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MB Sample ID: SA322373
| Local Sample ID: | 20210625_AJU_M3_lipidomics |
| Subject ID: | SU003073 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004861 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 Infinity UHPLC |
| Column | Thermo Hypersil GOLD C18 (100 x 2.1mm,1.9um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Q Exactive™ Hybrid Quadrupole-Orbitrap MS |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH003669 |
| Methods Filename: | LC_MS_Method_lipid.pdf |
| Instrument Name: | Agilent 1290 Infinity UHPLC |
| Column Name: | Thermo Hypersil GOLD C18 (100 x 2.1mm,1.9um) |
| Column Temperature: | 320℃ |
| Flow Gradient: | 0–5 min, 5% B; 5–15 min, 5–30% B; 15–22 min, 30–90% B; 22–25 min, 90% B; 25–26 min, 90–5% B; 26–30 min, 5% B. Parameters were set as follows: positive mode, spray voltage; 3.8 kV |
| Flow Rate: | 0.2 mL/min |
| Solvent A: | Acetonitrile:Methanol:Water (19:19:2, v/v/v) + 20 mmol/L ammonium formate + 0.1% formic acid (v/v) |
| Solvent B: | 2-propanol + 20 mmol/L ammonium formate + 0.1% formic acid (v/v) |
| Chromatography Type: | Reversed phase |
