Data for (Study ST003657)

(Analysis AN006007)

Values for each metabolite have been scaled by dividing by the mean across all factors

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Metabolite	F1	F2	F3	F4	F5	F6	F7	F8	F9	F10	F11	F12	F13	F14	F15	F16	F17	F18	F19	F20	F21	F22	F23	F24
2-Oxoglutarate	NA	NA	NA	0.6769	NA	NA	NA	1.3741	NA	NA	1.3837	NA	NA	NA	NA	0.6649	NA	NA	NA	0.6936	NA	NA	1.0752	NA
3-Methyl-2-oxovalerate	1.4845	1.2331	1.1621	1.7824	0.9985	1.1468	0.9389	1.9385	0.8576	1.0337	1.5316	0.9405	0.8000	0.2996	0.8235	1.2827	0.7255	0.9716	0.3592	0.9990	1.0310	0.3505	0.9931	0.7829
Acetate	0.0489	0.7113	0.0351	1.0271	1.9567	0.0366	0.6510	1.0945	1.9278	0.5135	0.8482	1.8092	0.2600	0.4664	0.1846	1.1772	1.7463	0.2381	0.5890	1.0482	2.0709	0.4282	1.0069	1.7811
Alanine	0.9788	1.4183	1.2175	1.3737	1.2414	0.9714	1.0842	1.3761	1.1546	1.4192	1.0077	1.1841	0.2586	0.7901	0.7225	0.9991	0.9281	0.4868	0.8547	0.8804	0.9202	0.7450	0.8096	0.7675
Arginine	0.9758	1.0431	0.7726	1.1513	1.0687	0.7775	0.6816	1.2634	0.6755	0.8048	0.9975	0.9064	1.1209	1.0788	0.7488	1.0076	0.9967	1.0112	1.2903	1.1090	0.9665	1.6709	0.9420	0.7857
Choline	0.9712	1.4904	0.7616	0.6387	0.8181	0.5721	1.0057	0.6348	1.0328	0.8962	0.8493	0.9361	1.2744	2.0287	0.8867	0.8346	0.9491	1.0639	1.0797	0.6961	0.7997	1.9827	0.6507	0.8691
Citrate	0.9053	0.6832	1.1166	1.6409	0.5500	0.7206	1.9959	2.0423	1.1315	1.3883	1.2839	1.2219	0.2420	0.5100	1.1399	0.5890	0.2767	0.6360	1.8643	0.6852	0.5704	1.4215	0.8352	0.3703
Creatine	NA	1.2148	NA	0.5162	1.2316	NA	1.1945	1.1334	0.7459	1.4257	0.8826	0.9626	NA	1.5354	NA	0.5225	0.6222	NA	1.0789	0.8222	0.5133	1.6278	1.1916	0.7185
Creatine phosphate	NA	0.7101	NA	0.3681	1.4626	NA	1.3460	1.6707	1.3791	0.8006	0.9797	1.1617	NA	1.1316	NA	0.6224	0.8435	NA	0.8939	0.7942	0.5495	1.1420	1.1837	0.8251
Cysteine	NA	1.0311	NA	0.8990	1.1687	NA	0.8025	0.6722	0.4871	1.2051	0.4491	0.9843	NA	1.2741	NA	0.5109	0.6043	NA	1.8475	0.5978	0.8610	2.1280	0.8604	1.4817
Ethanol	0.2497	2.9619	0.2453	1.0333	0.7008	0.2493	1.4790	1.0682	0.6821	1.4541	0.8047	0.6583	0.4130	1.6130	0.3984	1.0614	0.7023	0.3492	1.6057	0.9566	0.7475	1.3806	0.9702	0.7132
Formate	0.3948	0.5693	0.2985	0.6610	0.5816	0.3205	2.0971	1.1758	1.1144	1.2211	0.9171	0.8129	0.4226	0.5288	0.6130	1.0951	0.6665	0.5555	2.5182	1.3857	1.1798	1.3217	1.2543	0.9622
Fumarate	0.5201	1.5729	0.6519	1.1651	1.0777	0.5409	1.5188	1.3066	0.9778	1.5687	0.9321	1.6478	0.5479	1.1068	0.9501	0.5773	0.7317	0.7421	1.3690	0.5825	0.7823	0.9695	0.6408	0.7525
Glucose	0.9076	1.2417	0.6938	0.6860	1.0196	0.7453	1.0516	0.8593	0.9434	1.1469	0.7203	0.8160	1.0418	1.6925	0.8199	0.7035	0.9468	0.9521	1.6948	0.8080	0.8403	1.4658	0.8013	0.9621
Glutamate	0.2729	1.2465	0.3617	1.1025	1.0098	0.5248	1.4180	1.2177	1.0320	0.9160	0.9527	0.9494	0.9710	1.0820	0.8560	1.0555	1.0795	1.3407	0.8511	1.0390	1.0531	1.2225	0.9624	0.8581
Glutamine	0.8687	0.3225	0.2228	0.9687	1.0699	0.5236	0.6052	1.2963	1.3856	0.4615	1.0382	1.3316	1.0835	0.9938	0.5011	1.3130	1.1211	0.5335	1.1596	1.3069	1.2564	1.1820	1.3433	1.1971
Glutathione	1.2911	0.5673	0.8705	0.6862	0.5764	0.8133	1.0225	1.6858	1.2703	1.4289	1.1445	0.7487	0.2821	0.7495	0.6735	0.4594	0.3120	0.2834	1.8780	1.2161	1.3328	2.0838	1.0896	0.8222
Glycine	0.3924	1.0963	0.2388	0.8242	1.1756	0.2913	0.9431	0.9544	1.1963	0.9166	0.7872	1.1010	0.6438	1.3167	0.5229	1.0789	1.2048	0.6050	1.4223	1.0790	1.2583	1.2929	1.0572	1.1819
Histamine	2.2508	0.8443	1.6888	0.0652	1.0657	1.8637	0.8715	0.0902	0.8638	0.8082	0.6802	0.5327	1.7138	2.0617	1.7991	0.0924	0.8249	2.4492	1.3754	0.1254	1.0373	1.5105	0.4345	0.9361
