Sample groups(26): Mode:C18 Negative | Ratio:0;100; Mode:C18 Negative | Ratio:100;0; Mode:C18 Negative | Ratio:1024;1; Mode:C18 Negative | Ratio:16;1; Mode:C18 Negative | Ratio:1;1; Mode:C18 Negative | Ratio:1;1024; Mode:C18 Negative | Ratio:1;16; Mode:C18 Negative | Ratio:1;256; Mode:C18 Negative | Ratio:1;4; Mode:C18 Negative | Ratio:1;64; Mode:C18 Negative | Ratio:256;1; Mode:C18 Negative | Ratio:4;1; Mode:C18 Negative | Ratio:64;1; Mode:C18 Positive | Ratio:0;100; Mode:C18 Positive | Ratio:100;0; Mode:C18 Positive | Ratio:1024;1; Mode:C18 Positive | Ratio:16;1; Mode:C18 Positive | Ratio:1;1; Mode:C18 Positive | Ratio:1;1024; Mode:C18 Positive | Ratio:1;16; Mode:C18 Positive | Ratio:1;256; Mode:C18 Positive | Ratio:1;4; Mode:C18 Positive | Ratio:1;64; Mode:C18 Positive | Ratio:256;1; Mode:C18 Positive | Ratio:4;1; Mode:C18 Positive | Ratio:64;1

Perform data analysis on user-uploaded data

STEP 1: Load your data file (tab-delimited text file or csv file)     Load example file    Classify metabolite names via RefMet

File format required:
Column 1: sample names
Column 2: group identifier (letters, numbers or text)
Columns 3 to n: Variables
View input file

STEP 2: Choose a method below
Normalization and scaling
Sample normalization:
Normalization method:
Analyte scaling:
Scaling method:
Relative log abundance plot:
Mode:
Bargraphs and Boxplots
Bargraph:
 ANALYTE:
Bargraph ratio:
ANALYTE1:
ANALYTE2:
Boxplot:
 ANALYTE:
Univariate Analysis
Volcano Plot:
Select one or more experimental factors for each of Groups 1 and 2.
The members of each group should be DIFFERENT.
Group1Experimental factorGroup2
Mode:C18 Negative Ratio:0;100(3)
Mode:C18 Negative Ratio:100;0(3)
Mode:C18 Negative Ratio:1024;1(3)
Mode:C18 Negative Ratio:16;1(3)
Mode:C18 Negative Ratio:1;1(23)
Mode:C18 Negative Ratio:1;1024(3)
Mode:C18 Negative Ratio:1;16(3)
Mode:C18 Negative Ratio:1;256(3)
Mode:C18 Negative Ratio:1;4(3)
Mode:C18 Negative Ratio:1;64(3)
Mode:C18 Negative Ratio:256;1(3)
Mode:C18 Negative Ratio:4;1(3)
Mode:C18 Negative Ratio:64;1(3)
Mode:C18 Positive Ratio:0;100(3)
Mode:C18 Positive Ratio:100;0(3)
Mode:C18 Positive Ratio:1024;1(3)
Mode:C18 Positive Ratio:16;1(3)
Mode:C18 Positive Ratio:1;1(23)
Mode:C18 Positive Ratio:1;1024(3)
Mode:C18 Positive Ratio:1;16(3)
Mode:C18 Positive Ratio:1;256(3)
Mode:C18 Positive Ratio:1;4(3)
Mode:C18 Positive Ratio:1;64(3)
Mode:C18 Positive Ratio:256;1(3)
Mode:C18 Positive Ratio:4;1(3)
Mode:C18 Positive Ratio:64;1(3)

P-value cutoff:     Fold-change cutoff:
ANOVA analysis: Select 2 or more experimental factors for ANOVA analysis.
GroupExperimental factor
Mode:C18 Negative Ratio:0;100(3)
Mode:C18 Negative Ratio:100;0(3)
Mode:C18 Negative Ratio:1024;1(3)
Mode:C18 Negative Ratio:16;1(3)
Mode:C18 Negative Ratio:1;1(23)
Mode:C18 Negative Ratio:1;1024(3)
Mode:C18 Negative Ratio:1;16(3)
Mode:C18 Negative Ratio:1;256(3)
Mode:C18 Negative Ratio:1;4(3)
Mode:C18 Negative Ratio:1;64(3)
Mode:C18 Negative Ratio:256;1(3)
Mode:C18 Negative Ratio:4;1(3)
Mode:C18 Negative Ratio:64;1(3)
Mode:C18 Positive Ratio:0;100(3)
Mode:C18 Positive Ratio:100;0(3)
Mode:C18 Positive Ratio:1024;1(3)
Mode:C18 Positive Ratio:16;1(3)
Mode:C18 Positive Ratio:1;1(23)
Mode:C18 Positive Ratio:1;1024(3)
Mode:C18 Positive Ratio:1;16(3)
Mode:C18 Positive Ratio:1;256(3)
Mode:C18 Positive Ratio:1;4(3)
Mode:C18 Positive Ratio:1;64(3)
Mode:C18 Positive Ratio:256;1(3)
Mode:C18 Positive Ratio:4;1(3)
Mode:C18 Positive Ratio:64;1(3)

