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MGP004556

UniProt Annotations

Entry Information
Gene Nameninein-like
Protein EntryNINL_HUMAN
UniProt IDQ9Y2I6
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=1; Synonyms=A; IsoId=Q9Y2I6-1; Sequence=Displayed; Name=2; Synonyms=B; IsoId=Q9Y2I6-2; Sequence=VSP_037883;
DomainThe KEN and D (destructive) boxes are required for the cell cycle-controlled NINL degradation by the APC/C pathway. {ECO:0000269|PubMed:17403670}.
FunctionInvolved in the microtubule organization in interphase cells. Overexpression induces the fragmentation of the Golgi, and causes lysosomes to disperse toward the cell periphery; it also interferes with mitotic spindle assembly. May play a role in ovarian carcinogenesis. {ECO:0000269|PubMed:12852856, ECO:0000269|PubMed:16254247, ECO:0000269|PubMed:18538832}.
PtmPhosphorylated by PLK1 which disrupts its centrosome association and interaction with gamma-tubulin. {ECO:0000269|PubMed:12852856, ECO:0000269|PubMed:16254247}.
PtmUbiquitinated by the APC/C complex leading to its degradation. {ECO:0000269|PubMed:17403670}.
Sequence CautionSequence=BAA76824.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAH11644.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=BAH11644.1; Type=Erroneous termination; Positions=405; Note=Translated as Gln.; Evidence={ECO:0000305};
SimilarityContains 4 EF-hand domains. {ECO:0000255|PROSITE- ProRule:PRU00448}.
Subcellular LocationCytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm. Note=In interphase cells, NINL is transported to the centrosomes by the dynein- dynactin motor complex. During centrosome maturation, PLK1 directly phosphorylates NINL resulting in its release into the cytoplasm.
SubunitInteracts with gamma-tubulin and TUBGCP4. Interacts with anaphase promoting complex/cyclosome (APC/C). Interacts with CDC20 and FZR1. Isoform 2 interacts with LCA5 and USH2A. {ECO:0000269|PubMed:12852856, ECO:0000269|PubMed:17403670, ECO:0000269|PubMed:18826961}.
Tissue SpecificityExpressed in KYSE-150 esophageal carcinoma, HeLa cervical carcinoma and U2OS osteosarcoma cells. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase (at protein level). Expressed in fetal heart, skeletal muscle, liver, lung and cochlea, and in adult brain, testis, kidney and retina. {ECO:0000269|PubMed:17403670, ECO:0000269|PubMed:18826961}.
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