Data for (Study ST003604)
(Analysis AN005921)Values for each metabolite have been scaled by dividing by the mean across all factors Run Hierarchial cluster analysis on this study | Run Heatmap cluster analysis on this study| Metabolite | F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 | F9 | F10 |
|---|---|---|---|---|---|---|---|---|---|---|
| 1-4-beta-D-Xylan | 1.8931 | 1.3074 | 0.4810 | 0.5123 | 0.5091 | 0.6592 | 0.8703 | 0.5710 | 1.7984 | 1.0909 |
| 2/3-Phospho-D-glycerate | 0.8434 | 0.9156 | 3.5363 | 0.2301 | 1.2296 | 0.7329 | 0.8763 | 0.2788 | 0.8338 | 0.6075 |
| 2-Hydroxyglutarate/Citramalate | 2.1675 | 1.0849 | 0.9316 | 0.2494 | 0.9597 | 1.2467 | 0.8168 | 0.3175 | 1.2876 | 0.8534 |
| 2-Oxoglutaramate | 0.6561 | 0.9233 | 1.7567 | 0.4647 | 1.0379 | 1.1616 | 1.3182 | 0.9743 | 0.9176 | 0.8663 |
| 2-Oxoglutarate | 3.1581 | 1.4091 | 0.2766 | 0.1755 | 0.5120 | 0.5479 | 1.2155 | 0.2512 | 1.1562 | 0.8887 |
| 2-Oxoglutarate 13C5 | 0.8775 | 0.9500 | 0.8363 | 0.8530 | 0.9231 | 0.9523 | 1.6936 | 0.7288 | 0.9736 | 1.2618 |
| 3-Phosphonooxypyruvate | 1.1055 | 1.0706 | 1.2359 | 0.5135 | 1.1885 | 1.0347 | 1.0041 | 0.8655 | 1.0331 | 0.8781 |
| 3-Sulfino-L-alanine | 0.8740 | 0.7848 | 1.8319 | 0.5574 | 0.9317 | 1.1295 | 0.9078 | 0.9607 | 1.3124 | 0.9250 |
| 4-Pyridoxate | 1.0613 | 0.8023 | 1.9137 | 0.1252 | 1.6456 | 1.5185 | 0.7760 | 0.2278 | 0.9273 | 0.9811 |
| 5-L-Glutamyl-taurine | 0.1646 | 0.5350 | 4.1067 | 1.2837 | 0.1117 | 0.1367 | 1.6481 | 1.5593 | 0.1949 | 0.1131 |
| Adenine 13C5 | NA | 0.0303 | 0.5197 | 0.1478 | 0.8665 | 1.2810 | 0.6320 | 0.7079 | 1.8894 | 1.9851 |
| Adenosine 13C5 | 0.4059 | 0.2609 | 0.3500 | NA | 0.2622 | 0.5430 | 0.1935 | 0.2118 | 0.3552 | 4.3366 |
| ADP | 2.4638 | 1.2485 | 0.2533 | 0.2585 | 1.3636 | 0.6879 | 0.6255 | 0.4993 | 1.3558 | 0.9952 |
| ADP 13C10 | 0.0678 | NA | NA | 0.0501 | 0.6005 | 0.8712 | 0.2074 | 0.7092 | 1.6212 | 2.2628 |
| ADP-D-ribose | 1.2217 | 0.6681 | 0.6894 | 0.3196 | 2.1355 | 2.1671 | 0.3105 | 0.6753 | 1.2985 | 0.6760 |
| a-Linolenic acid (Octadecatrienoic acid) | 0.5745 | 0.5326 | 1.2249 | 0.2063 | 3.1310 | 2.2346 | 0.6801 | 0.3236 | 0.9122 | 0.6476 |
| alpha-D-Glucosamine 1-phosphate | 1.2405 | 1.1677 | 1.2742 | 0.2760 | 1.0262 | 0.9781 | 1.0245 | 0.8804 | 1.2597 | 0.7051 |
| alpha-D-Ribose 1-phosphate | 0.6094 | 0.8241 | 1.1461 | 0.0832 | 2.5296 | 2.7341 | 0.4843 | 0.2596 | 1.0297 | 0.4757 |