P-value cutoff:
Debiased sparse partial correlation analysis: Select groups for DSPC analysis.
GroupExperimental factor
Mode:C18 Negative Ratio:0;100(3)
Mode:C18 Negative Ratio:100;0(3)
Mode:C18 Negative Ratio:1024;1(3)
Mode:C18 Negative Ratio:16;1(3)
Mode:C18 Negative Ratio:1;1(23)
Mode:C18 Negative Ratio:1;1024(3)
Mode:C18 Negative Ratio:1;16(3)
Mode:C18 Negative Ratio:1;256(3)
Mode:C18 Negative Ratio:1;4(3)
Mode:C18 Negative Ratio:1;64(3)
Mode:C18 Negative Ratio:256;1(3)
Mode:C18 Negative Ratio:4;1(3)
Mode:C18 Negative Ratio:64;1(3)
Mode:C18 Positive Ratio:0;100(3)
Mode:C18 Positive Ratio:100;0(3)
Mode:C18 Positive Ratio:1024;1(3)
Mode:C18 Positive Ratio:16;1(3)
Mode:C18 Positive Ratio:1;1(23)
Mode:C18 Positive Ratio:1;1024(3)
Mode:C18 Positive Ratio:1;16(3)
Mode:C18 Positive Ratio:1;256(3)
Mode:C18 Positive Ratio:1;4(3)
Mode:C18 Positive Ratio:1;64(3)
Mode:C18 Positive Ratio:256;1(3)
Mode:C18 Positive Ratio:4;1(3)
Mode:C18 Positive Ratio:64;1(3)