Histidine	0.3558	0.7518	0.3390	0.0881	0.5202	0.3546	0.6672	1.1344	0.5471	0.4820	0.1530	0.5108	0.4050	1.6721	0.2719	0.1116	0.4894	0.3949	3.3368	1.1821	3.0770	3.6690	0.1349	1.4516
Isobutyrate	0.2569	0.5552	0.3446	0.3807	4.7323	0.2465	2.7873	0.2695	1.1016	0.8522	0.4286	1.1355	0.0836	2.5166	0.3405	0.2569	1.0131	0.2110	3.1419	0.1930	0.4010	0.3509	0.2118	0.2559
Isocitrate	NA	0.3503	NA	0.9864	0.5360	NA	2.7379	1.0994	0.8264	1.2770	1.0538	0.6018	NA	0.4773	NA	0.6357	0.2601	NA	2.7287	0.6860	0.4446	1.7845	0.8092	0.8606
Isoleucine	0.6035	1.0920	0.5080	0.9923	1.1852	0.5070	0.6738	1.1620	1.0913	0.7738	0.8883	1.0868	0.7938	1.2382	0.5739	1.0512	1.1607	0.7285	1.4098	1.1720	1.1255	1.1953	1.1677	0.9014
Isopropanol	NA	1.3750	NA	0.8657	1.5558	NA	1.9213	1.4760	0.7047	1.0372	1.1158	1.0389	NA	0.4303	NA	0.7098	0.6564	NA	0.4105	1.2864	0.5856	0.7557	1.2013	0.8813
Lactate	0.4969	1.0021	0.6978	1.3718	1.1925	0.4951	1.3011	1.4592	1.2307	1.1481	1.1374	1.1660	0.2415	0.5289	0.5598	1.3360	1.1204	0.4045	0.7261	1.1654	1.2594	0.5822	1.1119	1.1173
Leucine	0.6695	0.8149	0.5070	1.0115	1.4848	0.4977	0.5155	1.1665	1.1529	0.3635	1.0155	1.1938	0.8733	1.5846	0.6813	0.9700	1.1142	0.7350	1.2698	1.1944	1.0514	1.4260	1.0722	0.8280
Lysine	0.8005	0.8999	0.8285	1.2246	1.1657	0.8329	0.7028	0.9105	1.2317	0.7780	0.9118	1.2167	0.8623	0.8067	0.7915	1.2154	1.3055	0.6059	0.9303	1.1262	1.1741	0.9330	1.2199	1.0342
Methionine	0.8847	0.6292	0.6236	1.2378	1.5580	0.6743	1.1984	1.2327	0.9401	0.8875	1.0965	0.7696	0.9651	0.9957	0.8728	1.1705	0.8623	0.9169	0.9846	1.2599	0.7795	1.2458	1.1875	0.7593
myo-Inositol	0.5903	1.3690	0.4970	0.7955	0.9566	0.5666	1.2965	0.6404	1.4307	1.4242	0.4406	1.0150	0.2169	1.7971	0.2564	0.6032	1.2007	0.3742	1.7733	0.7825	0.9456	1.6522	0.7413	1.0619
Phenylalanine	0.8649	0.6498	0.7061	1.2148	1.0084	0.7344	0.7856	1.3047	1.0416	0.6049	1.0589	0.8389	0.8013	1.4410	0.7827	1.1894	1.0318	0.8886	1.3993	1.2608	1.1225	1.2516	1.2209	0.4857
Proline	NA	0.8539	NA	0.5239	1.1001	NA	1.7009	0.7336	1.1419	1.4657	0.6178	1.2690	NA	0.7679	NA	0.5306	0.6381	NA	1.4853	0.7453	0.9351	1.6144	0.7428	1.0143
Pyroglutamate	0.8069	1.2215	0.7888	1.0114	1.2176	0.7060	0.8101	1.0172	1.1206	1.1730	0.7536	0.9905	0.7758	1.2047	0.7335	0.9347	1.2069	0.7696	0.8764	0.9005	1.1171	1.1972	0.8767	1.1415
Pyruvate	0.1795	1.8860	0.1698	1.0726	0.9914	0.1556	1.5292	1.2200	1.1085	1.0457	0.9308	1.0180	0.1265	1.8063	0.3671	0.8671	0.8171	0.2330	1.8674	0.9149	1.1237	0.9914	0.8627	0.8607
Serine	1.0395	1.0282	0.5107	1.0415	0.9587	0.7211	1.3177	1.2031	0.4750	0.9160	0.9019	0.6991	1.1440	1.5747	0.7392	0.6275	0.9670	0.8764	1.6134	0.7768	1.0285	1.4208	0.9275	1.0370
Succinate	0.2924	2.2528	0.2096	0.3460	0.6633	0.1926	2.7715	0.9841	0.6510	1.7827	0.7661	1.0610	0.3104	1.3512	0.3000	0.5752	0.7798	0.3086	2.4135	0.6488	0.9945	0.9180	0.5645	0.9467
Threonine	0.6439	0.9082	0.5341	1.0121	1.2348	0.5188	0.7953	1.1513	1.2650	1.0025	0.9019	1.1111	0.7734	1.2539	0.6630	0.9067	1.1740	0.7233	0.9989	1.0088	1.2129	1.1144	0.9918	1.1597
Tryptophan	1.0723	0.5729	0.7666	1.3646	0.9760	0.8487	0.3942	1.3744	1.2450	0.6750	1.2156	1.1252	1.1898	0.8788	0.5635	1.3715	0.9577	0.9582	0.8186	1.2970	1.0376	0.6913	1.3573	1.0820
Tyrosine	1.0330	0.6097	0.8565	1.4081	1.2475	0.8536	0.7373	1.2997	0.9740	0.7092	0.7442	1.0535	1.1365	0.8258	0.9717	1.1998	0.9765	1.0572	0.9698	1.1882	1.1132	1.0610	1.1981	0.8880
Uracil	0.6302	2.4781	0.7287	0.3077	1.7970	0.6761	2.2713	0.4589	1.0979	1.8479	0.3092	1.3244	NA	1.4795	NA	0.3323	0.8665	NA	2.1343	0.4569	1.3923	1.4303	0.2147	0.7615
Valine	0.5709	1.0503	0.4857	0.8996	1.2084	0.4634	0.9552	1.0430	1.2038	0.8988	0.8366	1.1375	0.7316	1.2819	0.5780	0.9451	1.1871	0.6398	1.2671	1.0122	1.1588	1.1347	1.0054	1.1870

