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Perform data analysis on Reversed phase POSITIVE ION MODE dataset in study ST002757
Choose experimental groups (required for Volcano plot, OPLSDA, Random Forest analyses)
Select 2 different experimental groups:
Select only groups with 3 or more replicates listed in parentheses
Group1
Sample Type:Blank | Batch:1 (8)
Sample Type:Blank | Batch:2 (8)
Sample Type:Blank | Batch:3 (8)
Sample Type:Blank | Batch:4 (8)
Sample Type:Blank | Batch:5 (8)
Sample Type:Blank | Batch:6 (7)
Sample Type:CHEAR | Batch:1 (4)
Sample Type:CHEAR | Batch:2 (4)
Sample Type:CHEAR | Batch:3 (4)
Sample Type:CHEAR | Batch:4 (4)
Sample Type:CHEAR | Batch:5 (4)
Sample Type:CHEAR | Batch:6 (4)
Sample Type:HHEAR U1 | Batch:1 (4)
Sample Type:HHEAR U1 | Batch:2 (4)
Sample Type:HHEAR U1 | Batch:3 (4)
Sample Type:HHEAR U1 | Batch:4 (4)
Sample Type:HHEAR U1 | Batch:5 (4)
Sample Type:HHEAR U1 | Batch:6 (4)
Sample Type:HHEAR U2 | Batch:1 (4)
Sample Type:HHEAR U2 | Batch:2 (4)
Sample Type:HHEAR U2 | Batch:3 (4)
Sample Type:HHEAR U2 | Batch:4 (4)
Sample Type:HHEAR U2 | Batch:5 (4)
Sample Type:HHEAR U2 | Batch:6 (3)
Sample Type:NIST | Batch:1 (1)
Sample Type:NIST | Batch:2 (3)
Sample Type:NIST | Batch:3 (3)
Sample Type:NIST | Batch:4 (3)
Sample Type:NIST | Batch:5 (3)
Sample Type:NIST | Batch:6 (3)
Sample Type:Study pool | Batch:1 (8)
Sample Type:Study pool | Batch:2 (8)
Sample Type:Study pool | Batch:3 (8)
Sample Type:Study pool | Batch:4 (8)
Sample Type:Study pool | Batch:5 (8)
Sample Type:Study pool | Batch:6 (7)
Sample Type:Study sample | Batch:1 (80)
Sample Type:Study sample | Batch:2 (80)
Sample Type:Study sample | Batch:3 (80)
Sample Type:Study sample | Batch:4 (80)
Sample Type:Study sample | Batch:5 (80)
Sample Type:Study sample | Batch:6 (63)
Group2
Sample Type:Blank | Batch:1 (8)
Sample Type:Blank | Batch:2 (8)
Sample Type:Blank | Batch:3 (8)
Sample Type:Blank | Batch:4 (8)
Sample Type:Blank | Batch:5 (8)
Sample Type:Blank | Batch:6 (7)
Sample Type:CHEAR | Batch:1 (4)
Sample Type:CHEAR | Batch:2 (4)
Sample Type:CHEAR | Batch:3 (4)
Sample Type:CHEAR | Batch:4 (4)
Sample Type:CHEAR | Batch:5 (4)
Sample Type:CHEAR | Batch:6 (4)
Sample Type:HHEAR U1 | Batch:1 (4)
Sample Type:HHEAR U1 | Batch:2 (4)
Sample Type:HHEAR U1 | Batch:3 (4)
Sample Type:HHEAR U1 | Batch:4 (4)
Sample Type:HHEAR U1 | Batch:5 (4)
Sample Type:HHEAR U1 | Batch:6 (4)
Sample Type:HHEAR U2 | Batch:1 (4)
Sample Type:HHEAR U2 | Batch:2 (4)
Sample Type:HHEAR U2 | Batch:3 (4)
Sample Type:HHEAR U2 | Batch:4 (4)
Sample Type:HHEAR U2 | Batch:5 (4)
Sample Type:HHEAR U2 | Batch:6 (3)
Sample Type:NIST | Batch:1 (1)
Sample Type:NIST | Batch:2 (3)
Sample Type:NIST | Batch:3 (3)
Sample Type:NIST | Batch:4 (3)
Sample Type:NIST | Batch:5 (3)
Sample Type:NIST | Batch:6 (3)
Sample Type:Study pool | Batch:1 (8)
Sample Type:Study pool | Batch:2 (8)
Sample Type:Study pool | Batch:3 (8)
Sample Type:Study pool | Batch:4 (8)
Sample Type:Study pool | Batch:5 (8)
Sample Type:Study pool | Batch:6 (7)
Sample Type:Study sample | Batch:1 (80)
Sample Type:Study sample | Batch:2 (80)
Sample Type:Study sample | Batch:3 (80)
Sample Type:Study sample | Batch:4 (80)
Sample Type:Study sample | Batch:5 (80)
Sample Type:Study sample | Batch:6 (63)
Filtering Parameters
Select threshold % for selection below:
100% (option 4 only)
95%
90%
85%
80%
75%
70%
65%
60%
55%
50%
45%
40%
35%
30%
20%
10%
0%
Option 1:
Filter by Sum
(Analytes whose sum for all samples falls below the threshold % will be excluded)
Option 2:
Filter by Mean
(Analytes whose mean for all samples falls below the threshold % will be excluded)
Option 3:
Filter by Median
(Analytes whose median for all samples falls below the threshold % will be excluded)
Option 4:
Filter by Sample threshold
At least one of the 2 selected groups
Both groups
must exceed the above threshold per analyte
In order for an analyte to be retained, the % of samples in one/both groups which are non-zero must exceed the threshold.
Option 5:
Remove all analytes containing null values
Sample Normalization Parameters
Normalize samples by:
None
Sample means
Sample sums
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