Summary of Study ST001665
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001070. The data can be accessed directly via it's Project DOI: 10.21228/M8D12Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001665 |
Study Title | Branched-chain alpha-ketoacids are preferentially reaminated and activate protein synthesis in the rat heart |
Study Summary | Branched-chain amino acids (BCAA) and their cognate α-ketoacids (BCKA) are elevated in an array of cardiometabolic diseases. Here we demonstrate that the major metabolic fate of uniformly-13C-labeled α-ketoisovalerate ([U-13C]KIV) in the heart is reamination to valine. Activation of cardiac branched-chain α-ketoacid dehydrogenase (BCKDH) by treatment with the BCKDH kinase inhibitor, BT2, does not impede the strong flux of [U-13C]KIV to valine. |
Institute | Duke University |
Last Name | Walejko |
First Name | Jacquelyn |
Address | 300 N Duke St |
jacquelyn.walejko@duke.edu | |
Phone | 9194792304 |
Submit Date | 2021-01-26 |
Analysis Type Detail | GC-MS |
Release Date | 2021-02-17 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002717 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 7890B |
Column | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
MS Type | EI |
MS instrument type | Single quadrupole |
MS instrument name | Agilent 5977A |
Ion Mode | UNSPECIFIED |
Units | Concentration of 13C (uM) |
MS:
MS ID: | MS002514 |
Analysis ID: | AN002717 |
Instrument Name: | Agilent 5977A |
Instrument Type: | Single quadrupole |
MS Type: | EI |
MS Comments: | Masshunter used for data acquisition and processing. |
Ion Mode: | UNSPECIFIED |