Summary of Study ST002156
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001369. The data can be accessed directly via it's Project DOI: 10.21228/M8RQ67 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002156 |
| Study Title | Mouse liver metabolites |
| Study Summary | Western diet (WD) and gut microbiota interplay drives the development of non-alcoholic fatty liver disease (NAFLD) and its progression to non-alcoholic steatohepatitis (NASH). However, the metabolic mediators contributing to NASH remain to be identified. In this study, we explored the change of liver metabolites in a diet-induced mouse NASH model. Identification of these metabolites will help to uncover the pathogenesis of NASH and explore the new therapeutic treatment. |
| Institute | University of Missouri-Columbia |
| Last Name | Yang |
| First Name | Ming |
| Address | University of Missouri |
| yangmin@health.missouri.edu | |
| Phone | 5738827141 |
| Submit Date | 2022-04-24 |
| Analysis Type Detail | GC-MS |
| Release Date | 2022-05-16 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001369 |
| Project DOI: | doi: 10.21228/M8RQ67 |
| Project Title: | Contribution of western diet to NASH pathogenesis by defining liver metabolites |
| Project Summary: | Western diet (WD) and gut microbiota interplay drives the development of non-alcoholic fatty liver disease (NAFLD) and its progression to non-alcoholic steatohepatitis (NASH). However, the metabolic mediators contributing to NASH remain to be identified. In this study, we explored the change of liver metabolites in a diet-induced mouse NASH model. Identification of these metabolites will help to uncover the pathogenesis of NASH and explore the new therapeutic treatment. |
| Institute: | University of Missouri-Columbia |
| Last Name: | Yang |
| First Name: | Ming |
| Address: | University of Missouri, NextGen Prevision Health Building, Room 2203 |
| Email: | yangmin@health.missouri.edu |
| Phone: | 573-882-7141 |
Subject:
| Subject ID: | SU002242 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | raw_data_time_format |
|---|---|---|
| SA206875 | C1 | High-fat diet |
| SA206876 | C9 | High-fat diet |
| SA206877 | C10 | High-fat diet |
| SA206878 | C7 | High-fat diet |
| SA206879 | C8 | High-fat diet |
| SA206880 | C6 | High-fat diet |
| SA206881 | C3 | High-fat diet |
| SA206882 | C2 | High-fat diet |
| SA206883 | C4 | High-fat diet |
| SA206884 | C5 | High-fat diet |
| SA206885 | A7 | High-fat diet + Antibiotic cocktail |
| SA206886 | A8 | High-fat diet + Antibiotic cocktail |
| SA206887 | A10 | High-fat diet + Antibiotic cocktail |
| SA206888 | A9 | High-fat diet + Antibiotic cocktail |
| SA206889 | A6 | High-fat diet + Antibiotic cocktail |
| SA206890 | A5 | High-fat diet + Antibiotic cocktail |
| SA206891 | A2 | High-fat diet + Antibiotic cocktail |
| SA206892 | A1 | High-fat diet + Antibiotic cocktail |
| SA206893 | A4 | High-fat diet + Antibiotic cocktail |
| SA206894 | A3 | High-fat diet + Antibiotic cocktail |
| SA206895 | N8 | ND |
| SA206896 | N10 | ND |
| SA206897 | N7 | ND |
| SA206898 | N9 | ND |
| SA206899 | N4 | ND |
| SA206900 | N1 | ND |
| SA206901 | N2 | ND |
| SA206902 | N3 | ND |
| SA206903 | N5 | ND |
| SA206904 | N6 | ND |
| Showing results 1 to 30 of 30 |
Collection:
| Collection ID: | CO002235 |
| Collection Summary: | Liver biopsies from experimental mice were collected and immediately frozen in liquid nitrogen. |
| Sample Type: | Liver |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR002254 |
| Treatment Summary: | Mice were treated with a normal diet, high-fat high-sugar diet, or treated with a high-fat high-sugar diet and an antibiotic cocktail (ABX). |
Sample Preparation:
| Sampleprep ID: | SP002248 |
| Sampleprep Summary: | Briefly, liver tissues (50-100 mg) are typically homogenized in the extraction solvent using a bead beater. These samples are then centrifuged at 13,000 g for 15 min to pellet tissue and proteins. The supernatant is combined with water and chloroform, including internal standards, to separate into the polar and non-polar phases. |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
Combined analysis:
| Analysis ID | AN003531 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890N |
| Column | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| MS Type | EI |
| MS instrument type | Ion trap |
| MS instrument name | Agilent 5973 |
| Ion Mode | POSITIVE |
| Units | None |
Chromatography:
| Chromatography ID: | CH002608 |
| Chromatography Summary: | GC conditions included GC System (Agilent 6890) with column (DB-5MS, 60 m, 0.25 mm ID, 0.25 µm film thickness, J&W Scientific), and gas type helium. MS conditions included MS system (Agilent 5973 MSD) with electron ionization (EI ionization) with scan range from m/z 50 to m/z 650. |
| Instrument Name: | Agilent 6890N |
| Column Name: | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS003289 |
| Analysis ID: | AN003531 |
| Instrument Name: | Agilent 5973 |
| Instrument Type: | Ion trap |
| MS Type: | EI |
| MS Comments: | The data are deconvoluted using AMDIS and annotated using an in-house constructed spectral library |
| Ion Mode: | POSITIVE |
