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MB Sample ID: SA153398
Local Sample ID: | AA 02030 metabo control 004 |
Subject ID: | SU001747 |
Subject Type: | Fish |
Subject Species: | Danio rerio |
Taxonomy ID: | 7955 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002725 | AN002726 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | Reversed phase |
Chromatography system | Vanquish UHPLC (Thermo Fisher Scientific). | Thermo Vanquish UHPLC |
Column | Waters Xbridge amide HILIC (100 x 2.1mm,3.5um) | Luna C18 LC (150 x 4.6 mm,3um) |
MS Type | ESI | ESI |
MS instrument type | Ion trap | Ion trap |
MS instrument name | Thermo Orbitrap Exploris 480 | Thermo Orbitrap Exploris 480 |
Ion Mode | UNSPECIFIED | UNSPECIFIED |
Units | peak area | peak area |
Chromatography:
Chromatography ID: | CH002011 |
Chromatography Summary: | For this polar metabolite analysis, a hydrophilic interaction chromatography method (HILIC) with an Xbridge amide column (100 x 2.1 mm i.d., 3.5 µm; Waters) was used for compound separation at 25 °C. Mobile phase A: water with 5 mM ammonium acetate (pH 6.8), and mobile phase B: 100 % acetonitrile. Linear gradient was: 0 min, 85% B; 1.5 min, 85% B; 5.5 min, 35% B; 6.9 min, 35% B; 10.5 min, 35% B; 10.6 min, 10% B; 12.5 min, 10% B; 13.5 min, 85% B; 17.9 min, 85% B; 18 min, 85% B; 20 min, 85% B. The flow rate was: 0-5.5 min, 0.11 ml/min; 6.9-10.5 min, 0.13 ml/min; 10.6-17.9 min, 0.25 ml/min; 18-20 min, 0.11 ml/min. |
Instrument Name: | Vanquish UHPLC (Thermo Fisher Scientific). |
Column Name: | Waters Xbridge amide HILIC (100 x 2.1mm,3.5um) |
Column Temperature: | 25 |
Flow Gradient: | 0 min, 85% B; 1.5 min, 85% B; 5.5 min, 35% B; 6.9 min, 35% B; 10.5 min, 35% B; 10.6 min, 10% B; 12.5 min, 10% B; 13.5 min, 85% B; 17.9 min, 85% B; 18 min, 85% B; 20 min, 85% B. |
Flow Rate: | 0-5.5 min, 0.11 ml/min; 6.9-10.5 min, 0.13 ml/min; 10.6-17.9 min, 0.25 ml/min; 18-20 min, 0.11 ml/min |
Solvent A: | 100% water; 5 mM ammonium acetate, pH 6.8 |
Solvent B: | 100% acetonitrile |
Chromatography Type: | HILIC |
Chromatography ID: | CH002012 |
Chromatography Summary: | The analysis of folic acid and its derivatives was performed using Luna column (100 x 3 mm i.d., 3 µm, C18(2), 100 Å, Phenomenex) at 25 °C. Mobile phase A: water with 0.1% formic acid, and mobile phase B: 100 % methanol. Linear gradient was: 0 min, 2% B; 1.5 min, 2% B; 3.0 min, 15% B; 5.5 min, 95% B; 14.5 min, 95% B; 15.0 min, 2% B; 22.0 min, 2% B. The flow rate was 0.150 ml/min. |
Chromatography Comments: | Luna® 3 µm C18(2) 100 Å, LC Column 150 x 4.6 mm, Ea |
Instrument Name: | Thermo Vanquish UHPLC |
Column Name: | Luna C18 LC (150 x 4.6 mm,3um) |
Column Temperature: | 25 |
Flow Gradient: | 0 min, 2% B; 1.5 min, 2% B; 3.0 min, 15% B; 5.5 min, 95% B; 14.5 min, 95% B; 15.0 min, 2% B; 22.0 min, 2% B. |
Flow Rate: | 0.150 ml/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% methanol |
Chromatography Type: | Reversed phase |