Summary of Study ST001105
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000739. The data can be accessed directly via it's Project DOI: 10.21228/M85675 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001105 |
Study Title | Retinal ganglion cells lipid profiling |
Study Summary | The aim of this project was to characterize lipid profiles of the retinal ganglion cells (RGCs) with different regenerative capacity. RGCs were FACS-sorted (7,000 cells/sample) from mice of OPN4 Cre tdT and Thy1-CFP genotype. The treatment conditions included intact, optic nerve crush (ONC) and ONC plus CNTF to promote regeneration. Samples were then subject to lipid extraction and analyzed using LC-MS/MS, followed by identification and relative quantification in LipidSearch software. |
Institute | University of Miami |
Department | Ophthalmology, Bascom Palmer Eye Institute |
Laboratory | Sanjoy K. Bhattacharya |
Last Name | Bhattacharya |
First Name | Sanjoy |
Address | 1638 NW 10th Ave, Miami, FL 33136 |
sbhattacharya@med.miami.edu | |
Phone | +1 (305) 4824103 |
Submit Date | 2018-11-26 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2019-01-22 |
Release Version | 1 |
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Project:
Project ID: | PR000739 |
Project DOI: | doi: 10.21228/M85675 |
Project Title: | Retinal ganglion cells lipid profiling |
Project Summary: | The aim of this project was to characterize lipid profiles of the retinal ganglion cells (RGCs) with different regenerative capacity. RGCs were FACS-sorted (7,000 cells/sample) from mice of OPN4 Cre tdT and Thy1-CFP genotype. The treatment conditions included intact, optic nerve crush (ONC) and ONC plus CNTF to promote regeneration. Samples were then subject to lipid extraction and analyzed using LC-MS/MS, followed by identification and relative quantification in LipidSearch software. |
Institute: | University of Miami |
Department: | Ophthalmology, Bascom Palmer Eye Institute |
Laboratory: | Sanjoy K. Bhattacharya |
Last Name: | Bhattacharya |
First Name: | Sanjoy |
Address: | 900 NW 17th St, Miami, FL 33136 |
Email: | sbhattacharya@med.miami.edu |
Phone: | +1 (305) 4824103 |
Funding Source: | NEI/NIH U01EY027257 |
Contributors: | Kevin (Kyung) Park, Sanjoy K. Bhattacharya, Ana Carolina Ayupe Oliveira Beckedorff, Anna Trzeciecka |