Clustering data with hclust algorithm for TCA Isotopmer metabolomics of H3K27M Cells grown in regular media, glutamine enriched regular media, and glucose encriched regular media (Part-III) (Study ST001025)
GC POSITIVE ION MODE (Analysis AN001682)Metabolite | Structure | F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 | F9 | F10 | F11 | F12 | F13 | F14 | F15 | F16 | F17 | F18 | F19 | F20 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspartate M+5 | ME250046 | NA | NA | 0.09 | NA | 0.06 | 0.04 | NA | NA | NA | NA | 0.08 | 0.20 | 0.32 | 0.09 | 0.14 | 0.93 | 8.68 | 0.50 | 3.11 | 2.19 |
Citrate M+7 | ME250006 | NA | 3.00 | 0.08 | 1.28 | 8.63 | NA | 0.33 | 0.08 | NA | 0.08 | 1.17 | 0.46 | 0.22 | 0.96 | 0.19 | 0.12 | 0.33 | 0.43 | 0.34 | 0.38 |
Alanine M+2 | ME250039 | 2.21 | 0.26 | 2.22 | 0.32 | 0.10 | 7.24 | 0.69 | 5.75 | 0.65 | 0.16 | 0.67 | 0.17 | 1.32 | 0.19 | 0.14 | 2.12 | 0.12 | 2.07 | 0.15 | 0.04 |
Lactate M+3 | ME250035 | 0.07 | 0.12 | 0.09 | 0.12 | 0.01 | 6.19 | 0.17 | 5.74 | 0.19 | 0.03 | 0.05 | 0.02 | 0.10 | 0.03 | NA | 2.89 | 0.12 | 3.16 | 0.08 | NA |
2-Hydroxyglutarate M+7 | ME250069 | 0.09 | 0.91 | 0.74 | 1.37 | 2.99 | 3.65 | 1.86 | 8.21 | 1.21 | 0.29 | 0.28 | 0.33 | 0.18 | 0.46 | 0.14 | 0.06 | 0.03 | 0.05 | 0.04 | 0.08 |
Ketoglutarate M+6 | ME250013 | NA | NA | NA | NA | 0.06 | 5.64 | NA | 8.32 | NA | 1.28 | 2.26 | 0.07 | 0.42 | 0.12 | 0.15 | 0.14 | 0.80 | 0.14 | 0.90 | 0.53 |
Malate M+4 | ME250030 | NA | 4.89 | 0.01 | 4.83 | NA | NA | 7.06 | NA | 6.03 | NA | 0.03 | 0.43 | 0.05 | 0.84 | 0.04 | 0.05 | 0.57 | 0.03 | 0.82 | 0.08 |
Citrate M+4 | ME250003 | 0.07 | 4.87 | 0.14 | 4.63 | NA | 0.06 | 6.48 | 0.10 | 5.45 | 0.01 | 0.10 | 0.42 | 0.14 | 1.00 | 0.17 | 0.02 | 1.03 | 0.05 | 1.13 | 0.08 |
Succinate M+4 | ME250018 | 0.05 | 4.76 | 0.07 | 4.64 | 0.02 | NA | 6.87 | 0.01 | 5.94 | 0.12 | 0.20 | 0.43 | 0.38 | 0.79 | 0.73 | 0.08 | 0.97 | 0.07 | 1.22 | 0.33 |
Citrate M+5 | ME250004 | NA | NA | 0.22 | 0.80 | NA | 0.16 | 5.20 | 0.17 | 5.90 | NA | 0.42 | 0.37 | 0.63 | 0.49 | 0.09 | 0.17 | 0.86 | 0.38 | 1.91 | 0.19 |
Glutamine M+3 | ME250057 | NA | 0.33 | 0.21 | 0.50 | NA | NA | 4.65 | NA | 5.20 | NA | 0.56 | 0.07 | 0.84 | 0.44 | 2.51 | 0.33 | 0.89 | 0.11 | 1.08 | 0.35 |
Fumarate M+1 | ME250021 | 1.00 | 1.17 | 0.93 | 1.56 | NA | 0.67 | 1.98 | 0.63 | 1.69 | 0.51 | 0.62 | NA | NA | NA | 0.72 | NA | NA | NA | NA | 0.19 |
