Summary of Study ST000153
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000129. The data can be accessed directly via it's Project DOI: 10.21228/M85C7W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000153 |
Study Title | Primary T Cell Noxa Knockdown (Donor 8)-II |
Study Summary | Labeling of cells was carried out in triplicate with each sample containing 35e6. Cells were deprived of glucose or glutamine for 1 h then resuspended in 10mM [13C1,2] D-glucose labeled or 4 mM [13C5]-U-glutamine labeled medium, respectively. At this time cells were also activated with 5 ug/ml anti-CD3 and anti-CD28. Cells were labeled (and activated) for 16 h when samples were spun down, and cells were resuspended in 200 ul ice cold methanol. All samples were immediately stored in -80°C. |
Institute | University of Michigan |
Department | Biomedical Research Core Facilities |
Laboratory | Metabolomics core |
Last Name | Kachman |
First Name | Maureen |
Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
mkachman@umich.edu | |
Submit Date | 2015-02-13 |
Num Groups | 3 |
Total Subjects | 16 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Uploaded File Size | 347 M |
Analysis Type Detail | LC-MS |
Release Date | 2015-04-28 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000244 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Agilent 1260 |
Column | Phenomenex Luna NH2 (150 x 1mm,3um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Agilent 6520 QTOF |
Ion Mode | NEGATIVE |
Units | Area |
Chromatography:
Chromatography ID: | CH000169 |
Methods ID: | AQM010 |
Methods Filename: | QTOF-002-HILIC-35min-1mm-No_Insert.m |
Instrument Name: | Agilent 1260 |
Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
Chromatography Type: | HILIC |