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MB Sample ID: SA137931
Local Sample ID: | R6 |
Subject ID: | SU001700 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Female |
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Collection:
Collection ID: | CO001693 |
Collection Summary: | jejunum tissue (30 mg) was transferred to homogenizer (pre-cooled at -20 ℃) and 100 µL of H2O (pre-cooled at 4℃) was added for homogenate for 1 min. 1 μg/mL 4-chloro-phenylalanine and 800 µL methanol solution were added for protein precipitation and vortexed for 10 min. The sample lysates were centrifuged at 30000g for 10 min at 4 °C, and dried under vacuum. It was reconstituted with 100 μL of methanol: water solution (50:50, v/v) before analysis. |
Sample Type: | Intestine |