Summary of Study ST001881
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001186. The data can be accessed directly via it's Project DOI: 10.21228/M8DD61 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001881 |
| Study Title | NMR Predator Cues Target Signaling Pathways in Toxic Algal Metabolome (Non-polar metabolites) |
| Study Type | 1H NMR Metabolomics to elucidate signaling pathway |
| Study Summary | Metabolomics investigation of the phytoplankton Alexandrium minutum with and without copepod cues in order to explore cell signaling involved in toxin induction. |
| Institute | Georgia Institute of Technology |
| Department | School of Biological Sciences, School of Chemistry and Biochemistry, Center for Microbial Dynamics and Infection, Parker H. Petit Institute for Bioengineering and Bioscience |
| Laboratory | Kubanek Lab |
| Last Name | Brown |
| First Name | Emily |
| Address | 950 Atlantic Dr Atlanta, Georgia USA 30332 |
| julia.kubanek@biosci.gatech.edu | |
| Phone | 404-894-8424 |
| Submit Date | 2021-07-19 |
| Num Groups | 2 |
| Total Subjects | 40 |
| Study Comments | Part 2 of 3. This part includes NMR analysis of non-polar metabolites using oPLSDA. Parts 1 and 3 inlcude NMR analysis of polar metabolites and the corresponding mass spectrometry metabolomics for both polar and non-polar metabolites. |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2021-07-27 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP001964 |
| Sampleprep Summary: | Non-polar extracts were dissolved at a concentration equivalent to 2.27 x 10^7 A. minutum cells mL-1 in dimethyl sulfoxide d6 (99.9% atom d6-DMSO; Cambridge Isotope Labs) containing 0.1% trimethylsilane (TMS), as internal standard. The media blank extract was prepared in the smallest volume of solvent possible (175 µL). The non-polar extracts were transferred to 3 mm NMR tubes, and analyzed using a Bruker Avance IIIHD 800 MHz NMR spectrometer equipped with a 3 mm triple resonance broadband cryoprobe. Spectra of non-polar extracts were acquired using a simple 1H NMR experiment (Bruker zg30 pulse sequence) with a spectral width of 9.6 kHz centered at 4400.00 Hz over 320 scans. One treatment replicate was lost and therefore its spectrum was not acquired. |
| Sampleprep Protocol Filename: | Predator Cues Target Signaling Pathways in Toxic Algal Metabolome_protocol.pdf |
| Extract Storage: | -80℃ |
