Summary of Study ST001665
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001070. The data can be accessed directly via it's Project DOI: 10.21228/M8D12Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001665 |
Study Title | Branched-chain alpha-ketoacids are preferentially reaminated and activate protein synthesis in the rat heart |
Study Summary | Branched-chain amino acids (BCAA) and their cognate α-ketoacids (BCKA) are elevated in an array of cardiometabolic diseases. Here we demonstrate that the major metabolic fate of uniformly-13C-labeled α-ketoisovalerate ([U-13C]KIV) in the heart is reamination to valine. Activation of cardiac branched-chain α-ketoacid dehydrogenase (BCKDH) by treatment with the BCKDH kinase inhibitor, BT2, does not impede the strong flux of [U-13C]KIV to valine. |
Institute | Duke University |
Last Name | Walejko |
First Name | Jacquelyn |
Address | 300 N Duke St |
jacquelyn.walejko@duke.edu | |
Phone | 9194792304 |
Submit Date | 2021-01-26 |
Analysis Type Detail | GC-MS |
Release Date | 2021-02-17 |
Release Version | 1 |
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Collection:
Collection ID: | CO001735 |
Collection Summary: | Fed male Wistar rats were anesthetized with 5% isoflurane, and isolated hearts were perfused in the Langendorff mode at 37°C with non-recirculating perfusate. The hearts were allowed to beat spontaneously throughout the perfusion. At the end of each perfusion, hearts were immediately freeze-clamped in liquid nitrogen using the Wollenberger technique and stored at -80 oC for further analysis. |
Sample Type: | Heart |