Perform Volcano plot analysis on selected experimental conditions followed by MS search on significant features

Group1Experimental factorGroup2
Cell:- | Additive 1:- | Additive 2:- | Environment:- | NO status:- | Time:- (5)
Cell:mutant | Additive 1:Glucose | Additive 2:none | Environment:undefined | NO status:NO | Time:1.5 h (3)
Cell:mutant | Additive 1:Glucose | Additive 2:none | Environment:undefined | NO status:NO | Time:1 h (3)
Cell:mutant | Additive 1:Glucose | Additive 2:none | Environment:undefined | NO status:NO | Time:5 h (3)
Cell:mutant | Additive 1:Glucose | Additive 2:none | Environment:undefined | NO status:NO | Time:6 h (3)
Cell:normal | Additive 1:AA | Additive 2:none | Environment:Aerobic | NO status:not specified | Time:not specified (3)
Cell:normal | Additive 1:AA | Additive 2:none | Environment:undefined | NO status:NO | Time:0.25 h (3)
Cell:normal | Additive 1:AA | Additive 2:none | Environment:undefined | NO status:NO | Time:0.5 h (3)
Cell:normal | Additive 1:AA | Additive 2:none | Environment:undefined | NO status:NO | Time:1.5 h (3)
Cell:normal | Additive 1:AA | Additive 2:none | Environment:undefined | NO status:NO | Time:2.5 h (3)
Cell:normal | Additive 1:AA | Additive 2:none | Environment:undefined | NO status:NO | Time:2 h (3)
Cell:normal | Additive 1:AA | Additive 2:none | Environment:undefined | NO status:NO | Time:3 h (6)
Cell:normal | Additive 1:AA | Additive 2:none | Environment:undefined | NO status:NO | Time:4 h (6)
Cell:normal | Additive 1:AA | Additive 2:pyruvate | Environment:Aerobic | NO status:not specified | Time:not specified (3)
Cell:normal | Additive 1:Glucose | Additive 2:none | Environment:Aerobic | NO status:not specified | Time:not specified (3)
Cell:normal | Additive 1:Glucose | Additive 2:none | Environment:Anaerobic | NO status:NO2 | Time:not specified (3)
Cell:normal | Additive 1:Glucose | Additive 2:none | Environment:Anaerobic | NO status:NO3 | Time:not specified (3)
Cell:normal | Additive 1:Glucose | Additive 2:none | Environment:Anaerobic | NO status:not specified | Time:not specified (3)
Cell:normal | Additive 1:Glucose | Additive 2:none | Environment:Hypoxic | NO status:not specified | Time:not specified (3)
Cell:normal | Additive 1:Glucose | Additive 2:none | Environment:undefined | NO status:NO | Time:0.25 h (3)
Cell:normal | Additive 1:Glucose | Additive 2:none | Environment:undefined | NO status:NO | Time:0.5 h (3)
Cell:normal | Additive 1:Glucose | Additive 2:none | Environment:undefined | NO status:NO | Time:1.5 h (3)
Cell:normal | Additive 1:Glucose | Additive 2:none | Environment:undefined | NO status:NO | Time:1 h (3)
Cell:normal | Additive 1:Glucose | Additive 2:none | Environment:undefined | NO status:NO | Time:2.5 h (3)
Cell:normal | Additive 1:Glucose | Additive 2:none | Environment:undefined | NO status:NO | Time:2 h (3)
Cell:normal | Additive 1:Glucose | Additive 2:none | Environment:undefined | NO status:NO | Time:3 h (3)
Cell:normal | Additive 1:Glucose | Additive 2:none | Environment:undefined | NO status:NO | Time:5 h (3)
Cell:normal | Additive 1:Glucose | Additive 2:pyruvate | Environment:Aerobic | NO status:not specified | Time:not specified (3)

Filtering Parameters
Select threshold % for selection below:
Option 1: No filtering
Option 2: Filter by Sum (Analytes whose sum for all samples falls below the threshold % will be excluded)
Option 3: Filter by Mean (Analytes whose mean for all samples falls below the threshold % will be excluded)
Option 4: Filter by Median (Analytes whose median for all samples falls below the threshold % will be excluded)
Option 5: Filter by Sample threshold       must exceed the above threshold per analyte
In order for an analyte to be retained, the % of samples in one/both groups which are non-zero must exceed the threshold.
Option 6: Remove all analytes containing null values
Sample Normalization Parameters
Normalize samples by:
Volcano Plot Parameters
P-value cutoff: Fold-change cutoff:
MS Search Parameters
Search Refmet database
MS Ion polarity:POSITIVE MS search m/z tolerance:
Ion adducts(choose at least one)
Positive mode:
 [M+H]+ [M+H-H2O]+ [M+Na]+ [M+NH4]+ [M+K]+ [M+2H]2+ [M+2Na]2+ [M+2Na-H]+
[M+H-EtnP]+ [M+H-SerP]+
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