Summary of Study ST000054
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000051. The data can be accessed directly via it's Project DOI: 10.21228/M8MS3J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST000054 |
Study Title | Metabolic Microenvironments in Normal Breast and Breast Cancer (MS analysis) |
Study Type | Metabolomic analysis of normal breast tissue |
Study Summary | The purpose of this study was to understand the metabolomic changes in a breast tissue microenvironment at various stages of cancer development and progression (i.e. normal breast, DCIS, and invasive cancer). The goal of this metabolomics pilot and feasibility study was to apply a broad spectrum GC-MS metabolomics method to determine whether changes in normal tissue of women with cancer correlate with progression and or the subtype of the disease. |
Institute | University of North Carolina |
Department | Systems and Translational Sciences |
Laboratory | Sumner Lab |
Last Name | Sumner |
First Name | Susan |
Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
susan_sumner @unc.edu | |
Phone | 704-250-5066 |
Submit Date | 2014-04-30 |
Num Groups | 8 |
Total Subjects | 192 |
Study Comments | sample preparation and analysis randomized with data collection over multiple days |
Publications | 1. Brauer HA, Makowski L, Hoadley KA, Casbas-Hernandez P, Lang LJ, Roman-Perez E, D’Arcy M, Freemerman AJ, Perou CM, Troester MA: Impact of tumor microenvironment and epithelial phenotypes on metabolism in breast cancer. Clin Cancer Res 2012;19:571-585. 2. J Budczies, C Denkert, B Muller, S Brockmoller, F Klauschen, B Gyorffy, M Dietel, C Richter-Ehrenstein, U Marten, R Salek, J Griffin, M Hilvo, M Oresic, G Wohlgemuth, O Fiehn: Remodeling of central metabolism in invasive breast cancer compared to normal breast tissue – a GC-TOFMS based metabolomics study. BMC Genomics 2012; 13:334. |
Raw Data Available | Yes |
Raw Data File Type(s) | cdf |
Uploaded File Size | 8.4 G |
Analysis Type Detail | GC-MS |
Release Date | 2018-08-27 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000092 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Leco Pegasus 4D GC |
Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
MS Type | EI |
MS instrument type | GC x GC-TOF |
MS instrument name | Leco Pegasus 4D GCxGC TOF |
Ion Mode | POSITIVE |
Units | Peak height |
Chromatography:
Chromatography ID: | CH000060 |
Chromatography Summary: | GC/MS analysis is based on Oliver Fiehn methods |
Instrument Name: | Leco Pegasus 4D GC |
Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
Column Pressure: | 10.4 psi |
Column Temperature: | 50C(0.5 min)-330C(@20C/min)-330C(5 min) |
Flow Gradient: | constant flow |
Flow Rate: | 1.0 mL/min |
Injection Temperature: | 250C |
Retention Index: | FAMEs markers (C8-C30) |
Retention Time: | FAMEs markers (C8-C30) |
Analytical Time: | 20 minutes |
Oven Temperature: | 50C(0.5 min)-330C(@20C/min)-330C(5 min) |
Transferline Temperature: | 280C |
Sample Syringe Size: | 10 L |
Chromatography Type: | GC |