Summary of Study ST002972
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001849. The data can be accessed directly via it's Project DOI: 10.21228/M8QB0V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002972 |
| Study Title | Untargeted metabolomics of alkane producing strains at time 5 h of bioconversion |
| Study Summary | We demonstrated applications of this system using three different engineered Escherichia coli requiring different cofactors (fatty alcohol, bioluminescence light, and alkane biosynthesis). The results showed that the XR/lactose system could enhance the production of each system by ~2-4-fold. Untargeted metabolomics and transcriptomics analyses revealed that XR only enhanced pathways related to specific cofactor utilization. |
| Institute | Vidyasirimedhi Institute of Science and Technology |
| Last Name | Sutthaphirom |
| First Name | Chalermroj |
| Address | 555, Moo 1, Payubnai, Wangchan, Rayong, 21210, Thailand |
| chalermroj.s_s19@vistec.ac.th | |
| Phone | (+66)063-9639915 |
| Submit Date | 2023-11-06 |
| Num Groups | 2 |
| Total Subjects | 8 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-11-20 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR003094 |
| Treatment Summary: | Cells were grown in TB media and induced the protein expression with lactose for 16 h at 25˚C. They were harvested by centrifugation and resuspended in Kpi buffer. Cells of OD60/mL were incubated with 10 mM lactose and 5 mM myristic acid at 25˚C for 5 h in the presence of blue light before collection. |
| Treatment Compound: | 10 mM lactose, 5 mM myristic acid |
| Cell Growth Container: | 20 mL glass vial |
| Cell Envir Cond: | 25˚C with blue light |
