Clustering data with hclust algorithm for Targeted Sphingolipid analysis of HeLa silenced for or overexpressing GOLPH3 or LCS (Study ST001673)
Normal phase POSITIVE ION MODE (Analysis AN002731)| Metabolite | Structure | F1 | F2 | F3 | F4 | F5 |
|---|---|---|---|---|---|---|
| C18_1Lact-Cer | ME391047 | 0.98 | 1.18 | 1.21 | 0.78 | 0.85 |
| C20_1Lact-Cer | ME391051 | 0.92 | 1.23 | 1.35 | 0.63 | 0.86 |
| C26_1Lact-Cer | ME391063 | 0.95 | 1.16 | 1.29 | 0.65 | 0.95 |
| C22Lact-Cer | ME391058 | 0.93 | 1.27 | 1.54 | 0.36 | 0.91 |
| C18Lact-Cer | ME391050 | 1.01 | 1.18 | 1.53 | 0.50 | 0.77 |
| C22_1Lact-Cer | ME391055 | 0.90 | 1.28 | 1.60 | 0.45 | 0.77 |
| C24_1Lact-Cer | ME391059 | 0.98 | 1.26 | 1.59 | 0.40 | 0.77 |
| C16Lact-Cer | ME391046 | 1.14 | 1.05 | 1.53 | 0.52 | 0.76 |
| C20Lact-Cer | ME391054 | 0.99 | 1.03 | 1.55 | 0.59 | 0.85 |
| C24Lact-Cer | ME391062 | 1.05 | 1.05 | 1.51 | 0.45 | 0.94 |
| C22_1-Cer | ME391026 | 0.84 | 1.75 | 0.84 | 0.64 | 0.94 |
| C26_1-SM | ME391042 | 0.82 | 1.61 | 0.76 | 0.75 | 1.05 |
| C18_1-Cer | ME391022 | 1.03 | 1.24 | 0.94 | 0.76 | 1.03 |
| C24-SM | ME391041 | 0.94 | 1.32 | 0.82 | 0.80 | 1.12 |
| C22_1-SM | ME391038 | 0.99 | 1.52 | 0.95 | 0.57 | 0.96 |
| C22-SM | ME391039 | 0.98 | 1.35 | 0.92 | 0.76 | 0.99 |
| C24_1-SM | ME391040 | 0.94 | 1.41 | 0.91 | 0.80 | 0.94 |
| C20_1-Cer | ME391024 | 0.97 | 1.52 | 0.91 | 0.72 | 0.89 |
| C20_1-SM | ME391036 | 0.98 | 1.44 | 0.98 | 0.72 | 0.88 |
| C18_1-SM | ME391034 | 1.04 | 1.08 | 1.02 | 0.86 | 0.99 |
| C26Lact-Cer | ME391066 | 0.99 | 1.09 | 1.06 | 0.90 | 0.97 |
| C18_1HexosylCer | ME391049 | 1.00 | 1.06 | 0.97 | 1.01 | 0.96 |
| C26-SM | ME391043 | 0.93 | 1.15 | 0.93 | 0.96 | 1.03 |
| C16-SM | ME391033 | 0.85 | 1.19 | 0.88 | 1.18 | 0.89 |
| C18-SM | ME391035 | 0.95 | 1.16 | 0.95 | 1.08 | 0.86 |
| C26HexosylCer | ME391067 | 0.98 | 1.10 | 0.85 | 1.14 | 0.93 |
| C20-SM | ME391037 | 0.97 | 1.36 | 0.91 | 0.91 | 0.85 |
| C14-SM | ME391032 | 0.85 | 1.34 | 0.88 | 0.99 | 0.94 |
| C18-Cer | ME391023 | 0.89 | 1.24 | 0.89 | 1.06 | 0.92 |
| C22_1HexosylCer | ME391057 | 0.92 | 1.62 | 0.70 | 1.17 | 0.59 |
| C14-Cer | ME391020 | 0.75 | 1.48 | 0.70 | 1.17 | 0.91 |
| C26_1HexosylCer | ME391065 | 0.86 | 1.31 | 0.72 | 1.24 | 0.86 |
| C20-Cer | ME391025 | 0.83 | 1.41 | 0.89 | 1.12 | 0.75 |
| C24-Cer | ME391029 | 0.88 | 1.37 | 0.85 | 1.08 | 0.83 |
| C20_1HexosylCer | ME391053 | 0.93 | 1.38 | 0.79 | 1.19 | 0.71 |
| C26-Cer | ME391031 | 0.94 | 1.32 | 0.88 | 1.14 | 0.72 |
| C26_1-Cer | ME391030 | 0.88 | 1.56 | 0.87 | 0.99 | 0.70 |
| C22-Cer | ME391027 | 0.79 | 1.54 | 0.83 | 1.10 | 0.74 |
| C24_1-Cer | ME391028 | 0.82 | 1.52 | 0.88 | 1.14 | 0.64 |
| C16HexosylCer | ME391048 | 0.64 | 0.87 | 0.47 | 2.52 | 0.51 |
| C18HexosylCer | ME391052 | 0.70 | 0.78 | 0.56 | 2.43 | 0.54 |
| C20HexosylCer | ME391056 | 0.83 | 0.93 | 0.64 | 1.98 | 0.62 |
| C16-Cer | ME391021 | 0.63 | 1.25 | 0.62 | 1.67 | 0.83 |
| C14HexosylCer | ME391045 | 0.76 | 1.16 | 0.62 | 1.82 | 0.64 |
| C24_1HexosylCer | ME391061 | 0.88 | 1.22 | 0.74 | 1.53 | 0.64 |
| C22HexosylCer | ME391060 | 0.83 | 1.15 | 0.65 | 1.68 | 0.68 |
| C24HexosylCer | ME391064 | 0.84 | 1.16 | 0.66 | 1.60 | 0.75 |
Factors:
| F1 | Treatment:GOLPH3 overexpression |
| F2 | Treatment:GOLPH3 silencing |
| F3 | Treatment:LCS overexpression |
| F4 | Treatment:LCS silencing |
| F5 | Treatment:no treatment |
