Log in
/
Register
Home
Overview
Upload / Manage Data
Browse / Search Studies
Analyze Studies
Tutorials
FAQ
Data Repository
Overview
Upload / Manage Studies
Browse / Search Studies
Analyze Studies
Data Sharing Policy
Tutorials
FAQ
Databases
Overview
Metabolite Database
Human Metabolome Gene/Protein Database (MGP)
RefMet: Reference List of Metabolite Names
External Metabolomics Databases (Links)
Protocols
Overview
General Protocols
Study-specific Protocols
Tools
Overview
Load and analyze your own dataset
Analyze Studies
MS Searches
Structure drawing
REST Service
External Tools (Links)
About
Overview
Publishing Partners
How to Cite
How to Link
Terms of Use
Personnel
Contact
Sitemap
Search
Keyword Search
Advanced Searches
Perform Volcano plot analysis on selected experimental conditions followed by MS search on significant features
Group1
Experimental factor
Group2
treatment:Control (27)
treatment:Si p73 (27)
Filtering Parameters
Select threshold % for selection below:
100% (option 4 only)
95%
90%
85%
80%
75%
70%
65%
60%
55%
50%
45%
40%
35%
30%
20%
10%
0%
Option 1:
No filtering
Option 2:
Filter by Sum
(Analytes whose sum for all samples falls below the threshold % will be excluded)
Option 3:
Filter by Mean
(Analytes whose mean for all samples falls below the threshold % will be excluded)
Option 4:
Filter by Median
(Analytes whose median for all samples falls below the threshold % will be excluded)
Option 5:
Filter by Sample threshold
At least one of the 2 selected groups
Both groups
must exceed the above threshold per analyte
In order for an analyte to be retained, the % of samples in one/both groups which are non-zero must exceed the threshold.
Option 6:
Remove all analytes containing null values
Sample Normalization Parameters
Normalize samples by:
None
Sample means
Sample sums
Volcano Plot Parameters
P-value cutoff:
0.1
0.05
0.02
0.01
0.005
0.002
0.001
0.0005
0.0002
Fold-change cutoff:
1.1
1.2
1.25
1.33
1.5
2
3
4
5
MS Search Parameters
Search Refmet database
MS Ion polarity:POSITIVE
MS search m/z tolerance:
0.1
0.05
0.02
0.01
0.005
0.002
0.001
0.0005
0.0002
Feature order:
m/z_rt
rt_m/z
Ion adducts(choose at least one)
Positive mode:
[M+H]+
[M+H-H2O]+
[M+Na]+
[M+NH4]+
[M+K]+
[M+2H]2+
[M+2Na]2+
[M+2Na-H]+
[M+H-EtnP]+
[M+H-SerP]+
Metabolomics Workbench
Terms of use
Site map
Contact