Ketoglutarate M+1 | ME250008 | 0.84 | 1.74 | 1.31 | 1.77 | 0.57 | 1.05 | 2.44 | 1.22 | 2.25 | 0.37 | 1.45 | 0.92 | 1.95 | 0.79 | 0.64 | 0.13 | 0.76 | 0.19 | 0.67 | 0.59 |
Ketoglutarate M+4 | ME250011 | 0.38 | 1.14 | 1.08 | 1.33 | 0.29 | 0.71 | 2.97 | 1.17 | 2.41 | 0.25 | 1.46 | 0.25 | 2.12 | 0.61 | 0.38 | 0.29 | 1.15 | 0.28 | 1.68 | 0.15 |
Aspartate M+1 | ME250042 | 1.16 | 1.97 | 1.45 | 2.39 | 0.85 | 1.11 | 2.77 | 1.22 | 2.30 | 0.38 | 0.57 | 0.30 | 0.22 | 0.59 | 0.17 | 0.87 | 1.07 | 1.66 | 0.12 | 1.31 |
Fumarate M+2 | ME250022 | 0.82 | 2.14 | 0.96 | 2.49 | 0.08 | 1.14 | 2.76 | 1.15 | 2.64 | 0.27 | 0.60 | 0.25 | 0.36 | 0.72 | 1.02 | 0.78 | 0.92 | 0.61 | 0.71 | 1.42 |
Fumarate M+3 | ME250023 | 0.20 | 2.01 | 0.45 | 2.11 | 0.10 | 0.88 | 2.43 | 0.70 | 2.40 | 0.05 | 0.44 | 0.78 | 0.30 | 0.56 | 1.41 | 0.19 | 1.12 | NA | 1.13 | 1.38 |
Glutamate M+1 | ME250048 | 2.51 | 1.43 | 2.13 | 1.80 | 0.29 | 1.53 | 2.54 | 1.71 | 2.31 | 0.81 | 0.53 | 0.56 | 0.70 | 0.52 | NA | 0.35 | 1.25 | 0.49 | 0.75 | NA |
Malate M+1 | ME250027 | 1.18 | 2.13 | 1.31 | 2.63 | 0.28 | 1.22 | 4.10 | 1.40 | 3.36 | 0.46 | 0.12 | 0.14 | 0.08 | 0.56 | NA | NA | 0.33 | NA | 0.38 | 0.45 |
Citrate M+1 | ME250000 | 1.40 | 1.98 | 1.87 | 2.50 | 0.25 | 1.50 | 2.86 | 1.74 | 2.44 | NA | 0.48 | 0.38 | 0.76 | 0.44 | 0.53 | 0.10 | 0.37 | 0.38 | 0.86 | 0.24 |
Aspartate M+2 | ME250043 | 1.13 | 2.07 | 1.71 | 2.41 | 0.14 | 1.13 | 3.40 | 1.34 | 2.76 | 0.03 | 0.16 | 0.51 | 0.64 | 0.61 | 0.06 | NA | 0.69 | NA | 1.05 | 0.02 |
Malate M+2 | ME250028 | 1.08 | 2.01 | 1.70 | 2.31 | 0.04 | 1.15 | 3.53 | 1.29 | 2.97 | 0.07 | 0.03 | 0.17 | 0.16 | 0.53 | NA | 0.06 | 0.44 | 0.16 | 0.75 | NA |
Malate M+3 | ME250029 | 0.69 | 3.23 | 1.03 | 3.15 | NA | 1.41 | 5.41 | 1.75 | 4.45 | NA | 0.11 | 0.69 | 0.23 | 0.97 | 0.44 | 0.15 | 0.55 | 0.12 | 0.86 | 0.17 |
Succinate M+2 | ME250016 | 1.20 | 2.85 | 2.11 | 3.22 | 0.07 | 2.29 | 4.99 | 2.28 | 4.08 | 0.13 | 0.40 | 0.64 | 0.34 | 0.81 | 0.92 | 0.16 | 0.56 | 0.29 | 0.76 | 0.24 |
2-Hydroxyglutarate M+3 | ME250065 | 0.36 | 2.09 | 0.97 | 2.63 | 0.22 | 0.04 | 4.83 | 0.11 | 4.20 | 0.01 | 1.11 | 0.67 | 0.75 | 0.50 | 0.90 | 0.92 | 0.73 | 0.90 | 0.64 | 0.69 |
2-Hydroxyglutarate M+5 | ME250067 | 0.32 | 1.74 | 0.25 | 2.04 | 0.04 | 0.00 | 3.92 | 0.00 | 3.51 | 0.13 | 0.55 | 0.63 | 1.19 | 0.46 | 0.90 | 1.60 | 0.70 | 1.55 | 0.66 | 0.60 |
Fumarate M+4 | ME250024 | 0.01 | 3.20 | 0.02 | 3.26 | 0.02 | 0.01 | 3.41 | 0.01 | 3.41 | 0.18 | 0.07 | 0.82 | 0.14 | 0.87 | 1.74 | 0.11 | 1.41 | 0.06 | 1.16 | 1.66 |
Succinate M+3 | ME250017 | 0.07 | 2.89 | 0.07 | 2.95 | NA | 0.07 | 3.36 | 0.47 | 3.33 | 0.15 | 0.56 | 0.64 | 1.32 | 0.99 | 1.20 | 0.33 | 0.81 | 0.26 | 1.06 | 0.50 |
Ketoglutarate M+5 | ME250012 | 0.01 | 3.08 | 0.04 | 3.23 | NA | 0.01 | 4.50 | 0.02 | 3.99 | 0.02 | 0.09 | 1.35 | 0.01 | 1.18 | 0.01 | 0.00 | 1.20 | 0.00 | 1.50 | 0.02 |
Glutamate M+3 | ME250050 | NA | 2.97 | 0.10 | 3.39 | 0.02 | 0.06 | 5.73 | 0.08 | 4.79 | 0.03 | 0.23 | 0.68 | 0.06 | 0.70 | 0.08 | 0.36 | 1.42 | 0.34 | 1.29 | 0.19 |
Ketoglutarate M+3 | ME250010 | 0.36 | 2.85 | 0.61 | 3.27 | 0.12 | 0.08 | 5.39 | 0.10 | 4.55 | 0.04 | 0.15 | 0.43 | 0.17 | 0.78 | 0.17 | 0.07 | 2.64 | 0.09 | 1.56 | 1.02 |
Citrate M+3 | ME250002 | 0.33 | 2.12 | 0.69 | 2.17 | 0.14 | 0.55 | 4.15 | 0.72 | 3.61 | 0.05 | 0.26 | 0.14 | NA | 0.86 | NA | 0.16 | 0.90 | 0.51 | 1.27 | 0.17 |
Glutamate M+4 | ME250051 | 0.14 | 2.14 | 0.42 | 2.06 | NA | 0.14 | 4.22 | 0.27 | 3.31 | 0.17 | 0.12 | 0.58 | 0.17 | 0.95 | 0.29 | 0.48 | 3.08 | 0.21 | 1.71 | NA |
Aspartate M+4 | ME250045 | 0.01 | 2.52 | 0.01 | 2.53 | NA | NA | 3.70 | 0.00 | 3.07 | 0.00 | 0.06 | 0.93 | 0.04 | 1.54 | NA | 0.11 | 2.14 | 0.03 | 1.05 | 0.31 |
Glutamate M+5 | ME250052 | 0.00 | 2.46 | 0.00 | 2.40 | 0.00 | NA | 3.48 | NA | 3.01 | NA | 0.00 | 0.48 | 0.00 | 0.82 | 0.01 | NA | 1.53 | NA | 1.62 | 0.04 |
2-Hydroxyglutarate M+2 | ME250064 | 1.47 | 0.23 | 2.67 | 0.49 | NA | 2.33 | 1.41 | 2.23 | 1.30 | 0.38 | 0.76 | 0.11 | 0.45 | 0.48 | 0.16 | 0.61 | NA | 0.35 | NA | 0.26 |
Citrate M+2 | ME250001 | 2.35 | 1.51 | 3.16 | 1.75 | 0.01 | 2.41 | 2.29 | 2.68 | 1.96 | 0.06 | 0.19 | 0.02 | 0.50 | 0.31 | 0.15 | 0.48 | 0.31 | 0.56 | 0.36 | 0.07 |
Glutamate M+2 | ME250049 | 2.66 | 0.92 | 3.41 | 1.15 | 0.04 | 2.52 | 1.57 | 2.89 | 1.30 | NA | 0.41 | 0.21 | 0.76 | 0.62 | 0.02 | 0.72 | 0.71 | 0.63 | 0.92 | 0.12 |
Ketoglutarate M+2 | ME250009 | 2.56 | 0.91 | 3.89 | 1.23 | 0.02 | 2.89 | 1.90 | 3.29 | 1.59 | 0.02 | 0.58 | 0.37 | 1.24 | 0.51 | 0.15 | 0.45 | 0.37 | 0.61 | 0.37 | 0.03 |
Aspartate M+3 | ME250044 | 0.12 | 0.74 | 0.18 | 0.72 | NA | 0.28 | 1.18 | 0.33 | 0.96 | NA | 0.30 | 0.31 | 0.35 | 0.29 | 0.07 | 0.23 | 3.55 | 0.25 | 3.98 | 1.17 |
Glutamine M+7 | ME250061 | NA | 0.54 | NA | NA | NA | NA | 0.77 | NA | 0.41 | NA | 0.14 | 1.31 | 0.14 | NA | 0.14 | NA | 1.35 | 0.14 | 2.28 | NA |
Glutamine M+4 | ME250058 | 0.02 | 1.26 | NA | 1.25 | 0.01 | NA | 1.79 | 0.01 | 1.36 | 0.02 | 0.05 | 1.13 | 0.01 | 1.23 | 0.04 | 0.01 | 2.08 | 0.03 | 1.64 | 0.01 |
Glutamine M+5 | ME250059 | 0.00 | 2.07 | 0.00 | 2.07 | 0.00 | 0.00 | 2.64 | 0.00 | 2.16 | 0.00 | 0.00 | 1.50 | 0.00 | 1.47 | 0.00 | 0.00 | 1.91 | 0.00 | 1.58 | 0.01 |
Glutamine M+2 | ME250056 | 0.78 | 0.26 | 0.49 | 0.41 | 0.30 | 3.36 | 0.86 | 2.55 | 1.00 | 0.25 | 1.32 | 1.35 | 0.73 | 1.54 | 1.22 | 0.47 | 0.48 | 0.70 | 0.53 | 0.56 |
Lactate M+2 | ME250034 | 1.23 | 0.11 | 1.43 | 0.05 | NA | 3.12 | 0.41 | 3.34 | 0.71 | NA | 0.79 | 1.07 | 0.53 | 0.90 | 0.94 | 1.15 | 0.44 | 1.17 | 0.58 | 0.44 |
Alanine M+1 | ME250038 | 0.78 | 0.55 | NA | 0.79 | 0.60 | 3.26 | 2.26 | 2.13 | 1.79 | 1.02 | 0.08 | 1.20 | NA | 0.72 | 0.72 | NA | 0.17 | NA | 0.41 | 0.23 |
Glutamine M+1 | ME250055 | 0.61 | 0.86 | NA | 0.72 | 1.74 | 2.55 | 1.09 | 1.27 | 1.15 | 0.80 | 1.12 | 0.83 | 0.54 | 0.43 | 0.15 | NA | NA | NA | 1.02 | NA |
2-Hydroxyglutarate M+4 | ME250066 | 0.45 | 0.14 | 0.53 | 0.17 | 0.04 | 0.25 | 1.36 | 0.35 | 1.00 | NA | 0.83 | 0.03 | 1.32 | 0.33 | NA | 2.39 | 0.68 | 2.22 | 0.67 | 0.56 |
Citrate M+6 | ME250005 | 0.42 | 1.64 | NA | 1.78 | 0.42 | 0.14 | 1.92 | 0.14 | 1.82 | NA | NA | 1.12 | 1.38 | 1.23 | 1.40 | 0.62 | 0.94 | 0.28 | 0.51 | 0.42 |
Succinate M+1 | ME250015 | 0.43 | 0.94 | 0.44 | 1.34 | 0.47 | 0.63 | 1.31 | 0.99 | 1.24 | 0.46 | 0.17 | 2.37 | 0.33 | 2.36 | 2.34 | 0.28 | 0.75 | 0.21 | 0.89 | 0.67 |
Malate M+5 | ME250031 | 0.71 | NA | 0.42 | NA | 0.53 | 0.44 | NA | 0.79 | NA | 1.57 | 0.15 | 0.82 | 0.23 | 1.48 | 1.97 | 0.76 | 0.97 | 0.49 | 2.37 | 1.80 |
Lactate M+1 | ME250033 | 1.89 | 0.36 | 1.93 | 0.18 | 0.21 | 0.64 | 0.31 | 0.63 | 0.49 | NA | 1.77 | 1.88 | 0.48 | 1.50 | 1.69 | 0.92 | 0.46 | 0.80 | 0.65 | 0.60 |
2-Hydroxyglutarate M+1 | ME250063 | 0.35 | 0.44 | 1.20 | 0.49 | NA | 0.32 | 1.46 | 0.43 | 1.25 | 0.61 | 1.05 | 1.35 | 0.66 | 1.42 | 1.47 | 0.71 | 1.20 | 0.68 | 1.07 | 1.01 |
Alanine M0 | ME250037 | 0.98 | 1.01 | 0.99 | 1.00 | 1.01 | 0.91 | 0.99 | 0.94 | 1.00 | 1.01 | 1.01 | 1.00 | 1.01 | 1.01 | 1.01 | 1.00 | 1.01 | 1.00 | 1.01 | 1.01 |
Lactate M0 | ME250032 | 1.01 | 1.02 | 1.01 | 1.02 | 1.02 | 0.84 | 1.02 | 0.85 | 1.02 | 1.03 | 1.02 | 1.00 | 1.02 | 1.00 | 1.00 | 0.98 | 1.02 | 0.98 | 1.02 | 1.02 |
Glutamine M0 | ME250054 | 1.14 | 0.76 | 1.15 | 0.76 | 1.12 | 1.09 | 0.63 | 1.11 | 0.71 | 1.14 | 1.14 | 0.86 | 1.14 | 0.87 | 1.14 | 1.15 | 0.79 | 1.15 | 0.85 | 1.15 |
