Summary of Study ST000252
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000204. The data can be accessed directly via it's Project DOI: 10.21228/M81C7N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000252 |
Study Title | Impact of recurrent hypoglycemia on brain metabolite profile |
Study Type | Insulin treatment in diabetic rats |
Study Summary | The goal of this study is to determine the effect of mild / moderate hypoglycemia on brain metabolism. To achieve this goal insulin-treated diabetic rats will be subjected to recurrent mild / moderate hypoglycemia. |
Institute | University of Florida |
Department | SECIM |
Last Name | Dave |
First Name | Kunjan |
Kdave@med.miami.edu | |
Phone | 305-243-3590 |
Submit Date | 2015-08-06 |
Num Groups | 2 |
Total Subjects | 40 |
Study Comments | Two groups (i) Insulin treated diabetic + Recurrent hypoglycemia, (ii) Insulin treated diabetic + Recurrent hypoglycemia (only insulin injection) + Glucose infusion to maintain glucose to baseline levels.Total 40 samples. Description: Ten hippocampus samples and 10 hypothalamus sample from ITD+RH group of rats.Similarly, Ten hippocampus samples and 10 hypothalamus sample from ITD+RH+Glucose group of rats. |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Uploaded File Size | 4.8 G |
Analysis Type Detail | LC-MS |
Release Date | 2016-12-22 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000398 | AN000399 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak area | Peak Area |
Chromatography:
Chromatography ID: | CH000282 |
Methods Filename: | Metabolomics_LCMSProtocol.pdf |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Flow Rate: | 0.350mL/min-0.600mL/min |
Internal Standard: | Appendix A - Internal Standard Prep GLCMS.pdf |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |