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MB Sample ID: SA153392
Local Sample ID: | DD 02030 metabo reg FA 004 |
Subject ID: | SU001747 |
Subject Type: | Fish |
Subject Species: | Danio rerio |
Taxonomy ID: | 7955 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002725 | AN002726 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | Reversed phase |
Chromatography system | Vanquish UHPLC (Thermo Fisher Scientific). | Thermo Vanquish UHPLC |
Column | Waters Xbridge amide HILIC (100 x 2.1mm,3.5um) | Luna C18 LC (150 x 4.6 mm,3um) |
MS Type | ESI | ESI |
MS instrument type | Ion trap | Ion trap |
MS instrument name | Thermo Orbitrap Exploris 480 | Thermo Orbitrap Exploris 480 |
Ion Mode | UNSPECIFIED | UNSPECIFIED |
Units | peak area | peak area |
MS:
MS ID: | MS002522 |
Analysis ID: | AN002725 |
Instrument Name: | Thermo Orbitrap Exploris 480 |
Instrument Type: | Ion trap |
MS Type: | ESI |
MS Comments: | Xcalibur 4.4 from Thermo Scientific for data acquisition and we use Sieve 2.2 for chromatographic alignment and peak integration.To analyze polar metabolites, the mass spectrometer was equipped with a HESI probe and operated in the positive/negative switching mode. The relevant parameters were as listed: vaporizer temperature, 350 °C; ion transfer tube temperature, 300 °C; sheath gas, 35; auxiliary gas, 7; sweep gas, 1; spray voltage, 3.5 kV for positive mode and 2.5 kV for negative mode; RF-lens (%), 30. The resolution was set at 60,000 (at m/z 200). Automatic maximum injection time (max IT) and automated gain control (AGC) were used. |
Ion Mode: | UNSPECIFIED |
MS ID: | MS002523 |
Analysis ID: | AN002726 |
Instrument Name: | Thermo Orbitrap Exploris 480 |
Instrument Type: | Ion trap |
MS Type: | ESI |
MS Comments: | Xcalibur 4.4 from Thermo Scientific for data acquisition and we use Sieve 2.2 for chromatographic alignment and peak integration.To measure folate derivatives, Orbitrap Exploris 480 was operated in SIM mode (selected ion monitoring) targeting the following ions: folic acid (442.1470), 5-methyltetrahydrofolate (460.1939), and folinic acid (474.1732). |
Ion Mode: | UNSPECIFIED |