| AMP 13C10 | NA | 0.0034 | 0.1079 | 0.0219 | 1.4095 | 1.5633 | 0.5012 | 0.9722 | 1.1466 | 2.0375 |
| Arachidonic acid (Eicosatetraenoic acid) | 0.5887 | 0.9633 | 0.5976 | 0.1985 | 2.7005 | 2.5559 | 0.6976 | 0.2854 | 0.7291 | 0.7200 |
| Ascorbate | 2.4283 | 1.9134 | 0.2868 | 0.0347 | 0.3180 | 1.1508 | 0.5689 | 0.0377 | 0.8914 | 1.4567 |
| beta-L-Arabinose 1-phosphate | 0.6094 | 0.8241 | 1.1461 | 0.0832 | 2.5296 | 2.7341 | 0.4843 | 0.2596 | 1.0297 | 0.4757 |
| Citrate | 1.0624 | 0.4582 | 0.7112 | 0.1004 | 6.8329 | 0.9362 | 0.1000 | 0.0726 | 0.1718 | 0.0961 |
| Citrate 13C1 | NA | 0.5773 | 3.3314 | 0.4506 | 0.5715 | 1.7659 | 0.8025 | 0.7493 | 0.8399 | 0.4137 |
| Citrate 13C2 | 0.8864 | 0.4114 | 2.5805 | 0.3605 | 3.6031 | 0.6275 | 0.3572 | 0.2450 | 0.6232 | 0.4397 |
| Citrate 13C3 | 0.2290 | 0.6391 | 4.3169 | 0.4678 | 1.5995 | 0.5563 | 0.4431 | 0.3168 | 0.3791 | 0.5091 |
| Citrate 13C4 | 0.3444 | 0.2214 | 2.5541 | 0.3338 | 4.0131 | 0.6743 | 0.3402 | 0.5635 | 0.2890 | 0.3244 |
| CMP-N-acetylneuraminate | 1.5732 | 1.1923 | 0.5118 | 0.0623 | 1.3077 | 1.8206 | 0.3517 | 0.1599 | 1.0482 | 1.3112 |
| D-Arabitol | 0.0463 | 0.0382 | 2.2319 | 0.4525 | 0.1839 | 0.1982 | 3.0587 | 2.8174 | 0.5722 | 1.3626 |
| Dehydroascorbate | 0.9407 | 0.5148 | 0.5764 | 0.5343 | 3.4615 | 0.8586 | 0.9484 | 0.8709 | 0.5367 | 0.8098 |
| D-Erythrose 4-phosphate | 0.4355 | 0.3934 | 1.0069 | 0.1259 | 0.8435 | 3.8229 | 0.6110 | 0.4435 | 0.2674 | 0.1754 |
| D-Glucono-1-5-lactone 6-phosphate | 0.3467 | 0.4407 | 1.3676 | 0.3589 | 1.5679 | 5.3850 | 0.2611 | 0.1490 | 0.3020 | 0.3804 |
| D-Glucose | 0.9840 | 0.6377 | 1.4668 | 0.6557 | 1.6907 | 1.4435 | 0.7068 | 0.7118 | 0.8382 | 1.2271 |
| D-Glyceraldehyde 3-phosphate/Glycerone phosphate | 0.4064 | 0.4295 | 1.3277 | 0.4064 | 2.4344 | 3.8128 | 0.5008 | 0.4021 | 0.4057 | 0.4447 |
| Dihomo-gamma-linolenic acid ((8Z-11Z-14Z)-Icosatrienoic acid) | 0.7334 | 0.9323 | 0.6875 | 0.1964 | 2.5470 | 2.3143 | 0.7306 | 0.2748 | 0.8208 | 0.8307 |
| Diphosphate | 0.8134 | 0.9598 | 0.9999 | 0.6477 | 1.4208 | 1.0510 | 1.0216 | 0.7765 | 1.3450 | 1.0045 |
| Docosahexaenoic acid | 0.9710 | 1.1893 | 0.7442 | 0.2034 | 1.9376 | 1.6013 | 0.9787 | 0.3761 | 0.9548 | 0.8542 |
| Docosapentaenoic acid ((7Z-10Z-13Z-16Z-19Z)-Docosa-7-10-13-16-19-pentaenoic acid) | 0.8540 | 1.3455 | 0.4944 | 0.2216 | 1.9032 | 1.7600 | 0.8973 | 0.3880 | 0.9032 | 0.8871 |