Correlation coefficient cutoff:   DSPC p-value cutoff:
Show negative correlations in red:    Hide unconnected nodes:
Clustering and Correlation
T-test/Wilcoxon test:
ANALYTE:
  Method:
Hierarchical Cluster Analysis:
Cluster analysis:
Pearson Correlation:
ANALYTE1:
ANALYTE2:
Clustered correlation analysis:
Correlation coefficient cutoff (0 to 1):
Multivariate Analysis
Principal Component Analysis:
Select 2 or more experimental factors for PCA analysis.
GroupExperimental factor
Mode:C18 Negative Ratio:0;100(3)
Mode:C18 Negative Ratio:100;0(3)
Mode:C18 Negative Ratio:1024;1(3)
Mode:C18 Negative Ratio:16;1(3)
Mode:C18 Negative Ratio:1;1(23)
Mode:C18 Negative Ratio:1;1024(3)
Mode:C18 Negative Ratio:1;16(3)
Mode:C18 Negative Ratio:1;256(3)
Mode:C18 Negative Ratio:1;4(3)
Mode:C18 Negative Ratio:1;64(3)
Mode:C18 Negative Ratio:256;1(3)
Mode:C18 Negative Ratio:4;1(3)
Mode:C18 Negative Ratio:64;1(3)
Mode:C18 Positive Ratio:0;100(3)
Mode:C18 Positive Ratio:100;0(3)
Mode:C18 Positive Ratio:1024;1(3)
Mode:C18 Positive Ratio:16;1(3)
Mode:C18 Positive Ratio:1;1(23)
Mode:C18 Positive Ratio:1;1024(3)
Mode:C18 Positive Ratio:1;16(3)
Mode:C18 Positive Ratio:1;256(3)
Mode:C18 Positive Ratio:1;4(3)
Mode:C18 Positive Ratio:1;64(3)
Mode:C18 Positive Ratio:256;1(3)
Mode:C18 Positive Ratio:4;1(3)
Mode:C18 Positive Ratio:64;1(3)
Linear Discriminant Analysis:
Select 3 or more experimental factors for LDA analysis.
GroupExperimental factor
Mode:C18 Negative Ratio:0;100(3)
Mode:C18 Negative Ratio:100;0(3)
Mode:C18 Negative Ratio:1024;1(3)
Mode:C18 Negative Ratio:16;1(3)
Mode:C18 Negative Ratio:1;1(23)
Mode:C18 Negative Ratio:1;1024(3)
Mode:C18 Negative Ratio:1;16(3)
Mode:C18 Negative Ratio:1;256(3)
Mode:C18 Negative Ratio:1;4(3)
Mode:C18 Negative Ratio:1;64(3)
Mode:C18 Negative Ratio:256;1(3)
Mode:C18 Negative Ratio:4;1(3)
Mode:C18 Negative Ratio:64;1(3)
Mode:C18 Positive Ratio:0;100(3)
Mode:C18 Positive Ratio:100;0(3)
Mode:C18 Positive Ratio:1024;1(3)
Mode:C18 Positive Ratio:16;1(3)
Mode:C18 Positive Ratio:1;1(23)
Mode:C18 Positive Ratio:1;1024(3)
Mode:C18 Positive Ratio:1;16(3)
Mode:C18 Positive Ratio:1;256(3)
Mode:C18 Positive Ratio:1;4(3)
Mode:C18 Positive Ratio:1;64(3)
Mode:C18 Positive Ratio:256;1(3)
Mode:C18 Positive Ratio:4;1(3)
Mode:C18 Positive Ratio:64;1(3)
Classification and Feature Analysis
OPLS-DA/VIP analysis:
Select one or more experimental factors for each of Groups 1 and 2.
The members of each group should be DIFFERENT.
Group1Experimental factorGroup2
Mode:C18 Negative Ratio:0;100(3)
Mode:C18 Negative Ratio:100;0(3)
Mode:C18 Negative Ratio:1024;1(3)
Mode:C18 Negative Ratio:16;1(3)
Mode:C18 Negative Ratio:1;1(23)
Mode:C18 Negative Ratio:1;1024(3)
Mode:C18 Negative Ratio:1;16(3)
Mode:C18 Negative Ratio:1;256(3)
Mode:C18 Negative Ratio:1;4(3)
Mode:C18 Negative Ratio:1;64(3)
Mode:C18 Negative Ratio:256;1(3)
Mode:C18 Negative Ratio:4;1(3)
Mode:C18 Negative Ratio:64;1(3)
Mode:C18 Positive Ratio:0;100(3)
Mode:C18 Positive Ratio:100;0(3)
Mode:C18 Positive Ratio:1024;1(3)
Mode:C18 Positive Ratio:16;1(3)
Mode:C18 Positive Ratio:1;1(23)
Mode:C18 Positive Ratio:1;1024(3)
Mode:C18 Positive Ratio:1;16(3)
Mode:C18 Positive Ratio:1;256(3)
Mode:C18 Positive Ratio:1;4(3)
Mode:C18 Positive Ratio:1;64(3)
Mode:C18 Positive Ratio:256;1(3)
Mode:C18 Positive Ratio:4;1(3)
Mode:C18 Positive Ratio:64;1(3)
Random Forest/VIP analysis:
Select 2 or more experimental factors for Random Forest analysis.
GroupExperimental factor
Mode:C18 Negative Ratio:0;100(3)
Mode:C18 Negative Ratio:100;0(3)
Mode:C18 Negative Ratio:1024;1(3)
Mode:C18 Negative Ratio:16;1(3)
Mode:C18 Negative Ratio:1;1(23)
Mode:C18 Negative Ratio:1;1024(3)
Mode:C18 Negative Ratio:1;16(3)
Mode:C18 Negative Ratio:1;256(3)
Mode:C18 Negative Ratio:1;4(3)
Mode:C18 Negative Ratio:1;64(3)
Mode:C18 Negative Ratio:256;1(3)
Mode:C18 Negative Ratio:4;1(3)
Mode:C18 Negative Ratio:64;1(3)
Mode:C18 Positive Ratio:0;100(3)
Mode:C18 Positive Ratio:100;0(3)
Mode:C18 Positive Ratio:1024;1(3)
Mode:C18 Positive Ratio:16;1(3)
Mode:C18 Positive Ratio:1;1(23)
Mode:C18 Positive Ratio:1;1024(3)
Mode:C18 Positive Ratio:1;16(3)
Mode:C18 Positive Ratio:1;256(3)
Mode:C18 Positive Ratio:1;4(3)
Mode:C18 Positive Ratio:1;64(3)
Mode:C18 Positive Ratio:256;1(3)
Mode:C18 Positive Ratio:4;1(3)
Mode:C18 Positive Ratio:64;1(3)
Number of analytes to display results:
Data matrix (input file)
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