Perform Volcano plot analysis on selected experimental conditions followed by MS search on significant features

Group1Experimental factorGroup2
288000 (1)
2070000 (1)
5120000 (1)
5390000 (1)
6110000 (1)
6660000 (1)
6670000 (1)
6690000 (1)
6830000 (1)
7010000 (1)
7040000 (1)
7170000 (1)
7200000 (1)
7320000 (1)
7620000 (1)
7630000 (1)
7770000 (1)
8010000 (1)
8040000 (1)
8150000 (1)
8330000 (1)
8340000 (1)
8600000 (1)
8710000 (1)
8810000 (1)
9110000 (1)
9140000 (1)
9220000 (1)
9520000 (1)
9770000 (1)
10700000 (1)
1.1e+07 (1)
11200000 (1)

Filtering Parameters
Select threshold % for selection below:
Option 1: No filtering
Option 2: Filter by Sum (Analytes whose sum for all samples falls below the threshold % will be excluded)
Option 3: Filter by Mean (Analytes whose mean for all samples falls below the threshold % will be excluded)
Option 4: Filter by Median (Analytes whose median for all samples falls below the threshold % will be excluded)
Option 5: Filter by Sample threshold       must exceed the above threshold per analyte
In order for an analyte to be retained, the % of samples in one/both groups which are non-zero must exceed the threshold.
Option 6: Remove all analytes containing null values
Sample Normalization Parameters
Normalize samples by:
Volcano Plot Parameters
P-value cutoff: Fold-change cutoff:
MS Search Parameters
Search Refmet database
MS Ion polarity:NEGATIVE MS search m/z tolerance:    Feature order:
Ion adducts(choose at least one)
Negative mode:
 [M-H]- [M+Cl]- [M+HCOO]- [M+OAc]- [M-CH3]- [M-2H]2- [M-3H]3- [M-H-Ser]-
  logo