Data for (Study ST002125)
(Analysis AN003478)Values for each metabolite have been scaled by dividing by the mean across all factors Run Hierarchial cluster analysis on this study | Run Heatmap cluster analysis on this study| Metabolite | F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 | F9 | F10 | F11 | F12 | F13 | F14 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| [13C, 15N]Alanine, 2TBDMS | NA | NA | 0.0149 | 1.4922 | 1.3558 | 1.2326 | 0.0148 | 1.4112 | 1.3558 | 1.2264 | 1.4658 | 1.0874 | 1.2678 | 0.0136 |
| [13C, 15N]Aspartic acid, 3TBDMS | NA | NA | 0.0220 | 2.1960 | 0.8985 | 1.2090 | 0.0224 | 1.9551 | 0.8652 | 1.0449 | 1.7390 | 0.6693 | 1.0599 | 0.0154 |
| [13C, 15N]Glutamic acid, 3TBDMS | NA | NA | 0.0287 | 2.8677 | 0.9043 | 1.5277 | 0.0273 | 2.3743 | 0.6733 | 1.2580 | 1.0894 | 0.2212 | 0.6431 | 0.0130 |
| [13C, 15N]Glycine, 2TBDMS | NA | NA | 0.0205 | 1.7714 | 1.1277 | 1.3763 | 0.0201 | 1.7275 | 1.2175 | 1.2991 | 1.4835 | 0.7387 | 1.0597 | 0.0152 |
| [13C, 15N]Histidine, 3TBDMS | NA | NA | 0.0187 | 1.8750 | 1.3708 | 1.5760 | 0.0182 | 1.7711 | 1.2529 | 1.4936 | 1.2719 | 0.3685 | 0.8754 | 0.0153 |
| [13C, 15N]Isoleucine, 2TBDMS | NA | NA | 0.0190 | 1.8987 | 1.1583 | 1.6190 | 0.0182 | 1.7934 | 1.4026 | 1.6059 | 1.2739 | 0.3962 | 0.7375 | 0.0148 |
| [13C, 15N]Leucine, 2TBDMS | NA | NA | 0.0215 | 2.1452 | 0.9279 | 1.4934 | 0.0239 | 2.1112 | 0.8438 | 1.4773 | 1.3645 | 0.4688 | 0.8968 | 0.0145 |
| [13C, 15N]Lysine, 3TBDMS | NA | NA | 0.0190 | 1.8980 | 0.9314 | 1.3276 | 0.0200 | 1.8345 | 0.9258 | 1.1850 | 1.5729 | 0.7669 | 1.2893 | 0.0133 |
| [13C, 15N]Methionine, 2TBDMS | NA | NA | 0.0192 | 1.9197 | 1.2868 | 1.5386 | 0.0168 | 1.6391 | 1.1644 | 1.3218 | 1.3274 | 0.7487 | 0.8969 | 0.0148 |
| [13C, 15N]Phenylalanine, 2TBDMS | NA | NA | 0.0184 | 1.8449 | 0.8651 | 1.3446 | 0.0189 | 1.7496 | 0.9659 | 1.4981 | 1.7802 | 0.6834 | 1.1310 | 0.0160 |
| [13C, 15N]Proline, 2TBDMS | NA | NA | 0.0192 | 1.9227 | 1.1795 | 1.5409 | 0.0220 | 2.1170 | 1.0843 | 1.6965 | 1.1263 | 0.3610 | 0.7785 | 0.0119 |
| [13C, 15N]Serine, 3TBDMS | NA | NA | 0.0176 | 1.7630 | 1.0956 | 1.3876 | 0.0189 | 1.8108 | 1.0655 | 1.4828 | 1.2664 | 0.9033 | 1.0683 | 0.0109 |
| [13C, 15N]Threonine, 3TBDMS | NA | NA | 0.0173 | 1.7344 | 1.0030 | 1.3826 | 0.0178 | 1.6966 | 1.0678 | 1.4860 | 1.6418 | 0.7928 | 1.0770 | 0.0127 |
| [13C, 15N]Tyrosine, 3TBDMS | NA | NA | 0.0214 | 2.1405 | 1.0150 | 1.5872 | 0.0218 | 2.0127 | 0.8240 | 1.5649 | 1.2497 | 0.5610 | 0.8394 | 0.0130 |
| [13C, 15N]Valine, 2TBDMS | NA | NA | 0.0018 | 1.8430 | 1.2641 | 1.5892 | 0.0021 | 1.8720 | 1.1470 | 1.5316 | 1.3500 | 0.4559 | 0.8287 | 0.0012 |
| [13C]Alpha_Ketoglutarate | NA | NA | 4.4562 | 0.0129 | 0.0148 | 0.0146 | 4.4324 | 0.0109 | 0.0131 | 0.0123 | 0.0197 | 0.0549 | 0.0314 | 2.9272 |
| [13C]Citrate | NA | NA | 4.4371 | 0.0096 | 0.0139 | 0.0114 | 4.9335 | 0.0103 | 0.0125 | 0.0110 | 0.0176 | 0.0606 | 0.0353 | 2.4475 |
| [13C]Fumatate | NA | NA | 4.3933 | 0.0086 | 0.0102 | 0.0091 | 4.5956 | 0.0099 | 0.0111 | 0.0111 | 0.0145 | 0.0215 | 0.0179 | 2.8975 |
| [13C]Malate | NA | NA | 4.7555 | 0.0136 | 0.0159 | 0.0153 | 4.9152 | 0.0142 | 0.0158 | 0.0148 | 0.0180 | 0.0253 | 0.0195 | 2.1771 |
| [13C]Succinate | NA | NA | 4.4992 | 0.0073 | 0.0091 | 0.0084 | 4.4946 | 0.0072 | 0.0097 | 0.0085 | 0.0111 | 0.0187 | 0.0147 | 2.9121 |
| Alanine, 2TBDMS | 3.8993 | 0.9554 | 0.0618 | NA | NA | NA | 0.0661 | NA | NA | NA | NA | NA | NA | 0.0173 |
| Alpha_Ketoglutarate | NA | NA | 1.1314 | NA | NA | NA | 1.1254 | NA | NA | NA | NA | NA | NA | 0.7432 |
| Aspartic acid, 3TBDMS | 3.6589 | 0.9618 | 0.1630 | NA | NA | NA | 0.1646 | NA | NA | NA | NA | NA | NA | 0.0516 |
| Citrate | NA | NA | 1.1264 | NA | NA | NA | 1.2524 | NA | NA | NA | NA | NA | NA | 0.6213 |
