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MB Sample ID: SA005498
Local Sample ID: | HSP_F9_pos.raw |
Subject ID: | SU000117 |
Subject Type: | Invertebrate |
Subject Species: | Caenorhabditis elegans |
Taxonomy ID: | 6239 |
Genotype Strain: | N2 |
Species Group: | Invertebrate |
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Analysis:
MB Sample ID: | SA005498 |
Analysis ID: | AN000161 |
Laboratory Name: | Arthur Edison |
Analysis Type: | NMR |
Analysis Comments: | HSQC-TOCSY dataset was collected using the custom 1.5 mm HTS prob |
Software Version: | VNMRJ |
Operator Name: | Chaevien Clendinen |
Randomization Order: | NMR/Processed |
Data Format: | Varian FID |
Chromatography ID: | CH000112 |
Num Factors: | 3 |
MB Sample ID: | SA005498 |
Analysis ID: | AN000162 |
Laboratory Name: | Arthur Edison |
Analysis Type: | NMR |
Analysis Comments: | Carbon 1D dataset was all collected using the custom 1.5 mm HTS prob |
Software Version: | VNMRJ |
Operator Name: | Chaevien Clendinen |
Randomization Order: | NMR/Processed |
Data Format: | Varian FID |
Chromatography ID: | CH000112 |
Num Factors: | 3 |
MB Sample ID: | SA005498 |
Analysis ID: | AN000158 |
Laboratory Name: | Timothy Garrett |
Analysis Type: | MS |
Analysis Comments: | Samples were analyzed using a mass range of 70-1000 in positive and negative ionization modes, externally calibrated, using a Thermo Scientific Q-Exactive Orbitrap mass spectrometer equipped with a Dionex UltiMate 3000 RS autosampler and pump. The Q-Exactive was equipped with a Heated Electrospray Ionization (HESI) source which operated at a spray temperature of 350 ?C, a spray voltage of 3.5 kV, and sheath and auxiliary gas flow rates of 50 and 10 arbitrary units, respectively. The capillary temperature was held at 325 ?C, and the S-lens RF Level was set to 40%. The FR resolution was set to 70,000 at m/z 200. The accuracy achieved was routinely less than 1.5 ppm. In addition to full MS, data-dependent MS/MS was performed on the 10 most abundance peaks with an exclusion period of 10 seconds (40 normalized collision energy). Fractionation was performed using a Dionex UltiMate 3000 fraction collector by triggering every 30 seconds. |
Instrument Name: | Thermo Scientific Q-Exactive |
Operator Name: | Timothy Garrett |
Detector Type: | Thermo Scientific Q-Exactive Orbitrap/MS2 Positive |
Chromatography ID: | CH000112 |
Num Factors: | 3 |
MB Sample ID: | SA005498 |
Analysis ID: | AN000160 |
Laboratory Name: | Arthur Edison |
Analysis Type: | NMR |
Analysis Comments: | HSQC dataset was collected using the custom 1.5 mm HTS probe |
Software Version: | VNMRJ |
Operator Name: | Chaevien Clendinen |
Randomization Order: | NMR/Processed |
Data Format: | Varian FID |
Chromatography ID: | CH000112 |
Num Factors: | 3 |
MB Sample ID: | SA005498 |
Analysis ID: | AN000159 |
Laboratory Name: | Timothy Garrett |
Analysis Type: | MS |
Analysis Comments: | Samples were analyzed using a mass range of 70-1000 in positive and negative ionization modes, externally calibrated, using a Thermo Scientific Q-Exactive Orbitrap mass spectrometer equipped with a Dionex UltiMate 3000 RS autosampler and pump. The Q-Exactive was equipped with a Heated Electrospray Ionization (HESI) source which operated at a spray temperature of 350 ?C, a spray voltage of 3.5 kV, and sheath and auxiliary gas flow rates of 50 and 10 arbitrary units, respectively. The capillary temperature was held at 325 ?C, and the S-lens RF Level was set to 40%. The FR resolution was set to 70,000 at m/z 200. The accuracy achieved was routinely less than 1.5 ppm. In addition to full MS, data-dependent MS/MS was performed on the 10 most abundance peaks with an exclusion period of 10 seconds (40 normalized collision energy). Fractionation was performed using a Dionex UltiMate 3000 fraction collector by triggering every 30 seconds. |
Instrument Name: | Thermo Scientific Q-Exactive |
Operator Name: | Timothy Garrett |
Detector Type: | Thermo Scientific Q-Exactive Orbitrap/MS2 Negative |
Chromatography ID: | CH000112 |
Num Factors: | 3 |
MB Sample ID: | SA005498 |
Analysis ID: | AN000157 |
Laboratory Name: | Timothy Garrett |
Analysis Type: | MS |
Analysis Comments: | Samples were analyzed using a mass range of 70-1000 in positive and negative ionization modes, externally calibrated, using a Thermo Scientific Q-Exactive Orbitrap mass spectrometer equipped with a Dionex UltiMate 3000 RS autosampler and pump. The Q-Exactive was equipped with a Heated Electrospray Ionization (HESI) source which operated at a spray temperature of 350 ?C, a spray voltage of 3.5 kV, and sheath and auxiliary gas flow rates of 50 and 10 arbitrary units, respectively. The capillary temperature was held at 325 ?C, and the S-lens RF Level was set to 40%. The FR resolution was set to 70,000 at m/z 200. The accuracy achieved was routinely less than 1.5 ppm. In addition to full MS, data-dependent MS/MS was performed on the 10 most abundance peaks with an exclusion period of 10 seconds (40 normalized collision energy). Fractionation was performed using a Dionex UltiMate 3000 fraction collector by triggering every 30 seconds. |
Instrument Name: | Thermo Scientific Q-Exactive |
Operator Name: | Timothy Garrett |
Detector Type: | Thermo Scientific Q-Exactive Orbitrap/MS Positive |
Chromatography ID: | CH000112 |
Num Factors: | 3 |