Summary of Study ST000828
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000219. The data can be accessed directly via it's Project DOI: 10.21228/M8359Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000828 |
Study Title | Metabolic analysis of Parp1 ko/wt Saline & Bleo Mouse Lung Fiboblasts and Human IPF & Normal Lung Fiboblasts 3 |
Study Type | MS analysis |
Study Summary | Glycolysis/TCA/Nucleotide analysis (especially interested in alpha-ketoglutarate) and NAD+ and related metabolite analysis for all samples |
Institute | University of Michigan |
Department | Biomedical Research Core Facilities |
Laboratory | Metabolomics core |
Last Name | Kachman |
First Name | Maureen |
Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
mkachman@med.umich.edu | |
Phone | (734) 232-8175 |
Submit Date | 2017-08-02 |
Num Groups | 6 |
Total Subjects | 16 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | GC-MS/LC-MS |
Release Date | 2017-10-11 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN001834 | AN001835 | AN001836 | AN001837 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | Phenyl | Phenyl | HILIC | GC |
Chromatography system | Agilent | Agilent | Agilent | Agilent GC_7890N |
Column | Restek Pinnacle DB Biphenyl (50 x 2.1mm,1.9um,140A) | Restek Pinnacle DB Biphenyl (50 x 2.1mm,1.9um,140A) | Phenomenex Luna NH2 (150 x 1mm,3um) | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
MS Type | ESI | ESI | ESI | EI |
MS instrument type | Triple quadrupole | Triple quadrupole | QTOF | Single quadrupole |
MS instrument name | Agilent 6490A QQQ | Agilent 6490A QQQ | Agilent 6520B QTOF | Agilent 5975 |
Ion Mode | POSITIVE | POSITIVE | NEGATIVE | POSITIVE |
Units | counts | uM | uM | uM |
Chromatography:
Chromatography ID: | CH001312 |
Instrument Name: | Agilent |
Column Name: | Restek Pinnacle DB Biphenyl (50 x 2.1mm,1.9um,140A) |
Column Temperature: | 40 |
Flow Rate: | 200ul/min |
Solvent A: | 100% water; 5 mM ammonium acetate,pH 9.9 |
Solvent B: | 60% acetonitrile/40% isopropanol |
Chromatography Type: | Phenyl |
Chromatography ID: | CH001313 |
Instrument Name: | Agilent |
Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
Flow Rate: | 0.075 mL/min |
Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
Solvent B: | 100% acetonitrile |
Chromatography Type: | HILIC |
Chromatography ID: | CH001314 |
Instrument Name: | Agilent GC_7890N |
Column Name: | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
Chromatography Type: | GC |