Sample groups(2): Chemical Treatment:DEHP; Chemical Treatment:DMSO

Perform data analysis on user-uploaded data

STEP 1: Load your data file (tab-delimited text file or csv file)     Load example file   Metabolite names have been classified

File format required:
Column 1: sample names
Column 2: group identifier (letters, numbers or text)
Columns 3 to n: Variables
View input file

STEP 2: Choose a method below
Normalization and scaling
Sample normalization:
Normalization method:
Analyte scaling:
Scaling method:
Relative log abundance plot:
Mode:
Bargraphs and Boxplots
Bargraph:
 ANALYTE:
Bargraph ratio:
ANALYTE1:
ANALYTE2:
Boxplot:
 ANALYTE:
Univariate Analysis
Volcano Plot:
Select one or more experimental factors for each of Groups 1 and 2.
The members of each group should be DIFFERENT.
Group1Experimental factorGroup2
Chemical Treatment:DEHP(10)
Chemical Treatment:DMSO(10)

P-value cutoff:     Fold-change cutoff:
Classified Volcano Plot Run Volcano plot and class enrichment analysis on dataset containing RefMet-classified metabolites
Classified Dot Plot Perform multi-condition dot plot analysis on dataset containing RefMet-classified metabolites
ANOVA analysis: Select 2 or more experimental factors for ANOVA analysis.
GroupExperimental factor
Chemical Treatment:DEHP(10)
Chemical Treatment:DMSO(10)

P-value cutoff:
Debiased sparse partial correlation analysis: Select groups for DSPC analysis.
GroupExperimental factor
Chemical Treatment:DEHP(10)
Chemical Treatment:DMSO(10)

Correlation coefficient cutoff:   DSPC p-value cutoff:
Show negative correlations in red:    Hide unconnected nodes:
Clustering and Correlation
T-test/Wilcoxon test:
ANALYTE:
  Method:
Hierarchical Cluster Analysis:
Cluster analysis:
Pearson Correlation:
ANALYTE1:
ANALYTE2:
Clustered correlation analysis:
Correlation coefficient cutoff (0 to 1):
Multivariate Analysis
Principal Component Analysis:
Select 2 or more experimental factors for PCA analysis.
GroupExperimental factor
Chemical Treatment:DEHP(10)
Chemical Treatment:DMSO(10)
Classification and Feature Analysis
OPLS-DA/VIP analysis:
Select one or more experimental factors for each of Groups 1 and 2.
The members of each group should be DIFFERENT.
Group1Experimental factorGroup2
Chemical Treatment:DEHP(10)
Chemical Treatment:DMSO(10)
Random Forest/VIP analysis:
Select 2 or more experimental factors for Random Forest analysis.
GroupExperimental factor
Chemical Treatment:DEHP(10)
Chemical Treatment:DMSO(10)
Number of analytes to display results:
Data matrix (input file)
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