Summary of Study ST002972
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001849. The data can be accessed directly via it's Project DOI: 10.21228/M8QB0V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002972 |
Study Title | Untargeted metabolomics of alkane producing strains at time 5 h of bioconversion |
Study Summary | We demonstrated applications of this system using three different engineered Escherichia coli requiring different cofactors (fatty alcohol, bioluminescence light, and alkane biosynthesis). The results showed that the XR/lactose system could enhance the production of each system by ~2-4-fold. Untargeted metabolomics and transcriptomics analyses revealed that XR only enhanced pathways related to specific cofactor utilization. |
Institute | Vidyasirimedhi Institute of Science and Technology |
Last Name | Sutthaphirom |
First Name | Chalermroj |
Address | 555, Moo 1, Payubnai, Wangchan, Rayong, 21210, Thailand |
chalermroj.s_s19@vistec.ac.th | |
Phone | (+66)063-9639915 |
Submit Date | 2023-11-06 |
Num Groups | 2 |
Total Subjects | 8 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2023-11-20 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP003091 |
Sampleprep Summary: | The pellets were resuspended in 2 mL of cold 100% methanol (-80˚C) (HPLC grade). The samples were frozen in liquid nitrogen and thawed on ice for 3 cycles for extracting intracellular metabolites. The suspensions were centrifuged at 18,800´g at -9˚C for 30 min. The supernatant was transferred into a new 15 mL falcon tube, frozen in liquid nitrogen, and freeze-dried at -80˚C, 1 mbar, for 16 hours. The dried samples were maintained on ice, resuspended in 500 µL of cold 50% ACN/water (HPLC grade), and centrifuged for 30 min at 18,800´g and at -9˚C. The supernatant was collected and stored at -80˚C until analyzing with mass spectrometry. |
Processing Storage Conditions: | On ice |
Extraction Method: | Cold methanol |
Extract Storage: | -80℃ |
Sample Resuspension: | 500 µL of 50% ACN/water |