Summary of Study ST000460
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000354. The data can be accessed directly via it's Project DOI: 10.21228/M8930Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000460 |
Study Title | Effect of ATG7 reconstitution in AGY571 cells |
Study Type | Cells are treated with vehicle or Doxycycline - to induce expression of GFP or ATG7 |
Study Summary | AGY571 cells engineered to express Dox-inducible GFP or ATG7 were treated with 500 ng/mL or 1 ug/mL Doxycycline for 48 hours. Assessing the effects of ATG7 reexpression in B cell lymphoma. |
Institute | University of Florida |
Department | SECIM |
Laboratory | John Cleveland |
Last Name | Fernandez |
First Name | Mario |
Address | 12902 Magnolia Drive, MRC 4-West |
mario.fernandez@moffitt.org | |
Phone | 813-745-5140 |
Submit Date | 2016-09-08 |
Num Groups | 5 |
Total Subjects | 15 |
Study Comments | Groups collected in triplicate per time point, either treated or untreated with Doxycycline. |
Analysis Type Detail | LC-MS |
Release Date | 2020-07-08 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000721 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 1290 Infinity |
Column | Waters AccQ Tag (100 x 2mm,1.7um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 6490 QQQ |
Ion Mode | POSITIVE |
Units | nmol/mg protein |
Chromatography:
Chromatography ID: | CH000518 |
Chromatography Summary: | Amino Acid |
Methods Filename: | SB_AA_Assay.pdf |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters AccQ Tag (100 x 2mm,1.7um) |
Column Temperature: | 55 |
Flow Rate: | 0.7 mL/min |
Solvent A: | AccQ·Tag Eluent A |
Solvent B: | accQ·Tag Eluent B |
Chromatography Type: | Reversed phase |