| Dodecanoic acid (lauric acid) | 1.0000 | 0.9169 | 1.0796 | 0.9335 | 1.2032 | 1.3756 | 0.8822 | 0.9040 | 0.9083 | 0.8798 |
| D-Ribose | 0.8020 | 0.7647 | 0.9964 | 0.7390 | 1.4404 | 1.1853 | 0.8496 | 0.7530 | 0.8595 | 1.8453 |
| Eicosapentaenoic acid | 0.7919 | 1.2937 | 0.6126 | 0.2935 | 1.8378 | 1.5420 | 1.2744 | 0.4753 | 0.6290 | 0.9560 |
| Ethanolamine phosphate | 1.3016 | 1.0091 | 1.2908 | 0.2637 | 1.3068 | 1.4458 | 0.7715 | 0.6598 | 1.0388 | 0.9029 |
| Fructose bisphosphate 13C6 | 0.8287 | 1.0228 | 1.1456 | 0.9681 | 0.8964 | 1.2906 | 1.1036 | 0.4987 | 1.0514 | 1.1714 |
| Fumarate | 1.4157 | 0.6806 | 0.9725 | 0.4707 | 0.3958 | 0.5957 | 1.1881 | 0.5041 | 0.7503 | 3.3460 |
| Fumarate 13C4 | 0.0259 | 0.1722 | 2.2777 | 0.3027 | 0.1428 | 0.1826 | 1.8961 | 1.1376 | 0.2444 | 1.6542 |
| gamma-L-Glutamyl-L-cysteine | 1.5669 | 0.9608 | 0.7700 | 0.1384 | 1.7923 | 1.0044 | 0.9902 | 0.3440 | 1.3551 | 1.1171 |
| Glutathione 13C10 | 0.5484 | 0.2869 | 4.0457 | 0.2504 | 2.2191 | 1.8453 | 0.2287 | 0.2032 | 0.3056 | 0.2092 |
| Glycerol 3-phosphate | 0.3927 | 0.9354 | 2.1165 | 0.6705 | 0.9246 | 1.0140 | 1.2968 | 1.2611 | 0.6873 | 0.7655 |
| Heptanoic acid | 1.0434 | 0.9716 | 1.0682 | 1.0948 | 1.0270 | 1.0577 | 1.0014 | 0.8878 | 0.8968 | 0.9798 |
| Hexadecanoic acid (palmitic acid) | 1.0760 | 1.0009 | 1.0873 | 0.9101 | 1.0108 | 1.0741 | 0.9659 | 0.9635 | 0.9740 | 0.9366 |
| Hexadecenoic acid (Palmitoleic acid) | 0.7412 | 0.6059 | 1.8763 | 0.4826 | 2.1292 | 1.6365 | 0.8224 | 0.5301 | 0.9158 | 0.6541 |
| Hexanoic acid (caproate) | 0.8985 | 0.9713 | 0.9365 | 0.9269 | 0.9809 | 1.0499 | 1.2057 | 1.1350 | 0.9236 | 1.0004 |
| IMP | 0.3692 | 0.8102 | 2.0175 | 0.1301 | 1.4448 | 2.4676 | 1.3237 | 0.2509 | 0.4930 | 0.8830 |
| Itaconate 13C3 | 1.2221 | 1.0757 | 1.0803 | 0.6389 | 1.1015 | 0.6031 | 1.2834 | 1.2358 | 0.8237 | 0.8598 |
| Itaconate 13C4 | 1.0289 | 0.9459 | 1.0586 | 0.5806 | 1.0608 | 0.7123 | 1.3061 | 1.2540 | 0.9709 | 1.1359 |
| Itaconate 13C5 | 0.8607 | 0.7837 | 0.8191 | 0.8858 | 0.9687 | 1.0779 | 1.1137 | 1.0483 | 1.2136 | 1.4448 |
| Lactate | 0.4630 | 0.9085 | 2.7905 | 0.2404 | 1.0089 | 1.2393 | 1.4286 | 0.3340 | 0.5742 | 1.1042 |
| L-Arabinose | 0.8020 | 0.7647 | 0.9964 | 0.7390 | 1.4404 | 1.1853 | 0.8496 | 0.7530 | 0.8595 | 1.8453 |
| L-Cysteate | 0.8039 | 1.1050 | 0.8929 | 0.9833 | 0.9838 | 0.8826 | 1.0254 | 1.0847 | 1.0751 | 1.0583 |
| Linoleic acid ((9Z,12Z)-Octadecadienoic acid) | 1.0378 | 0.7533 | 1.3882 | 0.2917 | 1.9758 | 1.8451 | 0.8649 | 0.4080 | 0.9578 | 0.7241 |
