Perform Volcano plot analysis on selected experimental conditions followed by MS search on significant features

Group1Experimental factorGroup2
Exercise:No | Group:Borderline Pressures | Sex:Female | Type:Cath (18)
Exercise:No | Group:Borderline Pressures | Sex:Male | Type:Cath (3)
Exercise:No | Group:Healthy | Sex:Female | Type:Non-invasive (33)
Exercise:No | Group:Healthy | Sex:Male | Type:Non-invasive (4)
Exercise:No | Group:LowRisk | Sex:Female | Type:Non-invasive (20)
Exercise:No | Group:LowRisk | Sex:Male | Type:Non-invasive (2)
Exercise:No | Group:Normal Pressures | Sex:Female | Type:Cath (9)
Exercise:No | Group:Normal Pressures | Sex:Male | Type:Cath (1)
Exercise:No | Group:PAH | Sex:Female | Type:Cath (17)
Exercise:No | Group:PAH | Sex:Male | Type:Cath (3)
Exercise:Yes | Group:Borderline Pressures | Sex:Female | Type:Cath (18)
Exercise:Yes | Group:Borderline Pressures | Sex:Male | Type:Cath (3)
Exercise:Yes | Group:Healthy | Sex:Female | Type:Non-invasive (33)
Exercise:Yes | Group:Healthy | Sex:Male | Type:Non-invasive (4)
Exercise:Yes | Group:LowRisk | Sex:Female | Type:Non-invasive (20)
Exercise:Yes | Group:LowRisk | Sex:Male | Type:Non-invasive (2)
Exercise:Yes | Group:Normal Pressures | Sex:Female | Type:Cath (9)
Exercise:Yes | Group:Normal Pressures | Sex:Male | Type:Cath (1)
Exercise:Yes | Group:PAH | Sex:Female | Type:Cath (15)
Exercise:Yes | Group:PAH | Sex:Male | Type:Cath (3)

Filtering Parameters
Select threshold % for selection below:
Option 1: No filtering
Option 2: Filter by Sum (Analytes whose sum for all samples falls below the threshold % will be excluded)
Option 3: Filter by Mean (Analytes whose mean for all samples falls below the threshold % will be excluded)
Option 4: Filter by Median (Analytes whose median for all samples falls below the threshold % will be excluded)
Option 5: Filter by Sample threshold       must exceed the above threshold per analyte
In order for an analyte to be retained, the % of samples in one/both groups which are non-zero must exceed the threshold.
Option 6: Remove all analytes containing null values
Sample Normalization Parameters
Normalize samples by:
Volcano Plot Parameters
P-value cutoff: Fold-change cutoff:
MS Search Parameters
Search Refmet database
MS Ion polarity:NEGATIVE MS search m/z tolerance:
Ion adducts(choose at least one)
Negative mode:
[M-H]- [M+Cl]- [M+HCOO]- [M+OAc]- [M-CH3]- [M-2H]2- [M-3H]3- [M-H-Ser]-

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