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MB Sample ID: SA216237
| Local Sample ID: | 26_019 |
| Subject ID: | SU002335 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Gender: | Male and female |
| Animal Light Cycle: | 12:12 |
| Animal Feed: | 60% high-fat diet (Research Diets Cat# D12492) |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002335 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Gender: | Male and female |
| Animal Light Cycle: | 12:12 |
| Animal Feed: | 60% high-fat diet (Research Diets Cat# D12492) |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 26_019 | SA216237 | FL026089 | Female | Sex |
| 26_019 | SA216237 | FL026089 | Saline | Treatment |
Collection:
| Collection ID: | CO002328 |
| Collection Summary: | Mice were euthanized and livers collected and frozen in liquid N2. |
| Sample Type: | Liver |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR002347 |
| Treatment Summary: | Male and female C57BL/6J mice were placed on 60% high-fat diet (Research Diets, Cat# D12492) for approx. 10 weeks beginning at 10 weeks of age. Mice were then counterbalanced into two treatment groups based on body weight (n = 9-11 mice/ sex/ treatment). For experiment, one dose of FGF21 (0.1 mg/kg, ip) or saline vehicle was given right before the onset of the dark cycle, then another dose (0.1 mg/kg, ip) was given the following morning after the onset of the light cycle. Mice were euthanized two hours following the AM injection, livers collected, and frozen in liquid N2. |
| Treatment Compound: | recombinant human FGF21 (ProSpecBio, Cat# CYT-474) |
| Treatment Route: | intraperitoneal injection |
| Treatment Dose: | 0.1 mg/kg |
| Treatment Dosevolume: | 0.01 mg/mL |
| Treatment Vehicle: | saline |
Sample Preparation:
| Sampleprep ID: | SP002341 |
| Sampleprep Summary: | See attached file. |
| Sampleprep Protocol ID: | methods_sampleprep.pdf |
| Sampleprep Protocol Filename: | methods_sampleprep.pdf |
Combined analysis:
| Analysis ID | AN003675 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890A |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Agilent 7890A |
| Ion Mode | POSITIVE |
| Units | peak |
Chromatography:
| Chromatography ID: | CH002725 |
| Methods Filename: | methods_GC.pdf |
| Instrument Name: | Agilent 7890A |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS003426 |
| Analysis ID: | AN003675 |
| Instrument Name: | Agilent 7890A |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| MS Comments: | See attached file. |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | methods_dataprocessing.pdf |
