Summary of Study ST000460
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000354. The data can be accessed directly via it's Project DOI: 10.21228/M8930Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000460 |
Study Title | Effect of ATG7 reconstitution in AGY571 cells |
Study Type | Cells are treated with vehicle or Doxycycline - to induce expression of GFP or ATG7 |
Study Summary | AGY571 cells engineered to express Dox-inducible GFP or ATG7 were treated with 500 ng/mL or 1 ug/mL Doxycycline for 48 hours. Assessing the effects of ATG7 reexpression in B cell lymphoma. |
Institute | University of Florida |
Department | SECIM |
Laboratory | John Cleveland |
Last Name | Fernandez |
First Name | Mario |
Address | 12902 Magnolia Drive, MRC 4-West |
mario.fernandez@moffitt.org | |
Phone | 813-745-5140 |
Submit Date | 2016-09-08 |
Num Groups | 5 |
Total Subjects | 15 |
Study Comments | Groups collected in triplicate per time point, either treated or untreated with Doxycycline. |
Analysis Type Detail | LC-MS |
Release Date | 2020-07-08 |
Release Version | 1 |
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Collection:
Collection ID: | CO000475 |
Collection Summary: | Cells were counted, cells were collected via centrifugation, washed twice with cold 1x PBS, PBS removed and flash frozen. Pellets were saved at -80 C. |
Sample Type: | Cell |
Collection Method: | Pellet |
Collection Location: | Lab |
Collection Frequency: | 48 hours after Dox treatment |
Storage Conditions: | Pellet at -80C |
Storage Vials: | 1.5 mL microfuge tube |
Tissue Cell Identification: | AGY571 cells |