Data for (Study ST002124)
(Analysis AN003477)Values for each metabolite have been scaled by dividing by the mean across all factors Run Hierarchial cluster analysis on this study | Run Heatmap cluster analysis on this study| Metabolite | F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 | F9 | F10 | F11 | F12 | F13 | F14 | F15 | F16 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| [13C, 15N]Alanine, 2TBDMS | 0.0136 | 1.3636 | 1.1775 | 1.2757 | 0.0174 | 1.7384 | 1.2896 | 1.5036 | 0.0146 | 1.4557 | 1.2375 | 1.3470 | 0.0127 | 1.2662 | 1.1001 | 1.1870 |
| [13C, 15N]Aspartic acid, 3TBDMS | 0.0142 | 1.4171 | 1.3424 | 1.3721 | 0.0169 | 1.6913 | 0.6509 | 1.0308 | 0.0151 | 1.5121 | 1.4171 | 1.4544 | 0.0138 | 1.3785 | 1.3196 | 1.3538 |
| [13C, 15N]Glutamic acid, 3TBDMS | 0.0131 | 1.3118 | 1.0627 | 1.2221 | 0.0276 | 2.7564 | 0.5596 | 1.6273 | 0.0161 | 1.6107 | 1.1092 | 1.4214 | 0.0120 | 1.1989 | 0.9917 | 1.0594 |
| [13C, 15N]Glycine, 2TBDMS | 0.0180 | 1.2520 | 1.0924 | 1.3098 | 0.0207 | 1.9664 | 0.9791 | 1.4046 | 0.0130 | 1.6751 | 1.3028 | 1.4658 | 0.0160 | 1.1329 | 1.1653 | 1.1861 |
| [13C, 15N]Histidine, 3TBDMS | 0.0138 | 1.3848 | 1.2526 | 1.3151 | 0.0179 | 1.7940 | 0.5198 | 1.2347 | 0.0136 | 1.3634 | 1.2312 | 1.2776 | 0.0158 | 1.5760 | 1.4724 | 1.5171 |
| [13C, 15N]Isoleucine, 2TBDMS | 0.0156 | 1.5591 | 1.1819 | 1.3899 | 0.0195 | 1.9461 | 0.6052 | 1.1266 | 0.0162 | 1.6207 | 1.1779 | 1.4115 | 0.0143 | 1.4269 | 1.1181 | 1.3705 |
| [13C, 15N]Leucine, 2TBDMS | 0.0141 | 1.4133 | 1.3006 | 1.3715 | 0.0192 | 1.9155 | 0.6581 | 1.2589 | 0.0140 | 1.4042 | 1.3384 | 1.3620 | 0.0136 | 1.3595 | 1.2413 | 1.3157 |
| [13C, 15N]Lysine, 3TBDMS | 0.0144 | 1.4382 | 1.2073 | 1.2786 | 0.0185 | 1.8485 | 0.9012 | 1.5152 | 0.0140 | 1.3978 | 1.2279 | 1.3058 | 0.0136 | 1.3594 | 1.1941 | 1.2655 |
| [13C, 15N]Methionine, 2TBDMS | 0.0128 | 1.2824 | 1.2190 | 1.2438 | 0.0192 | 1.9240 | 1.0852 | 1.3001 | 0.0152 | 1.5201 | 1.3067 | 1.4248 | 0.0124 | 1.2423 | 1.1942 | 1.1977 |
| [13C, 15N]Phenylalanine, 2TBDMS | 0.0151 | 1.5091 | 1.2519 | 1.2761 | 0.0195 | 1.9458 | 0.7470 | 1.2362 | 0.0177 | 1.7689 | 1.1629 | 1.3267 | 0.0127 | 1.2726 | 1.2044 | 1.2337 |
| [13C, 15N]Proline, 2TBDMS | 0.0133 | 1.3324 | 1.0874 | 1.2051 | 0.0227 | 2.2697 | 0.7274 | 1.5688 | 0.0151 | 1.5052 | 1.1415 | 1.2213 | 0.0138 | 1.3789 | 1.2004 | 1.2972 |
| [13C, 15N]Serine, 3TBDMS | 0.0139 | 1.3891 | 1.2738 | 1.3301 | 0.0146 | 1.4639 | 1.0442 | 1.2348 | 0.0147 | 1.4750 | 1.2722 | 1.3949 | 0.0140 | 1.3960 | 1.3217 | 1.3470 |
| [13C, 15N]Threonine, 3TBDMS | 0.0139 | 1.3897 | 1.1351 | 1.2764 | 0.0201 | 2.0090 | 0.9701 | 1.3179 | 0.0147 | 1.4652 | 1.1933 | 1.3292 | 0.0140 | 1.3972 | 1.1816 | 1.2726 |
| [13C, 15N]Tyrosine, 3TBDMS | 0.0134 | 1.3440 | 1.1316 | 1.2741 | 0.0208 | 2.0794 | 0.9335 | 1.3967 | 0.0137 | 1.3682 | 1.2100 | 1.2984 | 0.0139 | 1.3867 | 1.2271 | 1.2884 |
| [13C, 15N]Valine, 2TBDMS | 0.0160 | 1.5956 | 1.0847 | 1.1623 | 0.0224 | 2.2357 | 0.7549 | 1.3725 | 0.0162 | 1.6230 | 1.1348 | 1.1591 | 0.0157 | 1.5681 | 1.1429 | 1.0960 |
