Summary of Study ST001934
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001223. The data can be accessed directly via it's Project DOI: 10.21228/M8MQ47 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001934 |
Study Title | Differential Accumulation of Metabolites and Transcripts Related to Flavonoid, Styrylpyrone, and Galactolipid Biosynthesis in Equisetum Species and Tissue Types |
Study Summary | Members of the genus Equisetum are often referred to as “living fossils”, partly because they are the only extant representatives of the Equisetidae, a subclass that was once prominent in late Paleozoic forests. Several classes of specialized metabolites have been reported to occur in the genus Equisetum. However, while steady progress is being made with identifying individual novel metabolites of Equisetum, few if any analyses have focused on assessing the chemical diversity across the genus. The present study focused on three species: E. hyemale subsp. affine (rough horsetail or scouring rush), which is native to the temperate to artic portions of North America; E. arvense (common horsetail), which is endemic to the arctic and temperate regions of the northern hemisphere; and Equisetum telmateia subsp. braunii (Milde) Hauke (giant horsetail), which is native to western North America. Both below-ground rhizome and above-ground shoot material was harvested from each species, extracted with aqueous methanol, and subjected to non-targeted HPLC-QTOF-MS analysis. This research project was designed to lay the foundation for continued research to capture the metabolic capabilities in the ferns and fern allies. |
Institute | Washington State University |
Department | Institute of Biological Chemistry |
Laboratory | Lange |
Last Name | Lange |
First Name | Mark |
Address | Plant Sciences Building, Pullman, Washington 99164 |
lange-m@wsu.edu | |
Phone | +1-509-335-3794 |
Submit Date | 2021-09-24 |
Num Groups | 6 |
Total Subjects | 30 |
Publications | https://doi.org/10.3390/metabo12050403 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2022-05-09 |
Release Version | 1 |
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Collection:
Collection ID: | CO002005 |
Collection Summary: | E. arvense, E. hyemale and E. telmateia (voucher specimens deposited with the John G. Searle Herbarium of the Field Museum, Chicago, IL, USA) were maintained in a greenhouse under ambient lighting, with supplemental lighting from sodium- vapor lamps. The photosynthetically active radiation varied from 15 to 25 mol m-2 d-1. Temperatures ranged between 22 and 27 °C and the humidity was set to 70 ± 10 %. Five biological replicates (separate plants) were harvested at the same time of day for below-ground rhizomes and above-ground stems of vegetative shoots. Samples were snap-frozen in liquid nitrogen and freeze-dried (aerial parts for 5 days, rhizomes for 7 days). Lyophilized material was submerged in liquid nitrogen, homogenized using mortar and pestle. |
Sample Type: | Tissue homogenate |
Storage Conditions: | -80 °C |