (PKM1) | tracer:13C-glutamine | time:48 hr | group ID:PSPH |
| F15 | experiment:PSPH B8 + Dox (PKM1) | tracer:No label | time:0 hr | group ID:PSPH |
| F16 | experiment:PSPH B8 - Dox (PKM2) | tracer:13C-glucose | time:48 hr | group ID:PSPH |
| F17 | experiment:PSPH B8 - Dox (PKM2) | tracer:13C-glutamine | time:48 hr | group ID:PSPH |
| F18 | experiment:PSPH B8 - Dox (PKM2) | tracer:No label | time:0 hr | group ID:PSPH |
