Perform Volcano plot analysis on selected experimental conditions followed by MS search on significant features

Group1Experimental factorGroup2
Site:Bangladesh | GA_delivery:24 | GA_sampling:17 (2)
Site:Bangladesh | GA_delivery:29 | GA_sampling:13 (2)
Site:Bangladesh | GA_delivery:29 | GA_sampling:15 (4)
Site:Bangladesh | GA_delivery:30 | GA_sampling:13 (2)
Site:Bangladesh | GA_delivery:31 | GA_sampling:11 (2)
Site:Bangladesh | GA_delivery:31 | GA_sampling:15 (2)
Site:Bangladesh | GA_delivery:31 | GA_sampling:16 (2)
Site:Bangladesh | GA_delivery:31 | GA_sampling:8 (2)
Site:Bangladesh | GA_delivery:33 | GA_sampling:17 (2)
Site:Bangladesh | GA_delivery:41 | GA_sampling:11 (2)
Site:Bangladesh | GA_delivery:41 | GA_sampling:13 (4)
Site:Bangladesh | GA_delivery:41 | GA_sampling:15 (2)
Site:Bangladesh | GA_delivery:41 | GA_sampling:16 (6)
Site:Bangladesh | GA_delivery:41 | GA_sampling:17 (2)
Site:Bangladesh | GA_delivery:41 | GA_sampling:18 (2)
Site:Bangladesh | GA_delivery:41 | GA_sampling:8 (2)
Site:Bangladesh_GAPPS | GA_delivery:29 | GA_sampling:12 (1)
Site:Bangladesh_GAPPS | GA_delivery:32 | GA_sampling:11 (1)
Site:Bangladesh_GAPPS | GA_delivery:33 | GA_sampling:12 (1)
Site:Bangladesh_GAPPS | GA_delivery:33 | GA_sampling:13 (2)
Site:Bangladesh_GAPPS | GA_delivery:33 | GA_sampling:15 (1)
Site:Bangladesh_GAPPS | GA_delivery:34 | GA_sampling:11 (1)
Site:Bangladesh_GAPPS | GA_delivery:35 | GA_sampling:11 (1)
Site:Bangladesh_GAPPS | GA_delivery:36 | GA_sampling:11 (2)
Site:Bangladesh_GAPPS | GA_delivery:39 | GA_sampling:13 (1)
Site:Bangladesh_GAPPS | GA_delivery:39 | GA_sampling:19 (1)
Site:Bangladesh_GAPPS | GA_delivery:40 | GA_sampling:11 (2)
Site:Bangladesh_GAPPS | GA_delivery:40 | GA_sampling:12 (6)
Site:Pakistan | GA_delivery:28 | GA_sampling:9 (2)
Site:Pakistan | GA_delivery:32 | GA_sampling:17 (2)
Site:Pakistan | GA_delivery:32 | GA_sampling:8 (2)
Site:Pakistan | GA_delivery:32 | GA_sampling:9 (2)
Site:Pakistan | GA_delivery:33 | GA_sampling:10 (2)
Site:Pakistan | GA_delivery:33 | GA_sampling:12 (2)
Site:Pakistan | GA_delivery:33 | GA_sampling:16 (2)
Site:Pakistan | GA_delivery:33 | GA_sampling:17 (2)
Site:Pakistan | GA_delivery:33 | GA_sampling:18 (4)
Site:Pakistan | GA_delivery:39 | GA_sampling:10 (2)
Site:Pakistan | GA_delivery:39 | GA_sampling:12 (2)
Site:Pakistan | GA_delivery:39 | GA_sampling:17 (2)
Site:Pakistan | GA_delivery:39 | GA_sampling:18 (2)
Site:Pakistan | GA_delivery:39 | GA_sampling:8 (2)
Site:Pakistan | GA_delivery:39 | GA_sampling:9 (4)
Site:Pakistan | GA_delivery:40 | GA_sampling:16 (2)
Site:Pakistan | GA_delivery:40 | GA_sampling:17 (2)
Site:Pakistan | GA_delivery:40 | GA_sampling:18 (2)
Site:Tanzania | GA_delivery:26 | GA_sampling:11 (2)
Site:Tanzania | GA_delivery:26 | GA_sampling:12 (2)
Site:Tanzania | GA_delivery:26 | GA_sampling:19 (2)
Site:Tanzania | GA_delivery:31 | GA_sampling:16 (2)
