Summary of Study ST001221

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000819. The data can be accessed directly via it's Project DOI: 10.21228/M8TD5C This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST001221
Study Title	Effects of selenate exposure on the bumble bee metabolome
Study Summary	We exposed worker bumble bees to sodium selenate or control through diet. We then used LCMS untargeted metabolomics on pools of 3 bees each.
Institute	University of California, Riverside
Last Name	Rothman
First Name	Jason
Address	900 University Ave., Riverside, CA, 91766, USA
Email	jroth002@ucr.edu
Phone	9518275817
Submit Date	2019-07-17
Raw Data File Type(s)	raw(Waters)
Analysis Type Detail	GC-MS
Release Date	2020-03-03
Release Version	1

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Treatment:

Treatment ID:	TR001303
Treatment Summary:	We obtained five commercial Bombus impatiens colonies that each contained approximately 50 workers, a gravid queen, pollen and a Biogluc® feeder from the Biobest Group (Biobest USA Inc., Romulus, MI). We immediately replaced the Biogluc with sterile 60% sucrose and allowed the bees access ad libitum. We also provided the colony with pollen patties ad libitum and kept the colonies at 29 °C under constant darkness at the University of California, Riverside. We allowed the colonies to grow undisturbed for two weeks before starting the experiment. Once the colonies had populated, we transferred 10 bees (two cohorts of five) to 475 mL polypropylene containers (WebstaurantStore, Lancaster, PA) and reared them at 29 °C under 24-hour darkness. We then exposed the bees to treatments consisting of 60% sucrose spiked with 0.6 mg/kg sodium selenate and used unspiked 60% sucrose as a control.

Treatment ID:

TR001303

Treatment Summary:

We obtained five commercial Bombus impatiens colonies that each contained approximately 50 workers, a gravid queen, pollen and a Biogluc® feeder from the Biobest Group (Biobest USA Inc., Romulus, MI). We immediately replaced the Biogluc with sterile 60% sucrose and allowed the bees access ad libitum. We also provided the colony with pollen patties ad libitum and kept the colonies at 29 °C under constant darkness at the University of California, Riverside. We allowed the colonies to grow undisturbed for two weeks before starting the experiment. Once the colonies had populated, we transferred 10 bees (two cohorts of five) to 475 mL polypropylene containers (WebstaurantStore, Lancaster, PA) and reared them at 29 °C under 24-hour darkness. We then exposed the bees to treatments consisting of 60% sucrose spiked with 0.6 mg/kg sodium selenate and used unspiked 60% sucrose as a control.