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MB Sample ID: SA206876
| Local Sample ID: | C9 |
| Subject ID: | SU002242 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002242 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| C9 | SA206876 | FL025308 | High-fat diet | raw_data_time_format |
Collection:
| Collection ID: | CO002235 |
| Collection Summary: | Liver biopsies from experimental mice were collected and immediately frozen in liquid nitrogen. |
| Sample Type: | Liver |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR002254 |
| Treatment Summary: | Mice were treated with a normal diet, high-fat high-sugar diet, or treated with a high-fat high-sugar diet and an antibiotic cocktail (ABX). |
Sample Preparation:
| Sampleprep ID: | SP002248 |
| Sampleprep Summary: | Briefly, liver tissues (50-100 mg) are typically homogenized in the extraction solvent using a bead beater. These samples are then centrifuged at 13,000 g for 15 min to pellet tissue and proteins. The supernatant is combined with water and chloroform, including internal standards, to separate into the polar and non-polar phases. |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
Combined analysis:
| Analysis ID | AN003531 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890N |
| Column | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| MS Type | EI |
| MS instrument type | Ion trap |
| MS instrument name | Agilent 5973 |
| Ion Mode | POSITIVE |
| Units | None |
Chromatography:
| Chromatography ID: | CH002608 |
| Chromatography Summary: | GC conditions included GC System (Agilent 6890) with column (DB-5MS, 60 m, 0.25 mm ID, 0.25 µm film thickness, J&W Scientific), and gas type helium. MS conditions included MS system (Agilent 5973 MSD) with electron ionization (EI ionization) with scan range from m/z 50 to m/z 650. |
| Instrument Name: | Agilent 6890N |
| Column Name: | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS003289 |
| Analysis ID: | AN003531 |
| Instrument Name: | Agilent 5973 |
| Instrument Type: | Ion trap |
| MS Type: | EI |
| MS Comments: | The data are deconvoluted using AMDIS and annotated using an in-house constructed spectral library |
| Ion Mode: | POSITIVE |
