Summary of Study ST001398
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000958. The data can be accessed directly via it's Project DOI: 10.21228/M8VM30 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001398 |
Study Title | Mechanism of Trichloroethylene (TCE) toxicity in the placenta |
Study Type | MS analysis |
Study Summary | Cells in culture will be exposed to S-(1,2-dichlorovinyl)-L-cysteine (DCVC), a relevant metabolite of TCE. The first cell line used will be HTR-8/SVneo, originally derived from first trimester female human cytotrophoblast cells and immortalized with simian virus 40 large T antigen, this cell line models first-trimester placental extravillous trophoblasts in vitro. HTR-8/SVneo cells will be cultured for 24 hours followed by exposure to cell culture media (control) or 20 µM DCVC for 6 or 12 hours. Cells (cells frozen in cell culture dish) will then be collected (n=5) In the TCE aim of this study, we will utilize one cell model that phenotypically represents an important placental cell population. |
Institute | University of Michigan |
Department | Biomedical Research Core Facilities |
Laboratory | Metabolomics core |
Last Name | Kachman |
First Name | Maureen |
Address | Ann Arbor, MI |
mkachman@med.umich.edu | |
Phone | 734-232-0842 |
Submit Date | 2020-06-08 |
Publications | https://pubmed.ncbi.nlm.nih.gov/31951115/ |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2020-06-18 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002337 | AN002338 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Agilent 1200 | Agilent 1200 |
Column | Phenomenex Luna NH2 (150 x 2.1mm,3um) | Phenomenex Luna NH2 (150 x 2.1mm,3um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Agilent 6520 QTOF | Agilent 6520 QTOF |
Ion Mode | NEGATIVE | NEGATIVE |
Units | area/ug protein | pmol/ug |