Summary of Study ST000091
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000083. The data can be accessed directly via it's Project DOI: 10.21228/M8T884 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000091 |
Study Title | Quantitative Metabolomics by 1H-NMR and LC-MS/MS Confirms Altered Metabolic Pathways in Diabetes |
Study Type | Pre- and Post- insulin study with matched controls |
Study Summary | We obtained plasma samples from 7 c-peptide negative type 1 diabetic individuals (T1D) and 7 non-diabetic controls (Con) that were matched for age (T1D = 31.1±2.9 yrs, Con = 30.2±3.4 yrs), body mass (T1D = 80.2±4.7kg, Con = 81.9±7.4 kg) and BMI (T1D = 26.5±1.2 kg/m2, Con = 25.2±1.3 kg/m2). Type 1 diabetic people were studied while treated with insulin and also after 8 hours of insulin deprivation |
Institute | Mayo Clinic |
Department | Endocrinology |
Last Name | Nair |
First Name | Sreekumaran |
Address | -- |
nair@mayo.edu | |
Phone | -- |
Submit Date | 2014-07-15 |
Num Groups | 2 |
Raw Data Available | No |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2014-07-26 |
Release Version | 1 |
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Combined analysis:
Analysis ID | AN000145 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity |
Column | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Thermo Quantum Ultra |
Ion Mode | POSITIVE |
Units | uM |
Chromatography:
Chromatography ID: | CH000103 |
Chromatography Summary: | High resolution separation was done using an Acquity UPLC system, injecting 1 l of derviatized solution, with a UPLC BEH C18 1.7 micron 2.1150 mm column from Waters. Column flow was set to 400 l/min with a gradient from 99.9%A to 98%B where buffer A is 1% acetonitrile in 0.1% formic acid and buffer B is 100% acetonitrile. A column temp of 43 degrees Celsius and a sample tray temp of 6 degree Celsius. |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
Column Temperature: | 43 |
Flow Gradient: | 99.9% A to 98% B |
Flow Rate: | 401 µl/min |
Solvent A: | 99% water/1% acetonitrile; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |