Summary of Study ST002249

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001437. The data can be accessed directly via it's Project DOI: 10.21228/M8ZX31 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002249
Study TitleSex-dependent effects of FGF21 on hepatic metabolism
Study SummaryTo investigate sex-dependent effects of fibroblast growth factor-21 (FGF21), diet-induced obese male and female wildtype mice were administered two acute doses of FGF21 or saline vehicle. Mice were euthanized, livers collected, and submitted for untargeted metabolomics analysis.
Institute
University of California, Davis
DepartmentNeurobiology, Physiology and Behavior
LaboratoryRyan
Last NameChaffin
First NameAki
Address1 Shields Ave
Emailatchaffin@ucdavis.edu
Phone603-359-1907
Submit Date2022-08-09
Num Groups4
Total Subjects41
Num Males22
Num Females19
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2022-08-22
Release Version1
Aki Chaffin Aki Chaffin
https://dx.doi.org/10.21228/M8ZX31
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001437
Project DOI:doi: 10.21228/M8ZX31
Project Title:Sex-dependent effects of FGF21 on hepatic metabolism
Project Summary:Untargeted metabolomics analysis of livers from male and female mice administered FGF21 or saline vehicle.
Institute:University of California, Davis
Last Name:Chaffin
First Name:Aki
Address:1 Shields Ave
Email:atchaffin@ucdavis.edu
Phone:603-359-1907
Publications:https://doi.org/10.1172/jci.insight.155848

Subject:

Subject ID:SU002335
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Gender:Male and female
Animal Light Cycle:12:12
Animal Feed:60% high-fat diet (Research Diets Cat# D12492)

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sex Treatment
SA21622234_028Female FGF21
SA21622330_020Female FGF21
SA21622427_004Female FGF21
SA21622537_036Female FGF21
SA21622638_012Female FGF21
SA21622741_032Female FGF21
SA21622840_008Female FGF21
SA21622923_016Female FGF21
SA21623028_024Female FGF21
SA21623131_003Female Saline
SA21623229_011Female Saline
SA21623333_015Female Saline
SA21623435_031Female Saline
SA21623539_007Female Saline
SA21623636_027Female Saline
SA21623726_019Female Saline
SA21623832_039Female Saline
SA21623925_035Female Saline
SA21624024_023Female Saline
SA21624121_002Male FGF21
SA2162427_006Male FGF21
SA21624314_038Male FGF21
SA2162448_014Male FGF21
SA21624513_041Male FGF21
SA21624622_026Male FGF21
SA2162471_034Male FGF21
SA21624817_010Male FGF21
SA21624910_022Male FGF21
SA2162503_030Male FGF21
SA21625118_018Male FGF21
SA2162525_021Male Saline
SA2162536_033Male Saline
SA2162549_001Male Saline
SA21625511_025Male Saline
SA21625620_005Male Saline
SA21625719_040Male Saline
SA21625816_029Male Saline
SA21625915_009Male Saline
SA2162602_037Male Saline
SA2162614_017Male Saline
SA21626212_013Male Saline
Showing results 1 to 41 of 41

Collection:

Collection ID:CO002328
Collection Summary:Mice were euthanized and livers collected and frozen in liquid N2.
Sample Type:Liver
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002347
Treatment Summary:Male and female C57BL/6J mice were placed on 60% high-fat diet (Research Diets, Cat# D12492) for approx. 10 weeks beginning at 10 weeks of age. Mice were then counterbalanced into two treatment groups based on body weight (n = 9-11 mice/ sex/ treatment). For experiment, one dose of FGF21 (0.1 mg/kg, ip) or saline vehicle was given right before the onset of the dark cycle, then another dose (0.1 mg/kg, ip) was given the following morning after the onset of the light cycle. Mice were euthanized two hours following the AM injection, livers collected, and frozen in liquid N2.
Treatment Compound:recombinant human FGF21 (ProSpecBio, Cat# CYT-474)
Treatment Route:intraperitoneal injection
Treatment Dose:0.1 mg/kg
Treatment Dosevolume:0.01 mg/mL
Treatment Vehicle:saline

Sample Preparation:

Sampleprep ID:SP002341
Sampleprep Summary:See attached file.
Sampleprep Protocol ID:methods_sampleprep.pdf
Sampleprep Protocol Filename:methods_sampleprep.pdf

Combined analysis:

Analysis ID AN003675
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890A
Column Restek Rtx-5Sil (30m x 0.25mm,0.25um)
MS Type EI
MS instrument type GC-TOF
MS instrument name Agilent 7890A
Ion Mode POSITIVE
Units peak

Chromatography:

Chromatography ID:CH002725
Methods Filename:methods_GC.pdf
Instrument Name:Agilent 7890A
Column Name:Restek Rtx-5Sil (30m x 0.25mm,0.25um)
Chromatography Type:GC

MS:

MS ID:MS003426
Analysis ID:AN003675
Instrument Name:Agilent 7890A
Instrument Type:GC-TOF
MS Type:EI
MS Comments:See attached file.
Ion Mode:POSITIVE
Analysis Protocol File:methods_dataprocessing.pdf
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