Summary of Study ST000497
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000374. The data can be accessed directly via it's Project DOI: 10.21228/M8Q60G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000497 |
Study Title | M-CMV infected Nicotiana tabacum |
Study Type | LC-MS & GC-MS |
Study Summary | M-CMV-infected N. tabacum plants with six symptoms (vein clearing, mosaic, chlorosis, partial green recovery, complete green recovery and secondary mosaic) were analyzed by LC-MS & GC-MS. In addition, the pathogenesis biomarker might be found by this untargeted global metabolomic analysis. |
Institute | Chinese Academy of Inspection and Quarantine |
Department | Institute of Plant Quarantine of China |
Last Name | Lei |
First Name | Rong |
Address | Rong Hua Nan Lu 11, Yi Zhuang, Beijing, China |
leirong2009@gmail.com | |
Phone | 86-10-53897620 |
Submit Date | 2016-10-23 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | GC-MS/LC-MS |
Release Date | 2017-11-20 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000525 |
Sampleprep Summary: | Samples were prepared using the automated MicroLab STAR® system (Hamilton Company, Salt Lake City, UT). A 6-mg quantity of each lyophilized leaf sample was thawed on ice and extracted using the automated MicroLab STAR system in 400 L of methanol (LC-MS Ultra CHROMASOLV®, Riedel-de Haёn) containing recovery standards. The samples were prepared using a proprietary series of organic and aqueous extractions to remove the protein fraction while maximizing the recovery of small molecules. The resulting extract was divided into two fractions; one for analysis by liquid chromatography (LC) and one for analysis by gas chromatography (GC). The samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. Each sample was then frozen and dried under vacuum, followed by preparation for either LC/MS or GC/MS. |