Log in
/
Register
Home
Overview
Upload / Manage Data
Browse / Search Studies
Analyze Studies
Tutorials
FAQ
Data Repository
Overview
Upload / Manage Studies
Browse / Search Studies
Analyze Studies
Data Sharing Policy
Tutorials
FAQ
Databases
Overview
Metabolite Database
Human Metabolome Gene/Protein Database (MGP)
RefMet: Reference List of Metabolite Names
External Metabolomics Databases (Links)
Protocols
Overview
General Protocols
Study-specific Protocols
Tools
Overview
Load and analyze your own dataset
Analyze Studies
MS Searches
Structure drawing
REST Service
External Tools (Links)
About
Overview
Publishing Partners
How to Cite
How to Link
Terms of Use
Personnel
Contact
Sitemap
Search
Keyword Search
Advanced Searches
Perform Volcano plot analysis on selected experimental conditions followed by MS search on significant features
Group1
Experimental factor
Group2
Sample type:alm mix in MQ neg (3)
Sample type:alm mix in MQ pos (3)
Sample type:alm mix in MQ pos (new vial) (1)
Sample type:MQ Blank neg (9)
Sample type:MQ Blank neg (07.31.18 rerun some samples, new aliquot) (1)
Sample type:MQ Blank neg (batch 1) (1)
Sample type:MQ Blank neg (batch 2, 07.30.18) (1)
Sample type:MQ Blank pos (HSST3#5, probe 3, 07.27.18) (1)
Sample type:UW D13B61 neg (1)
Sample type:UW D13B7 neg (1)
Sample type:UW D13B84 neg (1)
Sample type:UW D16B61 neg (1)
Sample type:UW D16B7 neg (1)
Sample type:UW D16B84 neg (1)
Sample type:UW D20B61 neg (2)
Sample type:UW D20B7 neg (2)
Sample type:UW D2B61 neg (2)
Sample type:UW D2B7 neg (2)
Sample type:UW D2B84 neg (1)
Sample type:UW D60B84 neg (1)
Sample type:UW D6B61 neg (1)
Sample type:UW D6B7 neg (1)
Sample type:UW D6B84 neg (1)
Sample type:UW D9B61 neg (1)
Sample type:UW D9B7 neg (1)
Sample type:UW D9B84 neg (1)
Sample type:UW N11B61 neg (1)
Sample type:UW N11B84 neg (1)
Sample type:UW N15B61-EIA neg (1)
Sample type:UW N15B61-EIA neg redo (1)
Sample type:UW N15B84 neg (1)
Sample type:UW N18B61 neg (1)
Sample type:UW N18B84 neg (1)
Sample type:UW N22B61 neg (1)
Sample type:UW N22B7 neg (1)
Sample type:UW N22B84 neg (1)
Sample type:UW N29B7 neg (1)
Sample type:UW N4B61 neg (2)
Sample type:UW N4B7 neg (2)
Sample type:UW N4B84 neg (1)
Sample type:UW N4B89 neg (1)
Sample type:UW N8B61 neg (1)
Sample type:UW N8B7 neg (1)
Sample type:UW O11B61-CTL neg (1)
Sample type:UW O11B61-CTL neg redo (1)
Sample type:UW O11B61 neg (1)
Sample type:UW O11B7-CTL neg (1)
Sample type:UW O11B7-CTL neg redo (1)
Sample type:UW O11B7 neg (1)
Sample type:UW O11B84-NO-CEN neg (1)
Sample type:UW O11B84 neg (1)
Sample type:UW O12B61neg (1)
Sample type:UW O13B61 neg (1)
Sample type:UW O13B7 neg (1)
Sample type:UW O14B61-CTL neg redo (1)
Sample type:UW O14B61 CTL neg (1)
Sample type:UW O14B61 neg (1)
Sample type:UW O14B7-CTL neg (1)
Sample type:UW O14B7-CTL neg redo (1)
Sample type:UW O14B7 neg (1)
Sample type:UW O16B7 neg (1)
Sample type:UW O16B84 neg (1)
Sample type:UW O18B61 neg (2)
Sample type:UW O18B7 neg (2)
Sample type:UW O18B84 CTL neg (1)
Sample type:UW O18B84 neg (1)
Sample type:UW O18B84_2 neg (1)
Sample type:UW O19B61 neg (1)
Sample type:UW O19B7 neg (1)
Sample type:UW O19B84 neg (1)
Sample type:UW O20B61 neg (1)
Sample type:UW O20B7 neg (1)
Sample type:UW O20B84 neg (2)
Sample type:UW O21B61 neg (2)
Sample type:UW O21B7 neg (2)
Sample type:UW O21B84 neg (2)
Sample type:UW O23B61 neg (1)
Sample type:UW O23B7 neg (1)
Sample type:UW O23B84 neg (1)
Sample type:UW O24B61 neg (1)
Sample type:UW O24B84 neg (1)
Sample type:UW O25B61 neg (1)
Sample type:UW O25B84 neg (1)
Sample type:UW O2B84 neg (1)
Sample type:UW O3B61 neg (1)
Sample type:UW O3B7 neg (1)
Sample type:UW O3B84 neg (1)
Sample type:UW O4B61 neg (2)
Sample type:UW O4B7 neg (2)
Sample type:UW O4B84 neg (2)
Sample type:UW O5B61 neg (1)
Sample type:UW O5B7 neg (1)
Sample type:UW O5B84 neg (1)
Sample type:UW O6B61 neg (1)
Sample type:UW O6B7 neg (1)
Sample type:UW O6B84 neg (1)
Sample type:UW pooled neg (22)
Sample type:UW pooled neg condition (17)
Sample type:UW pooled pos condition (1)
Sample type:UW pooled with spike neg (3)
Filtering Parameters
Select threshold % for selection below:
100% (option 4 only)
95%
90%
85%
80%
75%
70%
65%
60%
55%
50%
45%
40%
35%
30%
20%
10%
0%
Option 1:
No filtering
Option 2:
Filter by Sum
(Analytes whose sum for all samples falls below the threshold % will be excluded)
Option 3:
Filter by Mean
(Analytes whose mean for all samples falls below the threshold % will be excluded)
Option 4:
Filter by Median
(Analytes whose median for all samples falls below the threshold % will be excluded)
Option 5:
Filter by Sample threshold
At least one of the 2 selected groups
Both groups
must exceed the above threshold per analyte
In order for an analyte to be retained, the % of samples in one/both groups which are non-zero must exceed the threshold.
Option 6:
Remove all analytes containing null values
Sample Normalization Parameters
Normalize samples by:
None
Sample means
Sample sums
Volcano Plot Parameters
P-value cutoff:
0.1
0.05
0.02
0.01
0.005
0.002
0.001
0.0005
0.0002
Fold-change cutoff:
1.1
1.2
1.25
1.33
1.5
2
3
4
5
MS Search Parameters
Search Refmet database
MS Ion polarity:NEGATIVE
MS search m/z tolerance:
0.1
0.05
0.02
0.01
0.005
0.002
0.001
0.0005
0.0002
Feature order:
m/z_rt
rt_m/z
Ion adducts(choose at least one)
Negative mode:
[M-H]-
[M+Cl]-
[M+HCOO]-
[M+OAc]-
[M-CH3]-
[M-2H]2-
[M-3H]3-
[M-H-Ser]-
Metabolomics Workbench
Terms of use
Site map
Contact