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MB Sample ID: SA113119
| Local Sample ID: | POS_45 |
| Subject ID: | SU001461 |
| Subject Type: | Mammal |
| Subject Species: | Myotis lucifugus |
| Taxonomy ID: | 59463 |
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Sample Preparation:
| Sampleprep ID: | SP001469 |
| Sampleprep Summary: | Liver samples (~10 mg) were homogenized with cold methanol (300 μl) containing internal standards (10 μl SPLASH® Lipidomix®), 10 μl was removed for protein quantification, and the tissue homogenate was incubated on ice for 5 min. Chilled chloroform (600 μl) was added to samples and followed by shaking (vortex 30 sec) and further incubation on ice (10 min). Chilled water (300 μl) was added to samples followed by shaking (vortex 30 sec) and incubation on ice (10 min), then centrifuged for 10 min (10,000 x g, 4°C) to separate layers. The lower organic phase was separated, evaporated under vacuum, and reconstituted in 200 μl isopropanol (IPA):acetonitrile (ACN):H2O (2:1:1) for analysis. |
| Processing Storage Conditions: | On ice |
| Extract Storage: | On ice |
