Perform Volcano plot analysis on selected experimental conditions followed by MS search on significant features

Group1Experimental factorGroup2
Age:106 week | Line:HRT | Sex:Female | Training:no (4)
Age:106 week | Line:HRT | Sex:Female | Training:yes (4)
Age:106 week | Line:LRT | Sex:Female | Training:no (4)
Age:106 week | Line:LRT | Sex:Female | Training:yes (4)
Age:126 week | Line:HCR | Sex:Male | Training:no (4)
Age:126 week | Line:LCR | Sex:Male | Training:no (4)
Age:14 week | Line:HCR | Sex:Male | Training:no (5)
Age:14 week | Line:LCR | Sex:Male | Training:no (5)
Age:4 week | Line:HCR | Sex:Male | Training:no (6)
Age:4 week | Line:LCR | Sex:Male | Training:no (6)

Filtering Parameters
Select threshold % for selection below:
Option 1: No filtering
Option 2: Filter by Sum (Analytes whose sum for all samples falls below the threshold % will be excluded)
Option 3: Filter by Mean (Analytes whose mean for all samples falls below the threshold % will be excluded)
Option 4: Filter by Median (Analytes whose median for all samples falls below the threshold % will be excluded)
Option 5: Filter by Sample threshold       must exceed the above threshold per analyte
In order for an analyte to be retained, the % of samples in one/both groups which are non-zero must exceed the threshold.
Option 6: Remove all analytes containing null values
Sample Normalization Parameters
Normalize samples by:
Volcano Plot Parameters
P-value cutoff: Fold-change cutoff:
MS Search Parameters
Search Refmet database
MS Ion polarity:NEGATIVE MS search m/z tolerance:
Ion adducts(choose at least one)
Negative mode:
 [M-H]- [M+Cl]- [M+HCOO]- [M+OAc]- [M-CH3]- [M-2H]2- [M-3H]3- [M-H-Ser]-
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