Summary of Study ST000153

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000129. The data can be accessed directly via it's Project DOI: 10.21228/M85C7W This work is supported by NIH grant, U2C- DK119886.


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Study IDST000153
Study TitlePrimary T Cell Noxa Knockdown (Donor 8)-II
Study SummaryLabeling of cells was carried out in triplicate with each sample containing 35e6. Cells were deprived of glucose or glutamine for 1 h then resuspended in 10mM [13C1,2] D-glucose labeled or 4 mM [13C5]-U-glutamine labeled medium, respectively. At this time cells were also activated with 5 ug/ml anti-CD3 and anti-CD28. Cells were labeled (and activated) for 16 h when samples were spun down, and cells were resuspended in 200 ul ice cold methanol. All samples were immediately stored in -80°C.
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Submit Date2015-02-13
Num Groups3
Total Subjects16
Raw Data AvailableYes
Raw Data File Type(s)d
Uploaded File Size347 M
Analysis Type DetailLC-MS
Release Date2015-04-28
Release Version1
Maureen Kachman Maureen Kachman application/zip

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Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Glucose label Glutamine label
SA008390S00017304no no
SA008391S00017312no no
SA008392S00017313no yes
SA008393S00017298no yes
SA008394S00017303no yes
SA008395S00017305no yes
SA008396S00017302no yes
SA008397S00017300no yes
SA008398S00017299no yes
SA008399S00017301no yes
SA008400S00017311yes no
SA008401S00017310yes no
SA008402S00017308yes no
SA008403S00017306yes no
SA008404S00017307yes no
SA008405S00017309yes no
Showing results 1 to 16 of 16