2-Hydroxyglutarate M0 | ME250062 | 1.05 | 0.96 | 1.01 | 0.92 | 1.05 | 1.00 | 0.72 | 0.90 | 0.77 | 1.09 | 1.04 | 1.03 | 1.02 | 1.03 | 1.01 | 0.98 | 1.03 | 0.98 | 1.04 | 1.04 |
Citrate M0 | ME249999 | 0.95 | 0.82 | 0.90 | 0.81 | 1.05 | 0.94 | 0.70 | 0.92 | 0.75 | 1.06 | 1.05 | 1.05 | 1.03 | 1.04 | 1.05 | 1.05 | 1.02 | 1.04 | 1.01 | 1.06 |
Aspartate M0 | ME250041 | 1.03 | 0.83 | 1.00 | 0.81 | 1.06 | 1.02 | 0.70 | 1.01 | 0.76 | 1.08 | 1.07 | 1.05 | 1.07 | 1.03 | 1.08 | 1.06 | 0.91 | 1.06 | 0.92 | 1.03 |
Malate M0 | ME250026 | 1.00 | 0.80 | 0.98 | 0.79 | 1.05 | 0.99 | 0.66 | 0.98 | 0.72 | 1.04 | 1.05 | 1.03 | 1.05 | 1.01 | 1.05 | 1.05 | 1.02 | 1.05 | 1.01 | 1.04 |
Glutamate M0 | ME250047 | 0.99 | 0.80 | 0.98 | 0.79 | 1.08 | 1.01 | 0.64 | 1.00 | 0.70 | 1.07 | 1.06 | 1.04 | 1.06 | 1.02 | 1.08 | 1.06 | 0.95 | 1.06 | 0.96 | 1.08 |
Ketoglutarate M0 | ME250007 | 1.02 | 0.81 | 0.98 | 0.80 | 1.05 | 1.01 | 0.67 | 0.99 | 0.72 | 1.07 | 1.04 | 1.03 | 1.03 | 1.01 | 1.07 | 1.08 | 0.97 | 1.07 | 0.97 | 1.06 |
Fumarate M0 | ME250020 | 1.03 | 0.85 | 1.02 | 0.83 | 1.07 | 1.02 | 0.80 | 1.02 | 0.81 | 1.04 | 1.05 | 1.03 | 1.06 | 1.02 | 0.95 | 1.05 | 0.98 | 1.05 | 1.00 | 0.96 |
Succinate M0 | ME250014 | 1.06 | 0.83 | 1.04 | 0.80 | 1.08 | 1.03 | 0.69 | 1.01 | 0.74 | 1.07 | 1.08 | 0.95 | 1.07 | 0.94 | 0.93 | 1.08 | 1.02 | 1.08 | 1.00 | 1.05 |
Factors:
F1 | Sample type:Cells | Grouping:IV 12h GLC |
F2 | Sample type:Cells | Grouping:IV 12h GLN |
F3 | Sample type:Cells | Grouping:IV 24h GLC |
F4 | Sample type:Cells | Grouping:IV 24h GLN |
F5 | Sample type:Cells | Grouping:IV Basal |
F6 | Sample type:Cells | Grouping:XVII 12h GLC |
F7 | Sample type:Cells | Grouping:XVII 12h GLN |
F8 | Sample type:Cells | Grouping:XVII 24h GLC |
F9 | Sample type:Cells | Grouping:XVII 24h GLN |
F10 | Sample type:Cells | Grouping:XVII Basal |
F11 | Sample type:Media | Grouping:IV 12h GLC |
F12 | Sample type:Media | Grouping:IV 12h GLN |
F13 | Sample type:Media | Grouping:IV 24h GLC |
F14 | Sample type:Media | Grouping:IV 24h GLN |
F15 | Sample type:Media | Grouping:IV Basal |
F16 | Sample type:Media | Grouping:XVII 12h GLC |
F17 | Sample type:Media | Grouping:XVII 12h GLN |
F18 | Sample type:Media | Grouping:XVII 24h GLC |
F19 | Sample type:Media | Grouping:XVII 24h GLN |
F20 | Sample type:Media | Grouping:XVII Basal |