Summary of Study ST000215
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000169. The data can be accessed directly via it's Project DOI: 10.21228/M8N88K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000215 |
Study Title | Liver and Plasma metaboites for 13C-glucose load in wild type, LIRKO GTT 1 mice |
Study Type | 13C mass isotopomer analysis (flux studies) |
Study Summary | Mice were fasted for 18 hr overnight then sacrificed or treated with 13C-U-glucose (2 g/kg ip) and sacrificed 1 hr later by decapitation and liver was immediately freeze-clamped and stored in liquid N2 and then at -80 C. Wild type (IR and IR/FoxO1 floxed) mice were sacrificed after fasting and 1 hr post-glucose treatment. Liver-specific insulin receptor knockout (LIRKO) and insulin receptor/FoxO1 double knockout (LIRFKO) mice were sacrificed 1 hr post glucose treatment. http://www.nature.com/ncomms/2015/150512/ncomms8079/full/ncomms8079.html |
Institute | University of Michigan |
Department | Biomedical Research Core Facilities |
Laboratory | Metabolomics core |
Last Name | Kachman |
First Name | Maureen |
Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
mkachman@umich.edu | |
Submit Date | 2015-06-12 |
Num Groups | 5 |
Total Subjects | 40 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Uploaded File Size | 2.5 G |
Analysis Type Detail | GC-MS/LC-MS |
Release Date | 2015-12-28 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000317 | AN000318 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | GC |
Chromatography system | Agilent 1260 | Agilent 7890A |
Column | Phenomenex Luna NH2 (150 x 1mm,3um) | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
MS Type | ESI | EI |
MS instrument type | QTOF | Single quadrupole |
MS instrument name | Agilent 6520 QTOF | Agilent 5975C |
Ion Mode | NEGATIVE | POSITIVE |
Units | Area | Area |