| Fumatate | NA | NA | 1.1088 | NA | NA | NA | 1.1599 | NA | NA | NA | NA | NA | NA | 0.7313 |
| Glutamic acid, 3TBDMS | 3.2105 | 1.4807 | 0.1268 | NA | NA | NA | 0.1464 | NA | NA | NA | NA | NA | NA | 0.0357 |
| Glutamine, 3TBDMS | 3.5091 | 1.4555 | 0.0164 | NA | NA | NA | 0.0151 | NA | NA | NA | NA | NA | NA | 0.0039 |
| Glycine, 2TBDMS | 3.7996 | 0.8467 | 0.1589 | NA | NA | NA | 0.1290 | NA | NA | NA | NA | NA | NA | 0.0658 |
| Histidine, 3TBDMS | 3.7072 | 1.2833 | 0.0046 | NA | NA | NA | 0.0042 | NA | NA | NA | NA | NA | NA | 0.0008 |
| Hydroxyproline, (E)-, 3TBDMS | 3.2752 | 1.3428 | 0.1712 | NA | NA | NA | 0.1681 | NA | NA | NA | NA | NA | NA | 0.0427 |
| Isoleucine, 2TBDMS | 4.0887 | 0.8357 | 0.0329 | NA | NA | NA | 0.0324 | NA | NA | NA | NA | NA | NA | 0.0102 |
| L-Cysteine, 3TBDMS | 2.9631 | 0.5892 | 0.6260 | NA | NA | NA | 0.6641 | NA | NA | NA | NA | NA | NA | 0.1576 |
| Leucine, 2TBDMS | 3.0268 | 1.6985 | 0.1218 | NA | NA | NA | 0.1158 | NA | NA | NA | NA | NA | NA | 0.0370 |
| Lysine, 3TBDMS | 4.0452 | 0.8915 | 0.0303 | NA | NA | NA | 0.0255 | NA | NA | NA | NA | NA | NA | 0.0074 |
| Malate | NA | NA | 1.2041 | NA | NA | NA | 1.2446 | NA | NA | NA | NA | NA | NA | 0.5513 |
| Methionine, 2TBDMS | 3.9250 | 0.9730 | 0.0436 | NA | NA | NA | 0.0474 | NA | NA | NA | NA | NA | NA | 0.0109 |
| Phenylalanine, 2TBDMS | 4.1917 | 0.7813 | 0.0105 | NA | NA | NA | 0.0128 | NA | NA | NA | NA | NA | NA | 0.0037 |
| Proline, 2TBDMS | 3.6370 | 0.9909 | 0.1662 | NA | NA | NA | 0.1775 | NA | NA | NA | NA | NA | NA | 0.0283 |
| Serine, 3TBDMS | 3.8495 | 0.9925 | 0.0630 | NA | NA | NA | 0.0709 | NA | NA | NA | NA | NA | NA | 0.0242 |
| Succinate | NA | NA | 1.1337 | NA | NA | NA | 1.1325 | NA | NA | NA | NA | NA | NA | 0.7338 |
| Threonine, 3TBDMS | 3.7123 | 1.0986 | 0.0837 | NA | NA | NA | 0.0770 | NA | NA | NA | NA | NA | NA | 0.0284 |
| Tyrosine, 3TBDMS | 3.7077 | 1.2528 | 0.0163 | NA | NA | NA | 0.0187 | NA | NA | NA | NA | NA | NA | 0.0045 |
| Valine, 2TBDMS | 3.8446 | 1.0300 | 0.0561 | NA | NA | NA | 0.0543 | NA | NA | NA | NA | NA | NA | 0.0149 |
Factors:
| F1 | Source_Name[gating]:Bone marrow supernatant | Treatment:5 FU 2 weeks | Batch:1c | Note:Unlabeled AAs were used for total AA (FigS2.D) |
| F2 | Source_Name[gating]:Bone marrow supernatant | Treatment:HSC Basal | Batch:1c | Note:Unlabeled AAs were used for total AA (FigS2.D) |
| F3 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:5 FU 2 weeks, [13C, 15N] AAs were incorporated for 1 h | Batch:1c | Note:[13C, 15N] AAs were used for AA uptake (Fig2.B, Fig5M); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C, Fig5K) and TCA substrate levels (Fig2.K, Fig5J). |
| F4 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| F5 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| F6 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| F7 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 1 h | Batch:1c | Note:[13C, 15N] AAs were used for AA uptake (Fig2.B); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C) and TCA substrate levels (Fig2.K). |
| F8 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| F9 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| F10 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| F11 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D). |
| F12 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D). |
| F13 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D). |
| F14 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:HSC Basal or Ctrl, [13C, 15N] AAs were incorporated for 1 h | Batch:1c | Note:[13C, 15N] AAs were used for AA uptake (Fig2.B, Fig5M); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C, Fig5K) and TCA substrate levels (Fig2.K, Fig5J). |
