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MB Sample ID: SA206897
Local Sample ID: | N7 |
Subject ID: | SU002242 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Male |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002242 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Male |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
N7 | SA206897 | FL025310 | ND | raw_data_time_format |
Collection:
Collection ID: | CO002235 |
Collection Summary: | Liver biopsies from experimental mice were collected and immediately frozen in liquid nitrogen. |
Sample Type: | Liver |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR002254 |
Treatment Summary: | Mice were treated with a normal diet, high-fat high-sugar diet, or treated with a high-fat high-sugar diet and an antibiotic cocktail (ABX). |
Sample Preparation:
Sampleprep ID: | SP002248 |
Sampleprep Summary: | Briefly, liver tissues (50-100 mg) are typically homogenized in the extraction solvent using a bead beater. These samples are then centrifuged at 13,000 g for 15 min to pellet tissue and proteins. The supernatant is combined with water and chloroform, including internal standards, to separate into the polar and non-polar phases. |
Processing Storage Conditions: | -80℃ |
Extract Storage: | -80℃ |
Combined analysis:
Analysis ID | AN003531 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 6890N |
Column | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
MS Type | EI |
MS instrument type | Single quadrupole |
MS instrument name | Agilent 5973 |
Ion Mode | POSITIVE |
Units | None |
Chromatography:
Chromatography ID: | CH002608 |
Chromatography Summary: | GC conditions included GC System (Agilent 6890) with column (DB-5MS, 60 m, 0.25 mm ID, 0.25 µm film thickness, J&W Scientific), and gas type helium. MS conditions included MS system (Agilent 5973 MSD) with electron ionization (EI ionization) with scan range from m/z 50 to m/z 650. |
Instrument Name: | Agilent 6890N |
Column Name: | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
Chromatography Type: | GC |
MS:
MS ID: | MS003289 |
Analysis ID: | AN003531 |
Instrument Name: | Agilent 5973 |
Instrument Type: | Single quadrupole |
MS Type: | EI |
MS Comments: | The data are deconvoluted using AMDIS and annotated using an in-house constructed spectral library |
Ion Mode: | POSITIVE |