| L-Selenomethionine | 1.5808 | 1.1713 | 0.8502 | 1.7964 | 1.0781 | 0.4089 | 0.3290 | 0.6620 | 1.0587 | 0.8934 |
| Malate | 0.7762 | 0.9961 | 1.3886 | 0.6205 | 0.4938 | 0.8238 | 1.6197 | 0.5742 | 0.9321 | 1.7788 |
| Malate 13C4 | NA | NA | 2.1762 | 0.4050 | 0.0376 | 0.1359 | 2.2768 | 0.8221 | 0.3384 | 1.8080 |
| Maltose | 0.2181 | 0.2052 | 7.8243 | 0.2495 | 0.4643 | 0.4596 | 0.3885 | 0.4031 | 0.2230 | 0.3593 |
| Mannitol | 2.8813 | 0.0887 | 0.2077 | 0.1355 | 0.3935 | 0.3264 | 0.1222 | 0.4723 | 0.3053 | 5.9785 |
| N-Acetylneuraminate | 1.3684 | 1.2079 | 0.1918 | 0.0250 | 1.1239 | 2.1864 | 0.4227 | 0.3387 | 0.9640 | 1.9633 |
| N-Glycoloyl-neuraminate | 1.4910 | 0.8650 | 0.1816 | 0.1297 | 0.9679 | 1.8125 | 0.3577 | 0.8480 | 1.2774 | 1.7818 |
| N-Methylethanolamine phosphate | 1.5756 | 0.6281 | 2.2499 | 0.1735 | 1.6953 | 1.7308 | 0.5037 | 0.5933 | 0.6807 | 0.5410 |
| Nonanoic acid (pelargonic acid) | 0.9819 | 0.9572 | 1.0666 | 0.9599 | 1.0835 | 1.1174 | 0.9786 | 0.9543 | 0.9565 | 0.9868 |
| Octadecanoic acid (stearic acid) | 1.0819 | 0.9381 | 1.1791 | 0.8764 | 0.9369 | 1.0479 | 0.9114 | 1.0203 | 1.0665 | 1.0035 |
| Octadecenoic acid (Oleic acid) | 1.0158 | 0.6805 | 1.5289 | 0.3436 | 2.0854 | 1.7443 | 0.7554 | 0.4954 | 1.0400 | 0.6301 |
| Octanoic acid (caprylic acid) | 1.0802 | 0.9550 | 1.0577 | 1.0311 | 1.0475 | 0.9968 | 1.0438 | 0.8866 | 0.9050 | 1.0411 |
| Pantothenol | 0.3802 | 0.4077 | 4.0708 | 0.1122 | 0.9675 | 0.5116 | 2.3212 | 0.6954 | 0.6344 | 0.4911 |
| Phosphate | 0.8865 | 0.9701 | 1.0577 | 0.6794 | 1.3222 | 1.1479 | 0.9910 | 0.7262 | 1.2110 | 1.0379 |
| Phosphoglycerate 13C3 | 1.0889 | 0.5107 | 1.1378 | 0.1410 | 2.5829 | 1.6038 | 0.2313 | 0.0977 | 1.2470 | 0.6993 |
| Pyruvate | 1.0235 | 0.7917 | 1.7934 | 0.6827 | 0.6975 | 0.8675 | 2.1202 | 0.9492 | 0.5357 | 0.7469 |
| Ribose phosphate 13C5 | 0.0697 | 0.0603 | 0.3597 | 0.0681 | 1.6711 | 2.1039 | 1.3858 | 0.5063 | 1.2677 | 1.1045 |
| (S)(+)-Allantoin | 1.1151 | 0.7520 | 1.0951 | 0.3183 | 2.1695 | 1.5125 | 0.6866 | 0.5559 | 1.1214 | 0.7512 |
| Sedoheptulose 1-phosphate | 0.2281 | 0.4001 | 2.8863 | 0.3368 | 2.0076 | 3.3501 | 0.5103 | 0.1603 | 0.2679 | 0.4522 |
| Sedoheptulose 1-phosphate 13C7 | 0.9223 | 0.8656 | 0.7602 | 0.2653 | 2.0566 | 1.7832 | 0.7834 | 0.6787 | 1.1043 | 0.9149 |
| sn-glycero-3-Phosphoethanolamine | 1.0225 | 0.4244 | 2.4334 | 0.1779 | 2.8172 | 2.3856 | 0.2693 | 0.1920 | 0.5351 | 0.3182 |