| [13C]Alpha_Ketoglutarate | NA | 0.6382 | 0.7513 | 0.6888 | NA | 0.9762 | 2.7165 | 1.5555 | NA | 0.6606 | 0.8525 | 0.7677 | NA | 0.6665 | 0.9232 | 0.8031 |
| [13C]Citrate | NA | 0.6633 | 0.8826 | 0.7712 | NA | 0.8390 | 2.8952 | 1.6848 | NA | 0.6555 | 0.7804 | 0.7311 | NA | 0.5563 | 0.8260 | 0.7146 |
| [13C]Fumatate | NA | 0.8947 | 0.9948 | 0.9583 | NA | 0.9789 | 1.4557 | 1.2126 | NA | 0.8455 | 0.9599 | 0.8995 | NA | 0.9043 | 0.9694 | 0.9265 |
| [13C]Malate | NA | 0.8587 | 0.9759 | 0.9356 | NA | 1.0901 | 1.5291 | 1.1805 | NA | 0.8767 | 0.9939 | 0.9263 | NA | 0.7730 | 0.9750 | 0.8851 |
| [13C]Succinate | NA | 0.8215 | 0.9757 | 0.8925 | NA | 0.9696 | 1.6308 | 1.2860 | NA | 0.8134 | 1.0507 | 0.9087 | NA | 0.7627 | 1.0000 | 0.8884 |
| Alanine, 2TBDMS | 1.2626 | NA | NA | NA | 0.3280 | NA | NA | NA | 0.9665 | NA | NA | NA | 1.4429 | NA | NA | NA |
| Alpha_Ketoglutarate | 1.1137 | NA | NA | NA | 0.7384 | NA | NA | NA | 1.0495 | NA | NA | NA | 1.0984 | NA | NA | NA |
| Aspartic acid, 3TBDMS | 1.1939 | NA | NA | NA | 0.1832 | NA | NA | NA | 0.9735 | NA | NA | NA | 1.6494 | NA | NA | NA |
| Citrate | 1.1875 | NA | NA | NA | 0.6915 | NA | NA | NA | 1.0014 | NA | NA | NA | 1.1196 | NA | NA | NA |
| Fumatate | 1.0985 | NA | NA | NA | 0.7113 | NA | NA | NA | 1.0740 | NA | NA | NA | 1.1162 | NA | NA | NA |
| Glutamic acid, 3TBDMS | 1.2291 | NA | NA | NA | 0.3224 | NA | NA | NA | 0.9013 | NA | NA | NA | 1.5471 | NA | NA | NA |
| Glutamine, 3TBDMS | 1.2462 | NA | NA | NA | 0.2392 | NA | NA | NA | 0.7723 | NA | NA | NA | 1.7424 | NA | NA | NA |
| Glycine, 2TBDMS | 1.2353 | NA | NA | NA | 0.4972 | NA | NA | NA | 0.9960 | NA | NA | NA | 1.2715 | NA | NA | NA |
| Histidine, 3TBDMS | 1.2408 | NA | NA | NA | 0.2550 | NA | NA | NA | 0.7858 | NA | NA | NA | 1.7184 | NA | NA | NA |
| Hydroxyproline, (E)-, 3TBDMS | 1.1734 | NA | NA | NA | 0.2471 | NA | NA | NA | 0.8776 | NA | NA | NA | 1.7019 | NA | NA | NA |
| Isoleucine, 2TBDMS | 1.2767 | NA | NA | NA | 0.3284 | NA | NA | NA | 0.9370 | NA | NA | NA | 1.4579 | NA | NA | NA |
| L-Cysteine, 3TBDMS | 1.2561 | NA | NA | NA | 0.1824 | NA | NA | NA | 0.7528 | NA | NA | NA | 1.8087 | NA | NA | NA |
| Leucine, 2TBDMS | 1.2818 | NA | NA | NA | 0.3490 | NA | NA | NA | 0.9642 | NA | NA | NA | 1.4051 | NA | NA | NA |
| Lysine, 3TBDMS | 1.2217 | NA | NA | NA | 0.2777 | NA | NA | NA | 0.9868 | NA | NA | NA | 1.5138 | NA | NA | NA |
| Malate | 1.1809 | NA | NA | NA | 0.6192 | NA | NA | NA | 1.0005 | NA | NA | NA | 1.1994 | NA | NA | NA |
| Methionine, 2TBDMS | 1.3454 | NA | NA | NA | 0.2973 | NA | NA | NA | 0.7412 | NA | NA | NA | 1.6161 | NA | NA | NA |
| Phenylalanine, 2TBDMS | 1.3057 | NA | NA | NA | 0.2156 | NA | NA | NA | 0.9113 | NA | NA | NA | 1.5675 | NA | NA | NA |
| Proline, 2TBDMS | 1.1943 | NA | NA | NA | 0.2604 | NA | NA | NA | 0.7566 | NA | NA | NA | 1.7887 | NA | NA | NA |
