Summary of Study ST002744
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001509. The data can be accessed directly via it's Project DOI: 10.21228/M8N71K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002744 |
Study Title | Biomolecular condensates create phospholipid-enriched microenvironments (Part 4) |
Study Summary | Proteins and RNA are able to phase separate from the aqueous cellular environment to form sub-cellular compartments called condensates. This process results in a protein-RNA mixture that is chemically distinct from the surrounding aqueous phase. Here we use mass spectrometry to characterize the metabolomes of condensates. To test this, we prepared mixtures of phase-separated proteins and cellular metabolites and identified metabolites enriched in the condensate phase. Here, we confirmed the presence of lipids in MED1 samples and condensates using reversed-phase LC-MS. |
Institute | Cornell University |
Last Name | Dumelie |
First Name | Jason |
Address | 1300 York Ave, LC-524, New York City, NY |
srj2003@med.cornell.edu | |
Phone | 6466590174 |
Submit Date | 2023-06-05 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzdata.xml |
Analysis Type Detail | LC-MS |
Release Date | 2023-07-07 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP002857 |
Sampleprep Summary: | Dried-down extracts were reconstituted in 150 µl 70% acetonitrile, at a relative protein concentration of ~ 2 µg/µl. These were stored at -20C until used. In these LC-MS experiments, 4 µl of reconstituted extract was injected for LC/MS-based targeted metabolite profiling for each sample. |
Processing Storage Conditions: | Described in summary |
Extract Storage: | -20℃ |