| Sphingosine 1-phosphate | 1.1181 | 0.7000 | 1.9370 | 0.2278 | 1.6392 | 1.4927 | 0.8300 | 0.4679 | 0.9008 | 0.7935 |
| Succinate | 1.3325 | 0.7408 | 0.9126 | 0.1379 | 2.5121 | 1.7706 | 0.6438 | 0.3396 | 1.3361 | 0.5332 |
| Succinate 13C4 | 0.9352 | 0.7975 | 1.2716 | 0.9416 | 1.5640 | 1.1984 | 0.8957 | 0.8616 | 0.6658 | 0.9384 |
| Taurine | 1.4506 | 1.1544 | 1.2553 | 0.5824 | 0.9951 | 1.0827 | 0.9323 | 0.9017 | 0.8034 | 0.6878 |
| Tetradecanoic acid (myristic acid) | 0.9962 | 0.9531 | 1.2050 | 0.8817 | 1.1712 | 1.0521 | 1.0152 | 0.8566 | 0.9579 | 0.9582 |
| Tetradecenoic acid (myristoleic acid) | 1.0449 | 0.8883 | 1.3582 | 0.6537 | 1.6359 | 1.3079 | 0.8954 | 0.6467 | 0.8817 | 0.7990 |
| Thymine | 1.0138 | 1.0034 | 1.0511 | 0.8801 | 0.9881 | 1.0252 | 1.0225 | 0.9352 | 1.0952 | 0.9821 |
| trans-4-Hydroxy-L-proline | 1.1319 | 0.8050 | 1.4867 | 0.3036 | 1.5653 | 1.4029 | 0.9430 | 0.6445 | 1.0850 | 0.8271 |
| UDP-glucose | 1.4473 | 0.7487 | 1.6366 | 0.1333 | 0.9598 | 1.8571 | 0.6145 | 0.2985 | 1.2108 | 1.3446 |
| UDP-N-acetyl-D-glucosamine | 1.5604 | 0.8710 | 1.2045 | 0.1044 | 1.3549 | 1.9584 | 0.6460 | 0.3215 | 1.2068 | 0.9011 |
| UMP | 1.2542 | 0.6391 | 1.2522 | 0.0644 | 1.4543 | 2.3408 | 0.7985 | 0.4575 | 1.3304 | 0.7694 |
| Uracil | 0.6869 | 0.7464 | 1.8298 | 0.5663 | 0.8800 | 2.8103 | 0.7555 | 0.6595 | 0.7163 | 0.6026 |
| Xanthine | 0.8117 | 0.8625 | 1.5478 | 0.1083 | 1.6005 | 1.6577 | 1.5093 | 0.4345 | 0.9524 | 0.6527 |
Factors:
| F1 | time (h):0 | Dead E coli treatment:no | inhibitor:none | | genotype:RagA GTP mutant |
| F2 | time (h):0 | Dead E coli treatment:no | inhibitor:none | | genotype:WT |
| F3 | time (h):18 | Dead E coli treatment:yes | inhibitor:150 nM concanamycin A | | genotype:WT |
| F4 | time (h):18 | Dead E coli treatment:yes | inhibitor:50 nM bafilomycin A1 | | genotype:WT |
| F5 | time (h):18 | Dead E coli treatment:yes | inhibitor:none | | genotype:RagA GTP mutant |
| F6 | time (h):18 | Dead E coli treatment:yes | inhibitor:none | | genotype:WT |
| F7 | time (h):6 | Dead E coli treatment:yes | inhibitor:150 nM concanamycin A | | genotype:WT |
| F8 | time (h):6 | Dead E coli treatment:yes | inhibitor:50 nM bafilomycin A1 | | genotype:WT |
| F9 | time (h):6 | Dead E coli treatment:yes | inhibitor:none | | genotype:RagA GTP mutant |
| F10 | time (h):6 | Dead E coli treatment:yes | inhibitor:none | | genotype:WT |