| Serine, 3TBDMS | 1.3114 | NA | NA | NA | 0.2643 | NA | NA | NA | 0.9970 | NA | NA | NA | 1.4272 | NA | NA | NA |
| Succinate | 1.1254 | NA | NA | NA | 0.7370 | NA | NA | NA | 0.9928 | NA | NA | NA | 1.1447 | NA | NA | NA |
| Threonine, 3TBDMS | 1.1744 | NA | NA | NA | 0.2773 | NA | NA | NA | 0.7323 | NA | NA | NA | 1.8159 | NA | NA | NA |
| Tyrosine, 3TBDMS | 1.1706 | NA | NA | NA | 0.2282 | NA | NA | NA | 1.0174 | NA | NA | NA | 1.5839 | NA | NA | NA |
| Valine, 2TBDMS | 1.3187 | NA | NA | NA | 0.3498 | NA | NA | NA | 0.9705 | NA | NA | NA | 1.3610 | NA | NA | NA |
Factors:
| F1 | Source_Name[gating]:Lineage-negative (Lin–) | Treatment:[13C, 15N] AAs were incorporated for 1 h | Batch:1a | Note:[13C, 15N] AAs were used for AA uptake (Fig1.B); unlabeled AAs were used for total AA (Fig1.A) and TCA substrate levels (Fig1.D). |
| F2 | Source_Name[gating]:Lineage-negative (Lin–) | Treatment:[13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C). |
| F3 | Source_Name[gating]:Lineage-negative (Lin–) | Treatment:[13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C). |
| F4 | Source_Name[gating]:Lineage-negative (Lin–) | Treatment:[13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C). |
| F5 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:[13C, 15N] AAs were incorporated for 1 h | Batch:1a | Note:[13C, 15N] AAs were used for AA uptake (Fig1.B); unlabeled AAs were used for total AA (Fig1.A) and TCA substrate levels (Fig1.D). |
| F6 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D). |
| F7 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D). |
| F8 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D). |
| F9 | Source_Name[gating]:Lin– Scal1+ c-Kit+ (LSK) | Treatment:[13C, 15N] AAs were incorporated for 1 h | Batch:1a | Note:[13C, 15N] AAs were used for AA uptake (Fig1.B); unlabeled AAs were used for total AA (Fig1.A) and TCA substrate levels (Fig1.D). |
| F10 | Source_Name[gating]:Lin– Scal1+ c-Kit+ (LSK) | Treatment:[13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C). |
| F11 | Source_Name[gating]:Lin– Scal1+ c-Kit+ (LSK) | Treatment:[13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C). |
| F12 | Source_Name[gating]:Lin– Scal1+ c-Kit+ (LSK) | Treatment:[13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C). |
| F13 | Source_Name[gating]:Mature hematopoietic cells (Lin+ cells) | Treatment:[13C, 15N] AAs were incorporated for 1 h | Batch:1a | Note:[13C, 15N] AAs were used for AA uptake (Fig1.B); unlabeled AAs were used for total AA (Fig1.A) and TCA substrate levels (Fig1.D). |
| F14 | Source_Name[gating]:Mature hematopoietic cells (Lin+ cells) | Treatment:[13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C). |
| F15 | Source_Name[gating]:Mature hematopoietic cells (Lin+ cells) | Treatment:[13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C). |
| F16 | Source_Name[gating]:Mature hematopoietic cells (Lin+ cells) | Treatment:[13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C). |
