Summary of Study ST001221
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000819. The data can be accessed directly via it's Project DOI: 10.21228/M8TD5C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001221 |
Study Title | Effects of selenate exposure on the bumble bee metabolome |
Study Summary | We exposed worker bumble bees to sodium selenate or control through diet. We then used LCMS untargeted metabolomics on pools of 3 bees each. |
Institute | University of California, Riverside |
Last Name | Rothman |
First Name | Jason |
Address | 900 University Ave., Riverside, CA, 91766, USA |
jroth002@ucr.edu | |
Phone | 9518275817 |
Submit Date | 2019-07-17 |
Raw Data File Type(s) | raw(Waters) |
Analysis Type Detail | GC-MS |
Release Date | 2020-03-03 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001296 |
Sampleprep Summary: | We then pooled three bee abdomens from each cage, freeze-dried the samples, and homogenized the abdomens to a fine powder at 4° C using a bead mill homogenizer. Next, we extracted 10-12 mg of the powder in a 1.5 mL tube with 100 µL of ice-cold extraction solvent (30:30:20:20 acetonitrile:methanol:water:isopropanol) per 1 mg of tissue. We sonicated the samples for 5 minutes in an ice bath, then vortexed them for 30 min at 4° C. Lastly, we centrifuged the samples at 16,000 x g for 15 min at 4° C. |