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Factors:

F1	Sample source:U251 | Condition:anti-GLS1 siRNA | Method of exposure:Transfection
F2	Sample source:U251 | Condition:Control | Method of exposure:Direct exposure
F3	Sample source:U251 | Condition:Control | Method of exposure:Transfection
F4	Sample source:U251 | Condition:Control | Method of exposure:Transwell b.End3
F5	Sample source:U251 | Condition:Control | Method of exposure:Transwell b.End3 + iNHA
F6	Sample source:U251 | Condition:Lipofectamine | Method of exposure:Transfection
F7	Sample source:U251 | Condition:PUREG4-LA12-anti-GLS1 siRNA | Method of exposure:Direct exposure
F8	Sample source:U251 | Condition:PUREG4-LA12-anti-GLS1 siRNA | Method of exposure:Transwell b.End3
F9	Sample source:U251 | Condition:PUREG4-LA12-anti-GLS1 siRNA | Method of exposure:Transwell b.End3 + iNHA
F10	Sample source:U251 | Condition:PUREG4-LA12 | Method of exposure:Direct exposure
F11	Sample source:U251 | Condition:PUREG4-LA12 | Method of exposure:Transwell b.End3
F12	Sample source:U251 | Condition:PUREG4-LA12 | Method of exposure:Transwell b.End3 + iNHA
F13	Sample source:U-87MG | Condition:anti-GLS1 siRNA | Method of exposure:Transfection
F14	Sample source:U-87MG | Condition:Control | Method of exposure:Direct exposure
F15	Sample source:U-87MG | Condition:Control | Method of exposure:Transfection
F16	Sample source:U-87MG | Condition:Control | Method of exposure:Transwell b.End3
F17	Sample source:U-87MG | Condition:Control | Method of exposure:Transwell b.End3 + iNHA
F18	Sample source:U-87MG | Condition:Lipofectamine | Method of exposure:Transfection
F19	Sample source:U-87MG | Condition:PUREG4-LA12-anti-GLS1 siRNA | Method of exposure:Direct exposure
F20	Sample source:U-87MG | Condition:PUREG4-LA12-anti-GLS1 siRNA | Method of exposure:Transwell b.End3
F21	Sample source:U-87MG | Condition:PUREG4-LA12-anti-GLS1 siRNA | Method of exposure:Transwell b.End3 + iNHA
F22	Sample source:U-87MG | Condition:PUREG4-LA12 | Method of exposure:Direct exposure
F23	Sample source:U-87MG | Condition:PUREG4-LA12 | Method of exposure:Transwell b.End3
F24	Sample source:U-87MG | Condition:PUREG4-LA12 | Method of exposure:Transwell b.End3 + iNHA