Site:Tanzania | GA_delivery:32 | GA_sampling:10 (2)
Site:Tanzania | GA_delivery:32 | GA_sampling:13 (2)
Site:Tanzania | GA_delivery:32 | GA_sampling:9 (2)
Site:Tanzania | GA_delivery:33 | GA_sampling:14 (2)
Site:Tanzania | GA_delivery:33 | GA_sampling:15 (2)
Site:Tanzania | GA_delivery:33 | GA_sampling:18 (2)
Site:Tanzania | GA_delivery:39 | GA_sampling:10 (2)
Site:Tanzania | GA_delivery:39 | GA_sampling:11 (2)
Site:Tanzania | GA_delivery:39 | GA_sampling:12 (2)
Site:Tanzania | GA_delivery:39 | GA_sampling:13 (2)
Site:Tanzania | GA_delivery:39 | GA_sampling:14 (2)
Site:Tanzania | GA_delivery:39 | GA_sampling:15 (2)
Site:Tanzania | GA_delivery:39 | GA_sampling:16 (2)
Site:Tanzania | GA_delivery:39 | GA_sampling:18 (2)
Site:Tanzania | GA_delivery:39 | GA_sampling:19 (2)
Site:Tanzania | GA_delivery:39 | GA_sampling:9 (2)
Site:Zambia_GAPPS | GA_delivery:28 | GA_sampling:17 (1)
Site:Zambia_GAPPS | GA_delivery:28 | GA_sampling:8 (4)
Site:Zambia_GAPPS | GA_delivery:31 | GA_sampling:11 (2)
Site:Zambia_GAPPS | GA_delivery:31 | GA_sampling:19 (2)
Site:Zambia_GAPPS | GA_delivery:32 | GA_sampling:17 (1)
Site:Zambia_GAPPS | GA_delivery:33 | GA_sampling:13 (2)
Site:Zambia_GAPPS | GA_delivery:33 | GA_sampling:17 (1)
Site:Zambia_GAPPS | GA_delivery:35 | GA_sampling:19 (1)
Site:Zambia_GAPPS | GA_delivery:36 | GA_sampling:16 (2)
Site:Zambia_GAPPS | GA_delivery:37 | GA_sampling:16 (2)
Site:Zambia_GAPPS | GA_delivery:40 | GA_sampling:10 (1)
Site:Zambia_GAPPS | GA_delivery:40 | GA_sampling:14 (1)
Site:Zambia_GAPPS | GA_delivery:40 | GA_sampling:15 (1)
Site:Zambia_GAPPS | GA_delivery:40 | GA_sampling:17 (3)
Site:Zambia_GAPPS | GA_delivery:40 | GA_sampling:18 (1)
Site:Zambia_GAPPS | GA_delivery:40 | GA_sampling:19 (3)
Site:Zambia_GAPPS | GA_delivery:41 | GA_sampling:18 (4)

Filtering Parameters
Select threshold % for selection below:
Option 1: No filtering
Option 2: Filter by Sum (Analytes whose sum for all samples falls below the threshold % will be excluded)
Option 3: Filter by Mean (Analytes whose mean for all samples falls below the threshold % will be excluded)
Option 4: Filter by Median (Analytes whose median for all samples falls below the threshold % will be excluded)
Option 5: Filter by Sample threshold       must exceed the above threshold per analyte
In order for an analyte to be retained, the % of samples in one/both groups which are non-zero must exceed the threshold.
Option 6: Remove all analytes containing null values
Sample Normalization Parameters
Normalize samples by:
Volcano Plot Parameters
P-value cutoff: Fold-change cutoff:
MS Search Parameters
Search Refmet database
MS Ion polarity:POSITIVE MS search m/z tolerance:
Ion adducts(choose at least one)
Positive mode:
 [M+H]+ [M+H-H2O]+ [M+Na]+ [M+NH4]+ [M+K]+ [M+2H]2+ [M+2Na]2+ [M+2Na-H]+
[M+H-EtnP]+ [M+H-SerP]+
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